Fermentimonas caenicola
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2391 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A098BZY2|A0A098BZY2_9BACT His_kinase domain-containing protein OS=Fermentimonas caenicola OX=1562970 GN=ING2E5B_0974 PE=4 SV=1
MM1 pKa = 7.61 LALTMAGIQSCSDD14 pKa = 3.45 EE15 pKa = 4.21 LHH17 pKa = 7.27 DD18 pKa = 4.59 VGDD21 pKa = 3.99 VQVSFTATLPTDD33 pKa = 2.91 TRR35 pKa = 11.84 TRR37 pKa = 11.84 SFGKK41 pKa = 10.29 AEE43 pKa = 3.95 QVNTLVVGIFKK54 pKa = 10.65 KK55 pKa = 10.93 GIADD59 pKa = 3.34 VHH61 pKa = 6.0 TNSGGSWNYY70 pKa = 10.48 YY71 pKa = 9.61 EE72 pKa = 5.53 IGRR75 pKa = 11.84 QSFPINGTSANVQLTLAQEE94 pKa = 3.94 QTYY97 pKa = 11.19 SFVFWAYY104 pKa = 10.75 DD105 pKa = 3.74 GNQSIYY111 pKa = 10.84 DD112 pKa = 3.8 IDD114 pKa = 4.6 DD115 pKa = 3.47 LTAIEE120 pKa = 5.32 MNTLPNPTTFAQAEE134 pKa = 3.96 AADD137 pKa = 3.98 AFFATMEE144 pKa = 5.58 DD145 pKa = 3.15 ITITGDD151 pKa = 2.99 CSYY154 pKa = 10.36 PVEE157 pKa = 4.31 LVRR160 pKa = 11.84 PLAQINVGTTGTPMQAVFKK179 pKa = 10.91 ANDD182 pKa = 3.53 VPDD185 pKa = 3.55 TFYY188 pKa = 10.87 PFTNTVSGAAEE199 pKa = 4.05 FTWNFSEE206 pKa = 4.52 TTTEE210 pKa = 4.0 TFSADD215 pKa = 2.98 GTEE218 pKa = 3.99 YY219 pKa = 11.02 NYY221 pKa = 11.18 LAMGYY226 pKa = 9.77 VFAPTTATNISAEE239 pKa = 4.14 LTLTDD244 pKa = 3.96 GNNSKK249 pKa = 8.36 TVEE252 pKa = 4.33 FPQVEE257 pKa = 4.04 IEE259 pKa = 3.96 ANQRR263 pKa = 11.84 SNIAGNFTPTEE274 pKa = 3.91
Molecular weight: 29.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.694
ProMoST 3.999
Dawson 3.859
Bjellqvist 4.05
Wikipedia 3.77
Rodwell 3.719
Grimsley 3.605
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.164
Thurlkill 3.732
EMBOSS 3.783
Sillero 3.999
Patrickios 1.1
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.906
Protein with the highest isoelectric point:
>tr|A0A098C2W4|A0A098C2W4_9BACT HTH domain protein OS=Fermentimonas caenicola OX=1562970 GN=ING2E5B_1484 PE=4 SV=1
MM1 pKa = 7.84 TIRR4 pKa = 11.84 NLTKK8 pKa = 10.74 EE9 pKa = 4.39 EE10 pKa = 3.87 ILDD13 pKa = 3.57 QIKK16 pKa = 10.72 YY17 pKa = 10.46 LEE19 pKa = 4.07 QNISNGSVSYY29 pKa = 8.93 RR30 pKa = 11.84 TNRR33 pKa = 11.84 LNRR36 pKa = 11.84 IRR38 pKa = 11.84 TLKK41 pKa = 10.65 ASLRR45 pKa = 11.84 MASS48 pKa = 3.22
Molecular weight: 5.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.794
IPC_protein 10.628
Toseland 10.687
ProMoST 10.394
Dawson 10.789
Bjellqvist 10.511
Wikipedia 11.008
Rodwell 11.008
Grimsley 10.847
Solomon 10.906
Lehninger 10.877
Nozaki 10.643
DTASelect 10.511
Thurlkill 10.687
EMBOSS 11.082
Sillero 10.716
Patrickios 10.833
IPC_peptide 10.921
IPC2_peptide 9.326
IPC2.peptide.svr19 8.555
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2391
0
2391
847865
30
2268
354.6
40.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.128 ± 0.043
0.811 ± 0.017
5.722 ± 0.031
6.96 ± 0.042
4.767 ± 0.035
6.658 ± 0.043
1.715 ± 0.019
8.135 ± 0.047
6.791 ± 0.045
9.007 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.532 ± 0.02
5.791 ± 0.043
3.687 ± 0.023
3.089 ± 0.026
4.394 ± 0.034
6.794 ± 0.039
5.339 ± 0.032
6.304 ± 0.037
1.165 ± 0.022
4.211 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here