Amino acid dipepetide frequency for Bacillus sp. SG-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.21AlaAla: 6.21 ± 0.113	0.513AlaCys: 0.513 ± 0.025	3.482AlaAsp: 3.482 ± 0.069	5.164AlaGlu: 5.164 ± 0.083	3.254AlaPhe: 3.254 ± 0.061	5.77AlaGly: 5.77 ± 0.083	1.21AlaHis: 1.21 ± 0.041	5.092AlaIle: 5.092 ± 0.078	4.367AlaLys: 4.367 ± 0.075	6.97AlaLeu: 6.97 ± 0.103	2.009AlaMet: 2.009 ± 0.042	2.469AlaAsn: 2.469 ± 0.047	1.988AlaPro: 1.988 ± 0.051	2.157AlaGln: 2.157 ± 0.047	2.529AlaArg: 2.529 ± 0.043	3.954AlaSer: 3.954 ± 0.066	2.786AlaThr: 2.786 ± 0.053	5.709AlaVal: 5.709 ± 0.084	0.596AlaTrp: 0.596 ± 0.025	2.23AlaTyr: 2.23 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.377CysAla: 0.377 ± 0.021	0.106CysCys: 0.106 ± 0.01	0.392CysAsp: 0.392 ± 0.022	0.417CysGlu: 0.417 ± 0.02	0.333CysPhe: 0.333 ± 0.015	0.636CysGly: 0.636 ± 0.023	0.178CysHis: 0.178 ± 0.015	0.501CysIle: 0.501 ± 0.023	0.323CysLys: 0.323 ± 0.017	0.686CysLeu: 0.686 ± 0.027	0.172CysMet: 0.172 ± 0.013	0.272CysAsn: 0.272 ± 0.016	0.337CysPro: 0.337 ± 0.02	0.205CysGln: 0.205 ± 0.014	0.292CysArg: 0.292 ± 0.018	0.488CysSer: 0.488 ± 0.024	0.367CysThr: 0.367 ± 0.019	0.362CysVal: 0.362 ± 0.021	0.076CysTrp: 0.076 ± 0.008	0.251CysTyr: 0.251 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	3.166AspAla: 3.166 ± 0.059	0.386AspCys: 0.386 ± 0.02	2.429AspAsp: 2.429 ± 0.058	4.655AspGlu: 4.655 ± 0.075	2.506AspPhe: 2.506 ± 0.055	3.39AspGly: 3.39 ± 0.06	1.142AspHis: 1.142 ± 0.038	4.281AspIle: 4.281 ± 0.064	3.233AspLys: 3.233 ± 0.062	5.139AspLeu: 5.139 ± 0.096	1.471AspMet: 1.471 ± 0.042	1.895AspAsn: 1.895 ± 0.049	1.957AspPro: 1.957 ± 0.05	1.824AspGln: 1.824 ± 0.041	2.34AspArg: 2.34 ± 0.051	3.018AspSer: 3.018 ± 0.057	2.492AspThr: 2.492 ± 0.05	3.49AspVal: 3.49 ± 0.062	0.651AspTrp: 0.651 ± 0.025	2.165AspTyr: 2.165 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	5.533GluAla: 5.533 ± 0.077	0.401GluCys: 0.401 ± 0.019	4.374GluAsp: 4.374 ± 0.071	8.591GluGlu: 8.591 ± 0.13	2.953GluPhe: 2.953 ± 0.052	5.108GluGly: 5.108 ± 0.078	1.434GluHis: 1.434 ± 0.035	5.705GluIle: 5.705 ± 0.082	7.38GluLys: 7.38 ± 0.09	7.217GluLeu: 7.217 ± 0.103	2.546GluMet: 2.546 ± 0.048	4.005GluAsn: 4.005 ± 0.063	1.991GluPro: 1.991 ± 0.049	2.869GluGln: 2.869 ± 0.057	3.615GluArg: 3.615 ± 0.071	3.969GluSer: 3.969 ± 0.068	4.139GluThr: 4.139 ± 0.067	5.529GluVal: 5.529 ± 0.074	0.896GluTrp: 0.896 ± 0.029	2.398GluTyr: 