Amino acid dipepetide frequency for Sphaerotilus phage vB_SnaP-R1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.857AlaAla: 17.857 ± 2.26
1.221AlaCys: 1.221 ± 0.539
6.716AlaAsp: 6.716 ± 0.695
6.563AlaGlu: 6.563 ± 0.977
3.892AlaPhe: 3.892 ± 0.514
9.692AlaGly: 9.692 ± 1.362
2.518AlaHis: 2.518 ± 0.452
3.968AlaIle: 3.968 ± 0.508
6.181AlaLys: 6.181 ± 0.856
11.828AlaLeu: 11.828 ± 0.808
2.747AlaMet: 2.747 ± 0.434
4.197AlaAsn: 4.197 ± 0.833
4.197AlaPro: 4.197 ± 0.795
5.8AlaGln: 5.8 ± 0.774
4.808AlaArg: 4.808 ± 0.665
6.334AlaSer: 6.334 ± 0.886
7.097AlaThr: 7.097 ± 1.436
8.394AlaVal: 8.394 ± 0.807
1.908AlaTrp: 1.908 ± 0.427
3.281AlaTyr: 3.281 ± 0.474
0.0AlaXaa: 0.0 ± 0.0
Cys
0.687CysAla: 0.687 ± 0.417
0.382CysCys: 0.382 ± 0.275
0.305CysAsp: 0.305 ± 0.15
0.916CysGlu: 0.916 ± 0.316
0.458CysPhe: 0.458 ± 0.211
1.297CysGly: 1.297 ± 0.536
0.076CysHis: 0.076 ± 0.095
0.687CysIle: 0.687 ± 0.256
0.229CysLys: 0.229 ± 0.119
0.687CysLeu: 0.687 ± 0.238
0.382CysMet: 0.382 ± 0.192
0.229CysAsn: 0.229 ± 0.131
0.992CysPro: 0.992 ± 0.43
0.229CysGln: 0.229 ± 0.122
0.839CysArg: 0.839 ± 0.266
0.458CysSer: 0.458 ± 0.205
1.221CysThr: 1.221 ± 0.327
0.458CysVal: 0.458 ± 0.141
0.382CysTrp: 0.382 ± 0.159
0.382CysTyr: 0.382 ± 0.15
0.0CysXaa: 0.0 ± 0.0
Asp
6.944AspAla: 6.944 ± 0.665
1.145AspCys: 1.145 ± 0.333
2.747AspAsp: 2.747 ± 0.433
2.747AspGlu: 2.747 ± 0.461
1.679AspPhe: 1.679 ± 0.3
4.731AspGly: 4.731 ± 0.639
0.916AspHis: 0.916 ± 0.266
2.976AspIle: 2.976 ± 0.517
3.129AspLys: 3.129 ± 0.502
5.647AspLeu: 5.647 ± 0.516
0.992AspMet: 0.992 ± 0.244
1.755AspAsn: 1.755 ± 0.365
3.205AspPro: 3.205 ± 0.631
1.908AspGln: 1.908 ± 0.386
3.739AspArg: 3.739 ± 0.574
2.824AspSer: 2.824 ± 0.43
4.197AspThr: 4.197 ± 0.645
3.205AspVal: 3.205 ± 0.497
0.763AspTrp: 0.763 ± 0.245
1.526AspTyr: 1.526 ± 0.351
0.0AspXaa: 0.0 ± 0.0
Glu
7.86GluAla: 7.86 ± 0.828
0.611GluCys: 0.611 ± 0.203
2.9GluAsp: 2.9 ± 0.492
2.518GluGlu: 2.518 ± 0.492
3.358GluPhe: 3.358 ± 0.601
3.892GluGly: 3.892 ± 0.63
1.526GluHis: 1.526 ± 0.364
2.9GluIle: 2.9 ± 0.475
2.442GluLys: 2.442 ± 0.462
6.105GluLeu: 6.105 ± 0.679
1.45GluMet: 1.45 ± 0.309
1.832GluAsn: 1.832 ± 0.486
1.374GluPro: 1.374 ± 0.322
3.129GluGln: 3.129 ± 0.592
3.663GluArg: 3.663 ± 0.594
2.442GluSer: 2.442 ± 0.427
2.442GluThr: 2.442 ± 0.39
4.96GluVal: 4.96 ± 0.688
