Kitasatospora atroaurantiaca
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6749 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A561EJ01|A0A561EJ01_9ACTN Uncharacterized protein OS=Kitasatospora atroaurantiaca OX=285545 GN=FB465_0486 PE=4 SV=1
MM1 pKa = 7.61 LVTDD5 pKa = 5.11 IDD7 pKa = 4.04 DD8 pKa = 4.09 LSYY11 pKa = 10.97 VAVMYY16 pKa = 10.0 PGLDD20 pKa = 3.13 VYY22 pKa = 11.15 LADD25 pKa = 4.95 LADD28 pKa = 4.1 VLDD31 pKa = 4.9 LDD33 pKa = 4.97 FDD35 pKa = 4.11 THH37 pKa = 6.16 TSLIDD42 pKa = 3.41 HH43 pKa = 7.28 PEE45 pKa = 3.66 YY46 pKa = 9.42 GARR49 pKa = 11.84 MRR51 pKa = 11.84 EE52 pKa = 4.04 HH53 pKa = 7.34 AEE55 pKa = 3.96 NTGLGPDD62 pKa = 4.07 GIEE65 pKa = 4.18 FQDD68 pKa = 4.55 LSWPGG73 pKa = 3.07
Molecular weight: 8.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.681
IPC_protein 3.656
Toseland 3.439
ProMoST 3.859
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.656
Rodwell 3.49
Grimsley 3.35
Solomon 3.643
Lehninger 3.605
Nozaki 3.808
DTASelect 4.062
Thurlkill 3.516
EMBOSS 3.656
Sillero 3.783
Patrickios 0.769
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.731
Protein with the highest isoelectric point:
>tr|A0A561ESN3|A0A561ESN3_9ACTN Uncharacterized protein OS=Kitasatospora atroaurantiaca OX=285545 GN=FB465_3701 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.43 GRR40 pKa = 11.84 SSISAA45 pKa = 3.4
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.647
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.369
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.218
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6749
0
6749
2226480
29
4030
329.9
35.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.802 ± 0.051
0.803 ± 0.008
5.617 ± 0.026
5.462 ± 0.032
2.716 ± 0.016
9.373 ± 0.03
2.235 ± 0.015
3.262 ± 0.021
2.089 ± 0.026
10.654 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.642 ± 0.011
1.927 ± 0.023
6.185 ± 0.028
3.098 ± 0.018
7.638 ± 0.04
5.294 ± 0.024
6.203 ± 0.035
8.288 ± 0.031
1.564 ± 0.013
2.148 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here