2.398 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	2.952PheAla: 2.952 ± 0.055	0.348PheCys: 0.348 ± 0.019	2.323PheAsp: 2.323 ± 0.047	2.962PheGlu: 2.962 ± 0.055	2.563PhePhe: 2.563 ± 0.056	3.272PheGly: 3.272 ± 0.066	1.1PheHis: 1.1 ± 0.033	3.966PheIle: 3.966 ± 0.076	2.518PheLys: 2.518 ± 0.051	4.913PheLeu: 4.913 ± 0.084	1.267PheMet: 1.267 ± 0.031	1.969PheAsn: 1.969 ± 0.037	1.781PhePro: 1.781 ± 0.043	1.71PheGln: 1.71 ± 0.042	1.644PheArg: 1.644 ± 0.048	3.562PheSer: 3.562 ± 0.065	2.673PheThr: 2.673 ± 0.057	2.975PheVal: 2.975 ± 0.058	0.53PheTrp: 0.53 ± 0.023	1.842PheTyr: 1.842 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	4.85GlyAla: 4.85 ± 0.079	0.624GlyCys: 0.624 ± 0.023	3.367GlyAsp: 3.367 ± 0.066	5.032GlyGlu: 5.032 ± 0.086	3.533GlyPhe: 3.533 ± 0.057	5.103GlyGly: 5.103 ± 0.084	1.368GlyHis: 1.368 ± 0.035	5.964GlyIle: 5.964 ± 0.082	5.196GlyLys: 5.196 ± 0.068	6.788GlyLeu: 6.788 ± 0.089	2.259GlyMet: 2.259 ± 0.048	2.852GlyAsn: 2.852 ± 0.05	1.692GlyPro: 1.692 ± 0.039	2.095GlyGln: 2.095 ± 0.05	2.871GlyArg: 2.871 ± 0.06	4.281GlySer: 4.281 ± 0.059	4.074GlyThr: 4.074 ± 0.072	5.053GlyVal: 5.053 ± 0.078	0.831GlyTrp: 0.831 ± 0.029	2.741GlyTyr: 2.741 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	1.17HisAla: 1.17 ± 0.034	0.185HisCys: 0.185 ± 0.013	0.951HisAsp: 0.951 ± 0.032	1.454HisGlu: 1.454 ± 0.039	1.13HisPhe: 1.13 ± 0.031	1.409HisGly: 1.409 ± 0.038	0.642HisHis: 0.642 ± 0.029	1.418HisIle: 1.418 ± 0.034	1.061HisLys: 1.061 ± 0.032	2.092HisLeu: 2.092 ± 0.046	0.486HisMet: 0.486 ± 0.023	0.797HisAsn: 0.797 ± 0.028	1.105HisPro: 1.105 ± 0.031	0.786HisGln: 0.786 ± 0.029	0.887HisArg: 0.887 ± 0.03	1.389HisSer: 1.389 ± 0.035	1.023HisThr: 1.023 ± 0.035	1.213HisVal: 1.213 ± 0.031	0.227HisTrp: 0.227 ± 0.014	0.904HisTyr: 0.904 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.364IleAla: 5.364 ± 0.082	0.523IleCys: 0.523 ± 0.021	4.207IleAsp: 4.207 ± 0.068	5.659IleGlu: 5.659 ± 0.078	3.295IlePhe: 3.295 ± 0.063	5.757IleGly: 5.757 ± 0.086	1.637IleHis: 1.637 ± 0.046	5.732IleIle: 5.732 ± 0.083	4.567IleLys: 4.567 ± 0.071	7.418IleLeu: 7.418 ± 0.111	1.815IleMet: 1.815 ± 0.046	3.172IleAsn: 3.172 ± 0.058	3.23IlePro: 3.23 ± 0.063	2.728IleGln: 2.728 ± 0.055	2.944IleArg: 2.944 ± 0.063	5.202IleSer: 5.202 ± 0.08	4.111IleThr: 4.111 ± 0.065	4.963IleVal: 4.963 ± 0.079	0.608IleTrp: 0.608 ± 0.025	2.348IleTyr: 2.348 ± 0.048	0.0IleXaa: 0.0 ± 0.0