0.839GluTrp: 0.839 ± 0.273
2.137GluTyr: 2.137 ± 0.434
0.0GluXaa: 0.0 ± 0.0
Phe
3.663PheAla: 3.663 ± 0.438
0.153PheCys: 0.153 ± 0.105
2.671PheAsp: 2.671 ± 0.489
2.137PheGlu: 2.137 ± 0.494
0.992PhePhe: 0.992 ± 0.373
1.984PheGly: 1.984 ± 0.513
0.305PheHis: 0.305 ± 0.154
1.45PheIle: 1.45 ± 0.364
1.603PheLys: 1.603 ± 0.452
2.137PheLeu: 2.137 ± 0.363
0.763PheMet: 0.763 ± 0.223
1.526PheAsn: 1.526 ± 0.333
1.221PhePro: 1.221 ± 0.27
1.908PheGln: 1.908 ± 0.356
2.06PheArg: 2.06 ± 0.418
2.442PheSer: 2.442 ± 0.427
1.603PheThr: 1.603 ± 0.368
1.603PheVal: 1.603 ± 0.332
0.382PheTrp: 0.382 ± 0.19
0.763PheTyr: 0.763 ± 0.213
0.0PheXaa: 0.0 ± 0.0
Gly
8.471GlyAla: 8.471 ± 1.318
0.763GlyCys: 0.763 ± 0.269
4.502GlyAsp: 4.502 ± 0.666
4.579GlyGlu: 4.579 ± 0.543
2.976GlyPhe: 2.976 ± 0.457
7.937GlyGly: 7.937 ± 2.37
0.687GlyHis: 0.687 ± 0.231
4.426GlyIle: 4.426 ± 0.435
3.968GlyLys: 3.968 ± 0.601
6.334GlyLeu: 6.334 ± 0.658
1.908GlyMet: 1.908 ± 0.338
3.129GlyAsn: 3.129 ± 0.408
4.426GlyPro: 4.426 ± 0.973
2.976GlyGln: 2.976 ± 0.436
5.189GlyArg: 5.189 ± 0.618
5.266GlySer: 5.266 ± 1.127
6.716GlyThr: 6.716 ± 2.01
5.723GlyVal: 5.723 ± 0.664
1.145GlyTrp: 1.145 ± 0.298
1.755GlyTyr: 1.755 ± 0.3
0.0GlyXaa: 0.0 ± 0.0
His
1.679HisAla: 1.679 ± 0.397
0.153HisCys: 0.153 ± 0.094
1.603HisAsp: 1.603 ± 0.422
1.526HisGlu: 1.526 ± 0.335
0.458HisPhe: 0.458 ± 0.198
1.603HisGly: 1.603 ± 0.325
0.382HisHis: 0.382 ± 0.168
0.992HisIle: 0.992 ± 0.324
1.374HisLys: 1.374 ± 0.365
1.679HisLeu: 1.679 ± 0.34
0.534HisMet: 0.534 ± 0.196
0.839HisAsn: 0.839 ± 0.276
1.526HisPro: 1.526 ± 0.333
0.534HisGln: 0.534 ± 0.185
1.145HisArg: 1.145 ± 0.294
0.992HisSer: 0.992 ± 0.255
0.687HisThr: 0.687 ± 0.208
1.45HisVal: 1.45 ± 0.347
0.305HisTrp: 0.305 ± 0.134
0.458HisTyr: 0.458 ± 0.16
0.0HisXaa: 0.0 ± 0.0
Ile
4.045IleAla: 4.045 ± 0.539
0.534IleCys: 0.534 ± 0.207
3.281IleAsp: 3.281 ± 0.658
3.281IleGlu: 3.281 ± 0.501
0.611IlePhe: 0.611 ± 0.201
3.129IleGly: 3.129 ± 0.525
1.068IleHis: 1.068 ± 0.266
1.45IleIle: 1.45 ± 0.374
2.137IleLys: 2.137 ± 0.291
2.595IleLeu: 2.595 ± 0.489
0.611IleMet: 0.611 ± 0.207
2.137IleAsn: 2.137 ± 0.3
2.595IlePro: 2.595 ± 0.498
1.526IleGln: 1.526 ± 0.244
3.053IleArg: 3.053 ± 0.482
2.442IleSer: 2.442 ± 0.355
3.51IleThr: 3.51 ± 0.838
3.129IleVal: 3.129 ± 0.558
0.229IleTrp: 0.229 ± 0.122
0.763IleTyr: 0.763 ± 0.183
