Amino acid dipepetide frequency for alpha proteobacterium BAL199

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.491AlaAla: 17.491 ± 0.157	1.134AlaCys: 1.134 ± 0.026	7.709AlaAsp: 7.709 ± 0.072	7.762AlaGlu: 7.762 ± 0.079	4.322AlaPhe: 4.322 ± 0.048	11.327AlaGly: 11.327 ± 0.092	2.281AlaHis: 2.281 ± 0.037	6.234AlaIle: 6.234 ± 0.06	3.466AlaLys: 3.466 ± 0.061	13.463AlaLeu: 13.463 ± 0.116	3.463AlaMet: 3.463 ± 0.045	2.644AlaAsn: 2.644 ± 0.047	5.389AlaPro: 5.389 ± 0.062	3.252AlaGln: 3.252 ± 0.05	9.012AlaArg: 9.012 ± 0.089	6.061AlaSer: 6.061 ± 0.068	6.394AlaThr: 6.394 ± 0.06	10.362AlaVal: 10.362 ± 0.091	1.728AlaTrp: 1.728 ± 0.034	2.536AlaTyr: 2.536 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	0.978CysAla: 0.978 ± 0.022	0.137CysCys: 0.137 ± 0.009	0.568CysAsp: 0.568 ± 0.02	0.385CysGlu: 0.385 ± 0.016	0.339CysPhe: 0.339 ± 0.014	0.951CysGly: 0.951 ± 0.027	0.258CysHis: 0.258 ± 0.012	0.362CysIle: 0.362 ± 0.015	0.162CysLys: 0.162 ± 0.01	0.846CysLeu: 0.846 ± 0.026	0.157CysMet: 0.157 ± 0.01	0.195CysAsn: 0.195 ± 0.012	0.482CysPro: 0.482 ± 0.016	0.225CysGln: 0.225 ± 0.012	0.831CysArg: 0.831 ± 0.022	0.427CysSer: 0.427 ± 0.018	0.451CysThr: 0.451 ± 0.016	0.659CysVal: 0.659 ± 0.02	0.146CysTrp: 0.146 ± 0.01	0.206CysTyr: 0.206 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.691AspAla: 7.691 ± 0.081	0.481AspCys: 0.481 ± 0.017	3.779AspAsp: 3.779 ± 0.06	3.328AspGlu: 3.328 ± 0.043	1.968AspPhe: 1.968 ± 0.031	6.365AspGly: 6.365 ± 0.072	1.319AspHis: 1.319 ± 0.033	2.636AspIle: 2.636 ± 0.04	1.398AspLys: 1.398 ± 0.036	6.639AspLeu: 6.639 ± 0.078	1.228AspMet: 1.228 ± 0.025	1.089AspAsn: 1.089 ± 0.025	4.259AspPro: 4.259 ± 0.051	1.752AspGln: 1.752 ± 0.033	5.648AspArg: 5.648 ± 0.069	2.457AspSer: 2.457 ± 0.036	2.966AspThr: 2.966 ± 0.052	4.506AspVal: 4.506 ± 0.045	1.088AspTrp: 1.088 ± 0.028	1.394AspTyr: 1.394 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	7.606GluAla: 7.606 ± 0.074	0.348GluCys: 0.348 ± 0.014	2.554GluAsp: 2.554 ± 0.041	2.572GluGlu: 2.572 ± 0.044	1.68GluPhe: 1.68 ± 0.027	3.844GluGly: 3.844 ± 0.053	1.307GluHis: 1.307 ± 0.026	3.188GluIle: 3.188 ± 0.047	1.522GluLys: 1.522 ± 0.034	5.152GluLeu: 5.152 ± 0.054	1.365GluMet: 1.365 ± 0.034	1.228GluAsn: 1.228 ± 0.026	2.97GluPro: 2.97 ± 0.048	1.944GluGln: 1.944 ± 0.037	5.275GluArg: 5.275 ± 0.056	2.362GluSer: 2.362 ± 0.035	3.4GluThr: 3.4 ± 0.04	4.009GluVal: 4.009 ± 0.045	0.718GluTrp: 