Beihai tombus-like virus 8

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFR8|A0A1L3KFR8_9VIRU CCHC-type domain-containing protein OS=Beihai tombus-like virus 8 OX=1922729 PE=4 SV=1
MM1 pKa = 7.22SRR3 pKa = 11.84AVSNVPPSGTSKK15 pKa = 9.98PKK17 pKa = 10.17EE18 pKa = 3.92MRR20 pKa = 11.84CYY22 pKa = 9.26RR23 pKa = 11.84CDD25 pKa = 3.64GVGHH29 pKa = 6.24MARR32 pKa = 11.84DD33 pKa = 3.73CGPEE37 pKa = 3.32KK38 pKa = 10.2RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84GGRR44 pKa = 11.84GAGQARR50 pKa = 11.84RR51 pKa = 11.84AVRR54 pKa = 11.84VSDD57 pKa = 3.3EE58 pKa = 4.37SSRR61 pKa = 11.84HH62 pKa = 3.69VHH64 pKa = 6.46SCDD67 pKa = 2.95KK68 pKa = 11.07CLGLYY73 pKa = 9.86SHH75 pKa = 6.69LHH77 pKa = 6.0KK78 pKa = 10.99YY79 pKa = 7.16NHH81 pKa = 7.18KK82 pKa = 10.47DD83 pKa = 3.07HH84 pKa = 6.54EE85 pKa = 4.31QRR87 pKa = 11.84PYY89 pKa = 9.37QCPYY93 pKa = 9.73PNCEE97 pKa = 3.85WFVGAGSEE105 pKa = 4.31EE106 pKa = 4.13QSRR109 pKa = 11.84TVPLMLEE116 pKa = 4.24PPKK119 pKa = 10.98APAPPAQPTPEE130 pKa = 4.28PRR132 pKa = 11.84DD133 pKa = 3.67PLVGPPVDD141 pKa = 3.31AAPAEE146 pKa = 4.52EE147 pKa = 4.32EE148 pKa = 4.26TCPKK152 pKa = 10.38GFSSFSEE159 pKa = 4.23LRR161 pKa = 11.84DD162 pKa = 3.5HH163 pKa = 6.83LHH165 pKa = 7.53AYY167 pKa = 7.43LTKK170 pKa = 10.06QFAWSEE176 pKa = 4.06RR177 pKa = 11.84TVLNRR182 pKa = 11.84RR183 pKa = 11.84SMVQRR188 pKa = 11.84AEE190 pKa = 3.62TWLVSKK196 pKa = 10.62KK197 pKa = 10.62VEE199 pKa = 4.22SEE201 pKa = 4.09VLRR204 pKa = 11.84TQVIQAVIDD213 pKa = 3.72TVDD216 pKa = 3.0IKK218 pKa = 11.25NALEE222 pKa = 4.26SAVTTAAQSEE232 pKa = 4.75EE233 pKa = 3.73WRR235 pKa = 11.84NQHH238 pKa = 5.63IAANRR243 pKa = 11.84AAAGWLYY250 pKa = 10.74VPPKK254 pKa = 8.33PAGWTRR260 pKa = 11.84LEE262 pKa = 4.09KK263 pKa = 10.85VLVSIVLAFSCVTVVTLLGPGAMASAAGWLFSQVVTGTASLNMTILLANVAILLWRR319 pKa = 11.84GGQGGGPQPATPTEE333 pKa = 4.2VFRR336 pKa = 11.84AGTMGLGG343 pKa = 3.35