0.0IleXaa: 0.0 ± 0.0
Lys
6.258LysAla: 6.258 ± 0.851
0.305LysCys: 0.305 ± 0.161
2.747LysAsp: 2.747 ± 0.531
3.129LysGlu: 3.129 ± 0.739
1.45LysPhe: 1.45 ± 0.347
4.655LysGly: 4.655 ± 0.519
1.679LysHis: 1.679 ± 0.448
1.755LysIle: 1.755 ± 0.375
1.603LysLys: 1.603 ± 0.372
4.35LysLeu: 4.35 ± 0.71
0.992LysMet: 0.992 ± 0.272
1.068LysAsn: 1.068 ± 0.254
2.213LysPro: 2.213 ± 0.476
2.137LysGln: 2.137 ± 0.427
3.434LysArg: 3.434 ± 0.442
2.595LysSer: 2.595 ± 0.38
2.366LysThr: 2.366 ± 0.466
3.129LysVal: 3.129 ± 0.519
0.916LysTrp: 0.916 ± 0.353
1.755LysTyr: 1.755 ± 0.41
0.0LysXaa: 0.0 ± 0.0
Leu
11.447LeuAla: 11.447 ± 0.985
0.992LeuCys: 0.992 ± 0.39
5.647LeuAsp: 5.647 ± 0.685
3.129LeuGlu: 3.129 ± 0.436
2.824LeuPhe: 2.824 ± 0.477
5.571LeuGly: 5.571 ± 0.515
2.213LeuHis: 2.213 ± 0.38
3.892LeuIle: 3.892 ± 0.662
4.655LeuLys: 4.655 ± 0.644
6.639LeuLeu: 6.639 ± 0.861
2.137LeuMet: 2.137 ± 0.348
3.51LeuAsn: 3.51 ± 0.542
3.892LeuPro: 3.892 ± 0.488
4.197LeuGln: 4.197 ± 0.496
6.258LeuArg: 6.258 ± 0.755
4.502LeuSer: 4.502 ± 0.575
6.258LeuThr: 6.258 ± 1.134
5.037LeuVal: 5.037 ± 0.637
1.603LeuTrp: 1.603 ± 0.276
2.747LeuTyr: 2.747 ± 0.445
0.0LeuXaa: 0.0 ± 0.0
Met
2.671MetAla: 2.671 ± 0.404
0.229MetCys: 0.229 ± 0.132
1.374MetAsp: 1.374 ± 0.433
1.603MetGlu: 1.603 ± 0.416
0.687MetPhe: 0.687 ± 0.247
1.45MetGly: 1.45 ± 0.275
0.687MetHis: 0.687 ± 0.194
0.763MetIle: 0.763 ± 0.242
0.611MetLys: 0.611 ± 0.239
2.06MetLeu: 2.06 ± 0.558
0.534MetMet: 0.534 ± 0.167
0.382MetAsn: 0.382 ± 0.15
0.839MetPro: 0.839 ± 0.235
1.374MetGln: 1.374 ± 0.388
1.908MetArg: 1.908 ± 0.385
1.908MetSer: 1.908 ± 0.415
1.221MetThr: 1.221 ± 0.257
1.374MetVal: 1.374 ± 0.286
0.305MetTrp: 0.305 ± 0.131
0.763MetTyr: 0.763 ± 0.18
0.0MetXaa: 0.0 ± 0.0
Asn
3.663AsnAla: 3.663 ± 0.508
0.229AsnCys: 0.229 ± 0.138
1.526AsnAsp: 1.526 ± 0.301
1.755AsnGlu: 1.755 ± 0.323
1.068AsnPhe: 1.068 ± 0.312
3.281AsnGly: 3.281 ± 0.58
0.382AsnHis: 0.382 ± 0.158
1.45AsnIle: 1.45 ± 0.403
1.603AsnLys: 1.603 ± 0.384
3.281AsnLeu: 3.281 ± 0.496
0.839AsnMet: 0.839 ± 0.216
1.068AsnAsn: 1.068 ± 0.274
1.908AsnPro: 1.908 ± 0.316
1.45AsnGln: 1.45 ± 0.286
1.297AsnArg: 1.297 ± 0.241
2.824AsnSer: 2.824 ± 0.552
3.205AsnThr: 3.205 ± 0.547
3.281AsnVal: 3.281 ± 0.495
0.763AsnTrp: 0.763 ± 0.275
0.992AsnTyr: 0.992 ± 0.259
0.0AsnXaa: 0.0 ± 0.0
Pro
5.495ProAla: 5.495 ± 0.71