0.718 ± 0.021	0.989GluTyr: 0.989 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	4.289PheAla: 4.289 ± 0.052	0.38PheCys: 0.38 ± 0.014	2.683PheAsp: 2.683 ± 0.038	2.138PheGlu: 2.138 ± 0.037	1.213PhePhe: 1.213 ± 0.029	3.595PheGly: 3.595 ± 0.047	0.78PheHis: 0.78 ± 0.023	1.319PheIle: 1.319 ± 0.029	0.902PheLys: 0.902 ± 0.024	3.135PheLeu: 3.135 ± 0.044	0.751PheMet: 0.751 ± 0.02	0.894PheAsn: 0.894 ± 0.022	1.557PhePro: 1.557 ± 0.026	0.993PheGln: 0.993 ± 0.025	2.384PheArg: 2.384 ± 0.038	1.733PheSer: 1.733 ± 0.03	1.864PheThr: 1.864 ± 0.033	2.76PheVal: 2.76 ± 0.042	0.51PheTrp: 0.51 ± 0.019	0.81PheTyr: 0.81 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	9.25GlyAla: 9.25 ± 0.096	0.931GlyCys: 0.931 ± 0.026	4.83GlyAsp: 4.83 ± 0.063	4.297GlyGlu: 4.297 ± 0.044	3.62GlyPhe: 3.62 ± 0.047	7.615GlyGly: 7.615 ± 0.091	2.024GlyHis: 2.024 ± 0.035	4.411GlyIle: 4.411 ± 0.047	2.703GlyLys: 2.703 ± 0.044	9.236GlyLeu: 9.236 ± 0.082	2.392GlyMet: 2.392 ± 0.04	2.047GlyAsn: 2.047 ± 0.058	3.933GlyPro: 3.933 ± 0.048	2.552GlyGln: 2.552 ± 0.041	7.015GlyArg: 7.015 ± 0.07	4.853GlySer: 4.853 ± 0.061	4.909GlyThr: 4.909 ± 0.057	6.879GlyVal: 6.879 ± 0.069	1.524GlyTrp: 1.524 ± 0.031	2.383GlyTyr: 2.383 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	2.448HisAla: 2.448 ± 0.036	0.259HisCys: 0.259 ± 0.013	1.316HisAsp: 1.316 ± 0.029	1.024HisGlu: 1.024 ± 0.025	0.733HisPhe: 0.733 ± 0.02	2.106HisGly: 2.106 ± 0.038	0.618HisHis: 0.618 ± 0.023	0.814HisIle: 0.814 ± 0.022	0.446HisLys: 0.446 ± 0.017	2.196HisLeu: 2.196 ± 0.033	0.452HisMet: 0.452 ± 0.015	0.452HisAsn: 0.452 ± 0.018	1.52HisPro: 1.52 ± 0.031	0.62HisGln: 0.62 ± 0.018	1.827HisArg: 1.827 ± 0.037	0.968HisSer: 0.968 ± 0.025	0.944HisThr: 0.944 ± 0.021	1.564HisVal: 1.564 ± 0.029	0.347HisTrp: 0.347 ± 0.013	0.519HisTyr: 0.519 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	6.955IleAla: 6.955 ± 0.068	0.497IleCys: 0.497 ± 0.018	3.801IleAsp: 3.801 ± 0.053	3.419IleGlu: 3.419 ± 0.05	1.223IlePhe: 1.223 ± 0.029	5.021IleGly: 5.021 ± 0.057	0.934IleHis: 0.934 ± 0.024	1.743IleIle: 1.743 ± 0.035	1.252IleLys: 1.252 ± 0.03	4.203IleLeu: 4.203 ± 0.051	0.85IleMet: 0.85 ± 0.021	1.227IleAsn: 1.227 ± 0.027	2.338IlePro: 2.338 ± 0.039	1.214IleGln: 1.214 ± 0.028	3.469IleArg: 3.469 ± 0.049	2.317IleSer: 2.317 ± 0.043	2.496IleThr: 2.496 ± 0.04	4.111IleVal: 4.111 ± 0.048	0.589IleTrp: 0.589 ± 0.016	0.958IleTyr: 0.958 