Molecular weight: 37.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFR8|A0A1L3KFR8_9VIRU CCHC-type domain-containing protein OS=Beihai tombus-like virus 8 OX=1922729 PE=4 SV=1
MM1 pKa = 7.46WNSIPGCYY9 pKa = 9.98NPFVHH14 pKa = 6.66SNCVRR19 pKa = 11.84NEE21 pKa = 3.76YY22 pKa = 10.61VAMRR26 pKa = 11.84DD27 pKa = 3.19RR28 pKa = 11.84VVGKK32 pKa = 10.51VPVPTEE38 pKa = 3.53RR39 pKa = 11.84GVRR42 pKa = 11.84LLRR45 pKa = 11.84RR46 pKa = 11.84YY47 pKa = 9.18ARR49 pKa = 11.84KK50 pKa = 9.12LVKK53 pKa = 10.34RR54 pKa = 11.84LGKK57 pKa = 8.83TDD59 pKa = 3.51EE60 pKa = 4.09LTYY63 pKa = 10.92DD64 pKa = 3.42QVISHH69 pKa = 4.64YY70 pKa = 10.26HH71 pKa = 4.5GRR73 pKa = 11.84KK74 pKa = 7.47KK75 pKa = 10.3RR76 pKa = 11.84RR77 pKa = 11.84YY78 pKa = 8.01EE79 pKa = 3.71AAKK82 pKa = 10.18EE83 pKa = 4.1SLGKK87 pKa = 10.75LPLTKK92 pKa = 10.1NDD94 pKa = 4.12SIIKK98 pKa = 10.38AFVKK102 pKa = 10.35SEE104 pKa = 4.04KK105 pKa = 9.83FDD107 pKa = 3.81PAAKK111 pKa = 10.1VNPAPRR117 pKa = 11.84MIQARR122 pKa = 11.84NARR125 pKa = 11.84YY126 pKa = 9.65NLAIARR132 pKa = 11.84WLRR135 pKa = 11.84PIEE138 pKa = 3.83HH139 pKa = 7.31AIYY142 pKa = 10.11RR143 pKa = 11.84LKK145 pKa = 10.94SRR147 pKa = 11.84LTGLPIVGKK156 pKa = 10.28GRR158 pKa = 11.84SLTEE162 pKa = 3.72RR163 pKa = 11.84AEE165 pKa = 4.06LLAKK169 pKa = 9.97KK170 pKa = 10.57FSYY173 pKa = 10.1FKK175 pKa = 10.91HH176 pKa = 5.28PVVYY180 pKa = 10.7SLDD183 pKa = 3.37ASRR186 pKa = 11.84WDD188 pKa = 3.29QHH190 pKa = 7.95CDD192 pKa = 2.87IKK194 pKa = 11.15LLEE197 pKa = 4.16VEE199 pKa = 3.7HH200 pKa = 6.79WIYY203 pKa = 10.37KK204 pKa = 7.89TMNGSSEE211 pKa = 4.18FASLLQQQLHH221 pKa = 5.33NRR223 pKa = 11.84CFTEE227 pKa = 4.02HH228 pKa = 6.64GIAYY232 pKa = 6.76KK233 pKa = 8.55TTGKK237 pKa = 10.48RR238 pKa = 11.84MSGDD242 pKa = 3.14VNTALGNCLLAVLLAYY258 pKa = 10.13VVLVDD263 pKa = 3.4MLGLRR268 pKa = 11.84EE269 pKa = 4.25TEE271 pKa = 5.11FEE273 pKa = 4.52LLDD276 pKa = 5.14DD277 pKa = 5.25GDD279 pKa = 4.37DD280 pKa = 3.64LLVIVEE286 pKa = 4.22QADD289 pKa = 3.47EE290 pKa = 4.28HH291 pKa = 7.05RR292 pKa = 11.84LEE294 pKa = 4.96GIKK297 pKa = 9.75EE298 pKa = 3.95AYY300 pKa = 10.38LEE302 pKa = 4.23VGHH305 pKa = 6.54EE306 pKa = 3.94IKK308 pKa = 10.85LEE310 pKa = 3.8NRR312 pKa = 11.84ATVMEE317 pKa = 4.26DD318 pKa = 4.03VEE320 pKa = 4.72WCQHH324 pKa = 5.6RR325 pKa = 11.84PVLTPDD331 pKa = 2.82GWRR334 pKa = 11.84FVPNWRR340 pKa = 11.84KK341 pKa = 9.54VLSSTTTDD349 pKa = 3.08TKK351 pKa = 11.11HH352 pKa = 5.51WMHH355 pKa = 6.67EE356 pKa = 4.07NLRR359 pKa = 11.84PALAHH364 pKa = 5.98TMGKK368 pKa = 9.67CLLSMYY374 pKa = 10.59AGLPVLQEE382 pKa = 3.82YY383 pKa = 10.48CQFLIRR389 pKa = 11.84QGSKK393 pKa = 10.26DD394 pKa = 3.64AKK396 pKa = 10.0CLKK399 pKa = 10.62DD400 pKa = 4.55HH401 pKa = 7.06FLQDD405 pKa = 2.89RR406 pKa = 11.84AYY408 pKa = 8.85TNGGPTQTTKK418 pKa = 10.6TIDD421 pKa = 3.05PMTRR425 pKa = 11.84LSFQRR430 pKa = 11.84AYY432 pKa = 10.74GLTVQDD438 pKa = 3.91QLDD441 pKa = 4.05LEE443 pKa = 4.46TRR445 pKa = 11.84IRR447 pKa = 11.84GLTLPTTTLRR457 pKa = 11.84VTASEE462 pKa = 4.37VTPGWNWEE470 pKa = 4.22YY471 pKa = 11.54YY472 pKa = 9.5PGTEE476 pKa = 4.58AGLGG480 pKa = 3.55

Molecular weight: 55.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

823

343

480

411.5

46.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.262 ± 1.284

2.187 ± 0.251

4.131 ± 0.699

6.44 ± 0.015

2.309 ± 0.154

6.926 ± 0.544

3.159 ± 0.14

3.038 ± 0.573

5.468 ± 0.797

9.964 ± 1.371

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.309 ± 0.014

3.159 ± 0.308

5.954 ± 1.271

3.645 ± 0.251

7.776 ± 0.113

5.589 ± 0.977

6.561 ± 0.42

7.655 ± 0.628

2.187 ± 0.084

3.281 ± 0.713
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski