Capybara microvirus Cap1_SP_143
Average proteome isoelectric point is 5.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVP9|A0A4V1FVP9_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_143 OX=2585389 PE=4 SV=1
MM1 pKa = 7.48 LRR3 pKa = 11.84 TNLTLSKK10 pKa = 10.45 RR11 pKa = 11.84 IFSDD15 pKa = 3.05 SGSRR19 pKa = 11.84 FVDD22 pKa = 4.01 TYY24 pKa = 11.26 RR25 pKa = 11.84 PVINADD31 pKa = 2.61 GHH33 pKa = 5.97 MDD35 pKa = 5.32 LIQSGKK41 pKa = 9.73 KK42 pKa = 9.79 DD43 pKa = 3.58 VYY45 pKa = 11.61 ADD47 pKa = 3.5 IQSHH51 pKa = 6.66 AEE53 pKa = 3.8 EE54 pKa = 5.12 CDD56 pKa = 3.18 INLLIEE62 pKa = 4.86 KK63 pKa = 9.85 YY64 pKa = 10.94 QMTGDD69 pKa = 3.49 PAVINKK75 pKa = 9.78 RR76 pKa = 11.84 EE77 pKa = 3.97 GLYY80 pKa = 10.69 LDD82 pKa = 3.73 VTDD85 pKa = 4.15 MPKK88 pKa = 9.82 TYY90 pKa = 10.94 AEE92 pKa = 3.8 IYY94 pKa = 10.02 EE95 pKa = 4.37 RR96 pKa = 11.84 VAQAQDD102 pKa = 3.5 VFSKK106 pKa = 11.01 LPLSVKK112 pKa = 10.14 EE113 pKa = 4.86 KK114 pKa = 10.88 FDD116 pKa = 4.02 FDD118 pKa = 3.91 PAKK121 pKa = 10.52 FFASLGSKK129 pKa = 7.46 EE130 pKa = 4.22 TSALLAEE137 pKa = 4.76 TFNASPASDD146 pKa = 4.08 AGDD149 pKa = 3.67 ASSSLSSNEE158 pKa = 3.51 AATPTLSVEE167 pKa = 4.22 GDD169 pKa = 3.15
Molecular weight: 18.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.615
IPC2_protein 4.558
IPC_protein 4.495
Toseland 4.317
ProMoST 4.546
Dawson 4.457
Bjellqvist 4.635
Wikipedia 4.355
Rodwell 4.329
Grimsley 4.228
Solomon 4.457
Lehninger 4.406
Nozaki 4.571
DTASelect 4.774
Thurlkill 4.329
EMBOSS 4.368
Sillero 4.609
Patrickios 4.012
IPC_peptide 4.457
IPC2_peptide 4.596
IPC2.peptide.svr19 4.565
Protein with the highest isoelectric point:
>tr|A0A4P8W7Z4|A0A4P8W7Z4_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_143 OX=2585389 PE=3 SV=1
MM1 pKa = 7.52 ACSNPNRR8 pKa = 11.84 IFVIGKK14 pKa = 8.72 NPKK17 pKa = 8.28 TGKK20 pKa = 10.14 KK21 pKa = 10.11 KK22 pKa = 10.3 IVFCSRR28 pKa = 11.84 FVRR31 pKa = 11.84 AVGVKK36 pKa = 10.36 DD37 pKa = 3.94 DD38 pKa = 4.04 GSVDD42 pKa = 3.26 RR43 pKa = 11.84 FYY45 pKa = 11.46 EE46 pKa = 4.34 DD47 pKa = 3.56 PEE49 pKa = 4.17 KK50 pKa = 11.34 DD51 pKa = 3.37 KK52 pKa = 11.76 VPFTYY57 pKa = 10.09 RR58 pKa = 11.84 SRR60 pKa = 11.84 LYY62 pKa = 10.56 KK63 pKa = 10.35 FVVSDD68 pKa = 4.62 YY69 pKa = 11.25 IDD71 pKa = 3.87 VPCGKK76 pKa = 10.27 CLGCRR81 pKa = 11.84 LAYY84 pKa = 10.14 SKK86 pKa = 10.63 QWTSRR91 pKa = 11.84 IMLEE95 pKa = 4.74 LPYY98 pKa = 9.92 HH99 pKa = 5.37 DD100 pKa = 5.01 HH101 pKa = 6.18 SWFVTLTYY109 pKa = 10.78 DD110 pKa = 5.53 DD111 pKa = 3.66 EE112 pKa = 4.6 HH113 pKa = 8.71 LPINEE118 pKa = 3.95 FTDD121 pKa = 3.93 PVTSEE126 pKa = 4.12 VGEE129 pKa = 4.24 STTLVKK135 pKa = 10.52 RR136 pKa = 11.84 HH137 pKa = 5.37 VQLFMKK143 pKa = 10.1 KK144 pKa = 9.78 LRR146 pKa = 11.84 NAYY149 pKa = 8.88 PDD151 pKa = 3.15 NHH153 pKa = 7.27 LMYY156 pKa = 10.2 FFAGEE161 pKa = 3.89 YY162 pKa = 9.88 GDD164 pKa = 3.57 RR165 pKa = 11.84 SRR167 pKa = 11.84 RR168 pKa = 11.84 PHH170 pKa = 4.59 YY171 pKa = 10.08 HH172 pKa = 7.17 AIIFDD177 pKa = 4.31 LPLDD181 pKa = 4.04 DD182 pKa = 4.97 LVFYY186 pKa = 8.76 KK187 pKa = 10.47 TSPNGDD193 pKa = 2.98 IYY195 pKa = 11.22 FNSPKK200 pKa = 10.35 LEE202 pKa = 4.65 KK203 pKa = 9.62 IWSHH207 pKa = 5.58 GFVVVANVTYY217 pKa = 10.99 ASAAYY222 pKa = 5.99 TARR225 pKa = 11.84 YY226 pKa = 8.85 IMKK229 pKa = 9.18 KK230 pKa = 10.34 QNVSDD235 pKa = 3.96 EE236 pKa = 3.91 NSIYY240 pKa = 10.52 ARR242 pKa = 11.84 KK243 pKa = 10.05 NIEE246 pKa = 3.92 PEE248 pKa = 3.99 FCLMSQKK255 pKa = 10.41 PSIGRR260 pKa = 11.84 RR261 pKa = 11.84 YY262 pKa = 10.21 FEE264 pKa = 4.82 DD265 pKa = 3.92 HH266 pKa = 7.27 PDD268 pKa = 3.77 VYY270 pKa = 11.44 DD271 pKa = 4.65 LDD273 pKa = 4.24 YY274 pKa = 11.5 LSLAGGYY281 pKa = 8.48 RR282 pKa = 11.84 ASIPKK287 pKa = 10.04 YY288 pKa = 10.14 FDD290 pKa = 3.42 YY291 pKa = 11.28 LLEE294 pKa = 4.44 KK295 pKa = 10.55 VDD297 pKa = 4.35 AEE299 pKa = 3.82 RR300 pKa = 11.84 LQAIKK305 pKa = 10.36 EE306 pKa = 4.01 EE307 pKa = 4.24 RR308 pKa = 11.84 KK309 pKa = 9.94 FFAQHH314 pKa = 6.4 LANIKK319 pKa = 10.36 EE320 pKa = 4.3 SMHH323 pKa = 5.7 TYY325 pKa = 10.4 DD326 pKa = 4.71 RR327 pKa = 11.84 EE328 pKa = 4.23 SHH330 pKa = 6.52 FDD332 pKa = 3.69 VIHH335 pKa = 6.61 EE336 pKa = 4.21 NLVSRR341 pKa = 11.84 SKK343 pKa = 10.94 HH344 pKa = 4.79 LAKK347 pKa = 10.69 DD348 pKa = 3.56 LL349 pKa = 4.02
Molecular weight: 40.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.148
IPC2_protein 7.483
IPC_protein 7.424
Toseland 7.278
ProMoST 7.922
Dawson 8.097
Bjellqvist 8.317
Wikipedia 8.053
Rodwell 8.112
Grimsley 7.366
Solomon 8.185
Lehninger 8.2
Nozaki 8.419
DTASelect 8.17
Thurlkill 8.214
EMBOSS 8.317
Sillero 8.492
Patrickios 4.088
IPC_peptide 8.185
IPC2_peptide 7.366
IPC2.peptide.svr19 7.52
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1478
87
551
295.6
33.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.593 ± 2.225
0.609 ± 0.338
6.428 ± 0.975
4.736 ± 0.578
4.736 ± 0.98
5.683 ± 0.776
2.165 ± 0.67
5.074 ± 0.704
4.804 ± 1.016
7.172 ± 0.617
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.639 ± 0.724
6.157 ± 1.564
4.668 ± 0.612
3.518 ± 0.552
4.398 ± 0.696
10.352 ± 1.6
5.548 ± 0.594
6.089 ± 0.579
1.218 ± 0.397
5.413 ± 0.582
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here