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	3.643LysAla: 3.643 ± 0.062	0.127LysCys: 0.127 ± 0.008	1.435LysAsp: 1.435 ± 0.031	1.165LysGlu: 1.165 ± 0.03	0.738LysPhe: 0.738 ± 0.023	2.193LysGly: 2.193 ± 0.04	0.529LysHis: 0.529 ± 0.017	1.389LysIle: 1.389 ± 0.03	0.873LysLys: 0.873 ± 0.026	2.754LysLeu: 2.754 ± 0.046	0.588LysMet: 0.588 ± 0.02	0.624LysAsn: 0.624 ± 0.019	1.745LysPro: 1.745 ± 0.032	0.796LysGln: 0.796 ± 0.023	2.249LysArg: 2.249 ± 0.041	1.484LysSer: 1.484 ± 0.029	1.588LysThr: 1.588 ± 0.03	2.203LysVal: 2.203 ± 0.04	0.308LysTrp: 0.308 ± 0.014	0.496LysTyr: 0.496 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	13.866LeuAla: 13.866 ± 0.104	0.877LeuCys: 0.877 ± 0.024	6.868LeuAsp: 6.868 ± 0.058	5.345LeuGlu: 5.345 ± 0.059	3.448LeuPhe: 3.448 ± 0.049	8.549LeuGly: 8.549 ± 0.076	1.976LeuHis: 1.976 ± 0.032	4.816LeuIle: 4.816 ± 0.065	2.974LeuLys: 2.974 ± 0.045	9.209LeuLeu: 9.209 ± 0.093	2.413LeuMet: 2.413 ± 0.038	2.376LeuAsn: 2.376 ± 0.037	5.557LeuPro: 5.557 ± 0.06	2.618LeuGln: 2.618 ± 0.043	7.241LeuArg: 7.241 ± 0.08	6.021LeuSer: 6.021 ± 0.068	5.697LeuThr: 5.697 ± 0.06	7.786LeuVal: 7.786 ± 0.077	1.238LeuTrp: 1.238 ± 0.028	2.034LeuTyr: 2.034 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	3.238MetAla: 3.238 ± 0.047	0.159MetCys: 0.159 ± 0.008	1.182MetAsp: 1.182 ± 0.03	1.013MetGlu: 1.013 ± 0.028	0.69MetPhe: 0.69 ± 0.024	1.68MetGly: 1.68 ± 0.037	0.414MetHis: 0.414 ± 0.017	1.346MetIle: 1.346 ± 0.025	0.81MetLys: 0.81 ± 0.023	2.392MetLeu: 2.392 ± 0.039	0.635MetMet: 0.635 ± 0.02	0.664MetAsn: 0.664 ± 0.018	1.517MetPro: 1.517 ± 0.03	0.706MetGln: 0.706 ± 0.02	1.804MetArg: 1.804 ± 0.033	1.505MetSer: 1.505 ± 0.029	2.03MetThr: 2.03 ± 0.031	1.944MetVal: 1.944 ± 0.035	0.225MetTrp: 0.225 ± 0.011	0.334MetTyr: 0.334 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.858AsnAla: 2.858 ± 0.046	0.196AsnCys: 0.196 ± 0.009	1.404AsnAsp: 1.404 ± 0.048	1.0AsnGlu: 1.0 ± 0.024	0.75AsnPhe: 0.75 ± 0.021	2.127AsnGly: 2.127 ± 0.037	0.515AsnHis: 0.515 ± 0.017	1.051AsnIle: 1.051 ± 0.027	0.517AsnLys: 0.517 ± 0.016	2.496AsnLeu: 2.496 ± 0.041	0.479AsnMet: 0.479 ± 0.016	0.569AsnAsn: 0.569 ± 0.019	1.691AsnPro: 1.691 ± 0.034	0.689AsnGln: 0.689 ± 0.02	1.827AsnArg: 1.827 ± 0.032	1.012AsnSer: 1.012 ± 0.023	1.15AsnThr: 1.15 ± 0.029	1.752AsnVal: 1.752 ± 0.032	0.367AsnTrp: 0.367 ± 0.016	0.54AsnTyr: 0.54 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	6.528ProAla: 6.528 ± 0.073	0.334ProCys: 0.334 ± 0.015	4.025ProAsp: 4.025 ± 0.053	3.576ProGlu: 3.576 ± 0.046	1.888ProPhe: 1.888 ± 0.032	4.703ProGly: 4.703 ± 0.056	1.066ProHis: 1.066 ± 0.026	2.587ProIle: 2.587 ± 0.043	1.548ProLys: 1.548 ± 0.034	4.688ProLeu: 4.688 ± 0.052	1.366ProMet: 1.366 ± 0.026	1.366ProAsn: 1.366 ± 0.032	2.745ProPro: 2.745 ± 0.061	1.344ProGln: 1.344 ± 0.024	3.128ProArg: 3.128 ± 0.044	3.06ProSer: 3.06 ± 0.048	3.114ProThr: 3.114 ± 0.046	4.69ProVal: 4.69 ± 0.056	0.828ProTrp: 0.828 ± 0.023	1.165ProTyr: 1.165 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	3.793GlnAla: 3.793 ± 0.045	0.19GlnCys: 0.19 ± 0.01	1.387GlnAsp: 1.387 ± 0.028	1.286GlnGlu: 1.286 ± 0.03	0.919GlnPhe: 0.919 ± 0.022	2.096GlnGly: 2.096 ± 0.037	0.631GlnHis: 0.631 ± 0.019	1.693GlnIle: 1.693 ± 0.044	0.794GlnLys: 0.794 ± 0.023	2.446GlnLeu: 2.446 ± 0.037	0.767GlnMet: 0.767 ± 0.021	0.693GlnAsn: 0.693 ± 0.022	1.668GlnPro: 1.668 ± 0.035	0.938GlnGln: 0.938 ± 0.027	2.513GlnArg: 2.513 ± 0.04	1.58GlnSer: 1.58 ± 0.028	1.602GlnThr: 1.602 ± 0.033	2.263GlnVal: 2.263 ± 0.045	0.358GlnTrp: 0.358 ± 0.012	0.532GlnTyr: 0.532 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	8.375ArgAla: 8.375 ± 0.08	0.702ArgCys: 0.702 ± 0.022	4.558ArgAsp: 4.558 ± 0.058	3.771ArgGlu: 3.771 ± 0.048	3.097ArgPhe: 3.097 ± 0.037	5.164ArgGly: 5.164 ± 0.055	2.01ArgHis: 2.01 ± 0.04	4.258ArgIle: 4.258 ± 0.053	1.986ArgLys: 1.986 ± 0.035	8.912ArgLeu: 8.912 ± 0.084	2.024ArgMet: 2.024 ± 0.034	1.75ArgAsn: 1.75 ± 0.035	4.268ArgPro: 4.268 ± 0.057	2.565ArgGln: 2.565 ± 0.044	7.207ArgArg: 7.207 ± 0.072	4.214ArgSer: 4.214 ± 0.051	3.988ArgThr: 3.988 ± 0.052	5.641ArgVal: 5.641 ± 0.056	1.272ArgTrp: 1.272 ± 0.026	1.906ArgTyr: 1.906 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	6.254SerAla: 6.254 ± 0.064	0.435SerCys: 0.435 ± 0.017	3.165SerAsp: 3.165 ± 0.041	2.605SerGlu: 2.605 ± 0.041	1.951SerPhe: 1.951 ± 0.032	5.316SerGly: 5.316 ± 0.06	1.031SerHis: 1.031 ± 0.024	2.594SerIle: 2.594 ± 0.034	1.391SerLys: 1.391 ± 0.031	4.897SerLeu: 4.897 ± 0.059	1.361SerMet: 1.361 ± 0.026	1.204SerAsn: 1.204 ± 0.027	2.685SerPro: 2.685 ± 0.04	1.349SerGln: 1.349 ± 0.031	3.562SerArg: 3.562 ± 0.05	2.819SerSer: 2.819 ± 0.046	2.766SerThr: 2.766 ± 0.043	4.291SerVal: 4.291 ± 0.05	0.739SerTrp: 0.739 ± 0.021	1.173SerTyr: 1.173 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	6.816ThrAla: 6.816 ± 0.068	0.439ThrCys: 0.439 ± 0.017	3.247ThrAsp: 3.247 ± 0.044	2.71ThrGlu: 2.71 ± 0.042	1.872ThrPhe: 1.872 ± 0.035	5.341ThrGly: 5.341 ± 0.06	1.024ThrHis: 1.024 ± 0.025	2.783ThrIle: 2.783 ± 0.048	1.295ThrLys: 1.295 ± 0.033	6.082ThrLeu: 6.082 ± 0.063	1.292ThrMet: 1.292 ± 0.028	1.173ThrAsn: 1.173 ± 0.028	3.302ThrPro: 3.302 ± 0.046	1.296ThrGln: 1.296 ± 0.029	3.652ThrArg: 3.652 ± 0.044	2.6ThrSer: 2.6 ± 0.042	3.036ThrThr: 3.036 ± 0.044	5.279ThrVal: 5.279 ± 0.055	0.703ThrTrp: 0.703 ± 0.021	1.115ThrTyr: 1.115 ± 0.025	0.0ThrXaa: 0.0 ± 0.0
Val	10.089ValAla: 10.089 ± 0.09	0.74ValCys: 0.74 ± 0.02	5.316ValAsp: 5.316 ± 0.064	4.912ValGlu: 4.912 ± 0.05	2.913ValPhe: 2.913 ± 0.043	6.394ValGly: 6.394 ± 0.067	1.587ValHis: 1.587 ± 0.03	3.871ValIle: 3.871 ± 0.052	1.957ValLys: 1.957 ± 0.034	8.192ValLeu: 8.192 ± 0.082	1.899ValMet: 1.899 ± 0.035	1.851ValAsn: 1.851 ± 0.035	4.229ValPro: 4.229 ± 0.05	2.077ValGln: 2.077 ± 0.037	5.635ValArg: 5.635 ± 0.054	4.225ValSer: 4.225 ± 0.052	4.707ValThr: 4.707 ± 0.051	6.981ValVal: 6.981 ± 0.068	1.041ValTrp: 1.041 ± 0.023	1.569ValTyr: 1.569 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.319TrpAla: 1.319 ± 0.033	0.171TrpCys: 0.171 ± 0.01	0.714TrpAsp: 0.714 ± 0.021	0.582TrpGlu: 0.582 ± 0.017	0.544TrpPhe: 0.544 ± 0.018	0.963TrpGly: 0.963 ± 0.022	0.379TrpHis: 0.379 ± 0.014	0.743TrpIle: 0.743 ± 0.023	0.402TrpLys: 0.402 ± 0.016	1.697TrpLeu: 1.697 ± 0.032	0.398TrpMet: 0.398 ± 0.014	0.412TrpAsn: 0.412 ± 0.015	0.779TrpPro: 0.779 ± 0.022	0.589TrpGln: 0.589 ± 0.019	1.411TrpArg: 1.411 ± 0.031	0.866TrpSer: 0.866 ± 0.022	0.865TrpThr: 0.865 ± 0.022	0.919TrpVal: 0.919 ± 0.024	0.231TrpTrp: 0.231 ± 0.011	0.309TrpTyr: 0.309 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.542TyrAla: 2.542 ± 0.039	0.227TyrCys: 0.227 ± 0.013	1.448TyrAsp: 1.448 ± 0.029	1.143TyrGlu: 1.143 ± 0.028	0.785TyrPhe: 0.785 ± 0.024	2.053TyrGly: 2.053 ± 0.043	0.492TyrHis: 0.492 ± 0.018	0.778TyrIle: 0.778 ± 0.023	0.53TyrLys: 0.53 ± 0.02	2.271TyrLeu: 2.271 ± 0.035	0.408TyrMet: 0.408 ± 0.017	0.532TyrAsn: 0.532 ± 0.019	1.116TyrPro: 1.116 ± 0.025	0.629TyrGln: 0.629 ± 0.02	1.978TyrArg: 1.978 ± 0.039	1.024TyrSer: 1.024 ± 0.023	1.091TyrThr: 1.091 ± 0.028	1.587TyrVal: 1.587 ± 0.031	0.336TyrTrp: 0.336 ± 0.012	0.522TyrTyr: 0.522 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5881 proteins (1761557 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---