1.221ProCys: 1.221 ± 0.698
2.442ProAsp: 2.442 ± 0.541
3.968ProGlu: 3.968 ± 0.593
1.374ProPhe: 1.374 ± 0.372
2.671ProGly: 2.671 ± 0.448
0.611ProHis: 0.611 ± 0.195
1.755ProIle: 1.755 ± 0.316
2.9ProLys: 2.9 ± 0.503
2.976ProLeu: 2.976 ± 0.539
0.687ProMet: 0.687 ± 0.227
1.832ProAsn: 1.832 ± 0.401
1.374ProPro: 1.374 ± 0.328
2.595ProGln: 2.595 ± 0.56
2.671ProArg: 2.671 ± 0.528
2.213ProSer: 2.213 ± 0.68
3.205ProThr: 3.205 ± 0.427
3.053ProVal: 3.053 ± 0.495
0.687ProTrp: 0.687 ± 0.217
1.374ProTyr: 1.374 ± 0.309
0.0ProXaa: 0.0 ± 0.0
Gln
5.647GlnAla: 5.647 ± 0.848
0.305GlnCys: 0.305 ± 0.138
2.137GlnAsp: 2.137 ± 0.469
3.129GlnGlu: 3.129 ± 0.424
0.916GlnPhe: 0.916 ± 0.234
4.884GlnGly: 4.884 ± 0.747
1.145GlnHis: 1.145 ± 0.313
1.755GlnIle: 1.755 ± 0.398
1.374GlnLys: 1.374 ± 0.307
3.739GlnLeu: 3.739 ± 0.607
1.068GlnMet: 1.068 ± 0.278
1.603GlnAsn: 1.603 ± 0.301
1.908GlnPro: 1.908 ± 0.536
2.976GlnGln: 2.976 ± 0.727
3.434GlnArg: 3.434 ± 0.633
2.442GlnSer: 2.442 ± 0.47
2.06GlnThr: 2.06 ± 0.397
2.747GlnVal: 2.747 ± 0.445
0.611GlnTrp: 0.611 ± 0.153
1.908GlnTyr: 1.908 ± 0.364
0.0GlnXaa: 0.0 ± 0.0
Arg
6.334ArgAla: 6.334 ± 0.737
1.221ArgCys: 1.221 ± 0.411
4.121ArgAsp: 4.121 ± 0.841
5.189ArgGlu: 5.189 ± 0.856
1.755ArgPhe: 1.755 ± 0.392
4.808ArgGly: 4.808 ± 0.625
1.45ArgHis: 1.45 ± 0.379
3.053ArgIle: 3.053 ± 0.478
3.434ArgLys: 3.434 ± 0.586
5.495ArgLeu: 5.495 ± 0.568
1.832ArgMet: 1.832 ± 0.429
1.603ArgAsn: 1.603 ± 0.297
2.06ArgPro: 2.06 ± 0.36
2.442ArgGln: 2.442 ± 0.476
4.502ArgArg: 4.502 ± 0.706
2.747ArgSer: 2.747 ± 0.4
3.129ArgThr: 3.129 ± 0.497
4.197ArgVal: 4.197 ± 0.767
0.992ArgTrp: 0.992 ± 0.317
1.297ArgTyr: 1.297 ± 0.319
0.0ArgXaa: 0.0 ± 0.0
Ser
6.41SerAla: 6.41 ± 0.94
0.611SerCys: 0.611 ± 0.216
3.129SerAsp: 3.129 ± 0.425
2.747SerGlu: 2.747 ± 0.511
1.755SerPhe: 1.755 ± 0.306
5.952SerGly: 5.952 ± 1.039
0.687SerHis: 0.687 ± 0.235
2.595SerIle: 2.595 ± 0.582
3.51SerLys: 3.51 ± 0.428
4.96SerLeu: 4.96 ± 0.58
1.145SerMet: 1.145 ± 0.248
2.137SerAsn: 2.137 ± 0.429
2.9SerPro: 2.9 ± 0.445
1.984SerGln: 1.984 ± 0.453
3.205SerArg: 3.205 ± 0.444
3.281SerSer: 3.281 ± 0.634
3.663SerThr: 3.663 ± 0.584
3.281SerVal: 3.281 ± 0.496
0.916SerTrp: 0.916 ± 0.214
1.221SerTyr: 1.221 ± 0.305
0.0SerXaa: 0.0 ± 0.0
Thr
8.242ThrAla: 8.242 ± 2.209
0.382ThrCys: 0.382 ± 0.188
3.816ThrAsp: 3.816 ± 0.623