Lys	4.885LysAla: 4.885 ± 0.074	0.357LysCys: 0.357 ± 0.019	3.965LysAsp: 3.965 ± 0.064	7.317LysGlu: 7.317 ± 0.097	2.056LysPhe: 2.056 ± 0.047	4.911LysGly: 4.911 ± 0.079	1.285LysHis: 1.285 ± 0.038	4.426LysIle: 4.426 ± 0.071	5.927LysLys: 5.927 ± 0.09	5.85LysLeu: 5.85 ± 0.082	2.274LysMet: 2.274 ± 0.047	3.2LysAsn: 3.2 ± 0.061	2.205LysPro: 2.205 ± 0.05	2.773LysGln: 2.773 ± 0.055	3.336LysArg: 3.336 ± 0.061	3.732LysSer: 3.732 ± 0.06	3.496LysThr: 3.496 ± 0.055	4.75LysVal: 4.75 ± 0.075	0.85LysTrp: 0.85 ± 0.031	2.093LysTyr: 2.093 ± 0.043	0.0LysXaa: 0.0 ± 0.0
Leu	7.104LeuAla: 7.104 ± 0.082	0.574LeuCys: 0.574 ± 0.026	4.681LeuAsp: 4.681 ± 0.074	6.922LeuGlu: 6.922 ± 0.094	5.165LeuPhe: 5.165 ± 0.095	6.283LeuGly: 6.283 ± 0.091	1.92LeuHis: 1.92 ± 0.045	7.242LeuIle: 7.242 ± 0.106	6.974LeuLys: 6.974 ± 0.083	10.345LeuLeu: 10.345 ± 0.157	2.659LeuMet: 2.659 ± 0.055	4.253LeuAsn: 4.253 ± 0.07	3.954LeuPro: 3.954 ± 0.067	3.69LeuGln: 3.69 ± 0.064	3.579LeuArg: 3.579 ± 0.063	6.969LeuSer: 6.969 ± 0.085	5.627LeuThr: 5.627 ± 0.066	6.095LeuVal: 6.095 ± 0.078	0.847LeuTrp: 0.847 ± 0.029	3.201LeuTyr: 3.201 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	2.078MetAla: 2.078 ± 0.043	0.137MetCys: 0.137 ± 0.013	1.568MetAsp: 1.568 ± 0.043	2.326MetGlu: 2.326 ± 0.049	1.125MetPhe: 1.125 ± 0.034	1.908MetGly: 1.908 ± 0.045	0.427MetHis: 0.427 ± 0.022	2.211MetIle: 2.211 ± 0.053	2.714MetLys: 2.714 ± 0.046	2.575MetLeu: 2.575 ± 0.055	0.966MetMet: 0.966 ± 0.037	1.66MetAsn: 1.66 ± 0.043	1.016MetPro: 1.016 ± 0.037	0.83MetGln: 0.83 ± 0.031	1.121MetArg: 1.121 ± 0.035	1.649MetSer: 1.649 ± 0.035	1.672MetThr: 1.672 ± 0.043	1.845MetVal: 1.845 ± 0.044	0.21MetTrp: 0.21 ± 0.014	0.751MetTyr: 0.751 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	2.582AsnAla: 2.582 ± 0.05	0.294AsnCys: 0.294 ± 0.017	2.287AsnAsp: 2.287 ± 0.041	3.698AsnGlu: 3.698 ± 0.058	1.693AsnPhe: 1.693 ± 0.043	3.294AsnGly: 3.294 ± 0.061	1.023AsnHis: 1.023 ± 0.03	3.175AsnIle: 3.175 ± 0.057	2.859AsnLys: 2.859 ± 0.052	3.844AsnLeu: 3.844 ± 0.066	1.209AsnMet: 1.209 ± 0.038	1.969AsnAsn: 1.969 ± 0.05	2.154AsnPro: 2.154 ± 0.05	1.739AsnGln: 1.739 ± 0.04	2.009AsnArg: 2.009 ± 0.046	2.461AsnSer: 2.461 ± 0.054	2.114AsnThr: 2.114 ± 0.051	2.83AsnVal: 2.83 ± 0.046	0.504AsnTrp: 0.504 ± 0.022	1.458AsnTyr: 1.458 ± 0.039	0.0AsnXaa: 0.0 ± 0.0
Pro	2.449ProAla: 2.449 ± 0.057	0.239ProCys: 0.239 ± 0.014	2.161ProAsp: 2.161 ± 0.049	3.24ProGlu: 3.24 ± 0.051	2.017ProPhe: 2.017 ± 0.045	2.433ProGly: 2.433 ± 0.057	0.771ProHis: 0.771 ± 0.03	2.393ProIle: 2.393 ± 0.046	1.99ProLys: 1.99 ± 0.042	3.538ProLeu: 3.538 ± 0.068	0.85ProMet: 0.85 ± 0.034	1.446ProAsn: 1.446 ± 0.038	1.09ProPro: 1.09 ± 0.031	1.249ProGln: 1.249 ± 0.034	1.131ProArg: 1.131 ± 0.032	2.302ProSer: 2.302 ± 0.048	1.592ProThr: 1.592 ± 0.039	3.102ProVal: 3.102 ± 0.051	0.361ProTrp: 0.361 ± 0.016	1.391ProTyr: 1.391 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	2.404GlnAla: 2.404 ± 0.053	0.193GlnCys: 0.193 ± 0.014	1.697GlnAsp: 1.697 ± 0.048	3.061GlnGlu: 3.061 ± 0.066	1.581GlnPhe: 1.581 ± 0.04	2.353GlnGly: 2.353 ± 0.039	0.805GlnHis: 0.805 ± 0.027	2.028GlnIle: 2.028 ± 0.044	2.697GlnLys: 2.697 ± 0.056	3.442GlnLeu: 3.442 ± 0.055	1.092GlnMet: 1.092 ± 0.035	1.489GlnAsn: 1.489 ± 0.042	1.282GlnPro: 1.282 ± 0.037	1.659GlnGln: 1.659 ± 0.046	1.542GlnArg: 1.542 ± 0.04	2.132GlnSer: 2.132 ± 0.046	1.867GlnThr: 1.867 ± 0.044	2.032GlnVal: 2.032 ± 0.045	0.476GlnTrp: 0.476 ± 0.023	1.375GlnTyr: 1.375 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	2.293ArgAla: 2.293 ± 0.054	0.233ArgCys: 0.233 ± 0.015	2.131ArgAsp: 2.131 ± 0.049	3.485ArgGlu: 3.485 ± 0.063	1.912ArgPhe: 1.912 ± 0.045	2.543ArgGly: 2.543 ± 0.054	0.79ArgHis: 0.79 ± 0.029	3.025ArgIle: 3.025 ± 0.062	3.434ArgLys: 3.434 ± 0.062	3.932ArgLeu: 3.932 ± 0.063	1.423ArgMet: 1.423 ± 0.037	2.017ArgAsn: 2.017 ± 0.051	1.29ArgPro: 1.29 ± 0.036	1.46ArgGln: 1.46 ± 0.044	1.943ArgArg: 1.943 ± 0.052	2.367ArgSer: 2.367 ± 0.045	2.027ArgThr: 2.027 ± 0.044	2.54ArgVal: 2.54 ± 0.055	0.449ArgTrp: 0.449 ± 0.021	1.496ArgTyr: 1.496 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	4.194SerAla: 4.194 ± 0.062	0.388SerCys: 0.388 ± 0.018	2.958SerAsp: 2.958 ± 0.061	4.457SerGlu: 4.457 ± 0.077	3.472SerPhe: 3.472 ± 0.063	4.738SerGly: 4.738 ± 0.084	1.242SerHis: 1.242 ± 0.036	5.021SerIle: 5.021 ± 0.073	3.76SerLys: 3.76 ± 0.066	6.577SerLeu: 6.577 ± 0.093	1.839SerMet: 1.839 ± 0.041	2.44SerAsn: 2.44 ± 0.054	2.26SerPro: 2.26 ± 0.043	2.155SerGln: 2.155 ± 0.047	2.487SerArg: 2.487 ± 0.054	4.315SerSer: 4.315 ± 0.089	3.017SerThr: 3.017 ± 0.054	4.333SerVal: 4.333 ± 0.068	0.654SerTrp: 0.654 ± 0.028	2.335SerTyr: 2.335 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	4.077ThrAla: 4.077 ± 0.063	0.317ThrCys: 0.317 ± 0.017	2.699ThrAsp: 2.699 ± 0.054	3.761ThrGlu: 3.761 ± 0.068	2.526ThrPhe: 2.526 ± 0.049	4.271ThrGly: 4.271 ± 0.074	0.985ThrHis: 0.985 ± 0.03	4.159ThrIle: 4.159 ± 0.071	3.006ThrLys: 3.006 ± 0.046	5.267ThrLeu: 5.267 ± 0.073	1.304ThrMet: 1.304 ± 0.031	2.135ThrAsn: 2.135 ± 0.043	2.138ThrPro: 2.138 ± 0.045	1.264ThrGln: 1.264 ± 0.033	1.884ThrArg: 1.884 ± 0.04	3.201ThrSer: 3.201 ± 0.058	2.588ThrThr: 2.588 ± 0.056	4.013ThrVal: 4.013 ± 0.071	0.525ThrTrp: 0.525 ± 0.023	1.765ThrTyr: 1.765 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	4.232ValAla: 4.232 ± 0.069	0.57ValCys: 0.57 ± 0.025	3.651ValAsp: 3.651 ± 0.071	5.061ValGlu: 5.061 ± 0.091	3.285ValPhe: 3.285 ± 0.064	4.258ValGly: 4.258 ± 0.064	1.313ValHis: 1.313 ± 0.037	5.666ValIle: 5.666 ± 0.071	4.69ValLys: 4.69 ± 0.065	6.838ValLeu: 6.838 ± 0.087	1.888ValMet: 1.888 ± 0.047	3.113ValAsn: 3.113 ± 0.061	2.614ValPro: 2.614 ± 0.05	2.166ValGln: 2.166 ± 0.046	2.601ValArg: 2.601 ± 0.06	4.696ValSer: 4.696 ± 0.067	3.958ValThr: 3.958 ± 0.062	4.587ValVal: 4.587 ± 0.08	0.611ValTrp: 0.611 ± 0.026	2.27ValTyr: 2.27 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	0.687TrpAla: 0.687 ± 0.03	0.078TrpCys: 0.078 ± 0.009	0.506TrpAsp: 0.506 ± 0.022	0.749TrpGlu: 0.749 ± 0.03	0.509TrpPhe: 0.509 ± 0.024	0.715TrpGly: 0.715 ± 0.026	0.197TrpHis: 0.197 ± 0.014	0.771TrpIle: 0.771 ± 0.027	0.823TrpLys: 0.823 ± 0.028	1.151TrpLeu: 1.151 ± 0.034	0.378TrpMet: 0.378 ± 0.019	0.566TrpAsn: 0.566 ± 0.022	0.253TrpPro: 0.253 ± 0.018	0.338TrpGln: 0.338 ± 0.018	0.425TrpArg: 0.425 ± 0.021	0.663TrpSer: 0.663 ± 0.029	0.502TrpThr: 0.502 ± 0.022	0.668TrpVal: 0.668 ± 0.025	0.171TrpTrp: 0.171 ± 0.012	0.354TrpTyr: 0.354 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.85TyrAla: 1.85 ± 0.04	0.305TyrCys: 0.305 ± 0.016	1.801TyrAsp: 1.801 ± 0.048	2.642TyrGlu: 2.642 ± 0.054	1.963TyrPhe: 1.963 ± 0.052	2.419TyrGly: 2.419 ± 0.054	0.86TyrHis: 0.86 ± 0.03	2.503TyrIle: 2.503 ± 0.049	2.171TyrLys: 2.171 ± 0.046	3.503TyrLeu: 3.503 ± 0.057	0.881TyrMet: 0.881 ± 0.028	1.479TyrAsn: 1.479 ± 0.041	1.41TyrPro: 1.41 ± 0.036	1.506TyrGln: 1.506 ± 0.039	1.608TyrArg: 1.608 ± 0.04	2.293TyrSer: 2.293 ± 0.045	1.761TyrThr: 1.761 ± 0.039	1.98TyrVal: 1.98 ± 0.042	0.432TyrTrp: 0.432 ± 0.021	1.461TyrTyr: 1.461 ± 0.042	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4305 proteins (1053293 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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