3.129ThrGlu: 3.129 ± 0.426
1.755ThrPhe: 1.755 ± 0.346
6.334ThrGly: 6.334 ± 1.835
1.068ThrHis: 1.068 ± 0.257
2.442ThrIle: 2.442 ± 0.639
1.755ThrLys: 1.755 ± 0.348
5.647ThrLeu: 5.647 ± 1.179
1.679ThrMet: 1.679 ± 0.368
1.908ThrAsn: 1.908 ± 0.431
3.358ThrPro: 3.358 ± 0.463
2.137ThrGln: 2.137 ± 0.494
3.434ThrArg: 3.434 ± 0.579
4.35ThrSer: 4.35 ± 0.913
3.968ThrThr: 3.968 ± 1.077
5.037ThrVal: 5.037 ± 0.816
1.374ThrTrp: 1.374 ± 0.235
2.06ThrTyr: 2.06 ± 0.447
0.0ThrXaa: 0.0 ± 0.0
Val
7.25ValAla: 7.25 ± 0.908
0.382ValCys: 0.382 ± 0.199
3.205ValAsp: 3.205 ± 0.359
3.816ValGlu: 3.816 ± 0.617
1.908ValPhe: 1.908 ± 0.384
5.266ValGly: 5.266 ± 0.636
1.755ValHis: 1.755 ± 0.311
2.595ValIle: 2.595 ± 0.294
4.121ValLys: 4.121 ± 0.588
6.563ValLeu: 6.563 ± 0.794
0.992ValMet: 0.992 ± 0.241
2.671ValAsn: 2.671 ± 0.51
3.358ValPro: 3.358 ± 0.546
3.968ValGln: 3.968 ± 0.593
3.968ValArg: 3.968 ± 0.859
3.816ValSer: 3.816 ± 0.915
4.884ValThr: 4.884 ± 0.868
4.426ValVal: 4.426 ± 0.783
1.068ValTrp: 1.068 ± 0.269
1.45ValTyr: 1.45 ± 0.343
0.0ValXaa: 0.0 ± 0.0
Trp
1.679TrpAla: 1.679 ± 0.408
0.229TrpCys: 0.229 ± 0.156
0.839TrpAsp: 0.839 ± 0.271
0.611TrpGlu: 0.611 ± 0.239
0.687TrpPhe: 0.687 ± 0.205
1.145TrpGly: 1.145 ± 0.431
0.382TrpHis: 0.382 ± 0.158
0.153TrpIle: 0.153 ± 0.123
0.611TrpLys: 0.611 ± 0.231
1.832TrpLeu: 1.832 ± 0.518
0.611TrpMet: 0.611 ± 0.23
0.611TrpAsn: 0.611 ± 0.175
0.153TrpPro: 0.153 ± 0.103
0.992TrpGln: 0.992 ± 0.249
1.145TrpArg: 1.145 ± 0.252
0.763TrpSer: 0.763 ± 0.238
0.839TrpThr: 0.839 ± 0.251
1.526TrpVal: 1.526 ± 0.425
0.305TrpTrp: 0.305 ± 0.142
0.611TrpTyr: 0.611 ± 0.198
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.9TyrAla: 2.9 ± 0.594
0.229TyrCys: 0.229 ± 0.113
1.068TyrAsp: 1.068 ± 0.291
1.908TyrGlu: 1.908 ± 0.39
0.763TyrPhe: 0.763 ± 0.237
2.595TyrGly: 2.595 ± 0.44
0.382TyrHis: 0.382 ± 0.157
1.068TyrIle: 1.068 ± 0.268
1.374TyrLys: 1.374 ± 0.278
2.595TyrLeu: 2.595 ± 0.493
0.763TyrMet: 0.763 ± 0.229
1.832TyrAsn: 1.832 ± 0.354
1.603TyrPro: 1.603 ± 0.365
1.526TyrGln: 1.526 ± 0.299
1.908TyrArg: 1.908 ± 0.335
1.45TyrSer: 1.45 ± 0.25
1.603TyrThr: 1.603 ± 0.427
1.45TyrVal: 1.45 ± 0.34
0.229TyrTrp: 0.229 ± 0.147
0.839TyrTyr: 0.839 ± 0.206
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (13105 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski