Primula malacoides virus China/Mar2007
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A8W7G2|A8W7G2_9VIRU Putative capsid protein OS=Primula malacoides virus China/Mar2007 OX=479713 PE=4 SV=1
MM1 pKa = 7.81 PFNAVRR7 pKa = 11.84 NYY9 pKa = 10.0 LAEE12 pKa = 3.97 RR13 pKa = 11.84 LVRR16 pKa = 11.84 VRR18 pKa = 11.84 TEE20 pKa = 3.93 LKK22 pKa = 10.74 NFTSSNRR29 pKa = 11.84 EE30 pKa = 3.18 PDD32 pKa = 3.15 ATLEE36 pKa = 4.05 LSQDD40 pKa = 3.3 PDD42 pKa = 2.96 LRR44 pKa = 11.84 RR45 pKa = 11.84 YY46 pKa = 9.82 YY47 pKa = 11.24 DD48 pKa = 3.19 NTRR51 pKa = 11.84 YY52 pKa = 10.27 NSSNDD57 pKa = 2.93 AKK59 pKa = 10.98 YY60 pKa = 8.34 RR61 pKa = 11.84 TLNKK65 pKa = 10.05 EE66 pKa = 3.51 YY67 pKa = 9.43 STLVEE72 pKa = 5.25 AYY74 pKa = 8.27 RR75 pKa = 11.84 TDD77 pKa = 3.38 NQQKK81 pKa = 7.73 HH82 pKa = 4.96 QPYY85 pKa = 9.34 EE86 pKa = 3.75 LHH88 pKa = 6.15 QPIPADD94 pKa = 3.47 AAPIIDD100 pKa = 4.23 KK101 pKa = 10.31 RR102 pKa = 11.84 QPAPGLKK109 pKa = 9.6 LVPLMYY115 pKa = 10.21 HH116 pKa = 6.08 YY117 pKa = 11.23 GHH119 pKa = 7.13 VIHH122 pKa = 7.49 DD123 pKa = 4.88 PDD125 pKa = 5.4 PDD127 pKa = 3.61 QPDD130 pKa = 3.36 QTKK133 pKa = 10.21 VYY135 pKa = 9.33 PLDD138 pKa = 3.46 SRR140 pKa = 11.84 IYY142 pKa = 8.53 NLIMSTYY149 pKa = 8.84 PSYY152 pKa = 11.38 LSVLHH157 pKa = 6.91 DD158 pKa = 3.61 YY159 pKa = 10.18 CRR161 pKa = 11.84 PIGTVEE167 pKa = 3.7 ATFNDD172 pKa = 4.12 FNTEE176 pKa = 4.13 QIPSAPIDD184 pKa = 3.79 EE185 pKa = 4.82 DD186 pKa = 3.66 RR187 pKa = 11.84 KK188 pKa = 9.64 QQILKK193 pKa = 10.22 HH194 pKa = 5.58 LFKK197 pKa = 10.95 FLDD200 pKa = 3.63 VKK202 pKa = 10.69 PYY204 pKa = 10.98 LPIHH208 pKa = 6.15 FVDD211 pKa = 3.64 TQYY214 pKa = 11.57 CKK216 pKa = 10.19 TPLVTGTGYY225 pKa = 10.81 HH226 pKa = 5.59 NRR228 pKa = 11.84 YY229 pKa = 9.38 SFKK232 pKa = 10.55 QRR234 pKa = 11.84 AHH236 pKa = 6.38 AKK238 pKa = 8.37 YY239 pKa = 10.09 SHH241 pKa = 6.76 PEE243 pKa = 3.6 EE244 pKa = 4.37 YY245 pKa = 10.6 ALKK248 pKa = 8.8 STSKK252 pKa = 10.77 GYY254 pKa = 10.32 FYY256 pKa = 11.17 NATYY260 pKa = 10.79 EE261 pKa = 3.99 NARR264 pKa = 11.84 TLVHH268 pKa = 7.17 LIKK271 pKa = 10.08 TYY273 pKa = 10.8 GLPFNMQFACPKK285 pKa = 10.43 SDD287 pKa = 4.26 LTDD290 pKa = 3.52 EE291 pKa = 4.48 QINLYY296 pKa = 9.73 ISKK299 pKa = 10.67 ANQFFNDD306 pKa = 3.6 YY307 pKa = 7.51 PTLLFTRR314 pKa = 11.84 NHH316 pKa = 5.35 ISKK319 pKa = 10.63 RR320 pKa = 11.84 SGTLKK325 pKa = 10.07 VRR327 pKa = 11.84 PVYY330 pKa = 10.59 AVDD333 pKa = 4.8 DD334 pKa = 3.66 IFIIIEE340 pKa = 4.07 LMLTFPLTIQARR352 pKa = 11.84 KK353 pKa = 8.74 PSCCIMYY360 pKa = 10.42 GLEE363 pKa = 4.17 TIRR366 pKa = 11.84 GSNHH370 pKa = 6.08 YY371 pKa = 9.39 IDD373 pKa = 4.88 RR374 pKa = 11.84 LARR377 pKa = 11.84 PYY379 pKa = 10.35 STFFSLDD386 pKa = 2.74 WSSYY390 pKa = 6.99 DD391 pKa = 3.17 QRR393 pKa = 11.84 LPRR396 pKa = 11.84 VITDD400 pKa = 3.3 IYY402 pKa = 9.28 YY403 pKa = 9.62 TDD405 pKa = 4.32 FLRR408 pKa = 11.84 SLIVINHH415 pKa = 7.09 GYY417 pKa = 9.18 QPTYY421 pKa = 10.39 EE422 pKa = 4.22 YY423 pKa = 8.37 PTYY426 pKa = 10.44 PDD428 pKa = 3.72 LDD430 pKa = 3.56 EE431 pKa = 5.2 HH432 pKa = 6.91 KK433 pKa = 10.43 LYY435 pKa = 11.32 SRR437 pKa = 11.84 MDD439 pKa = 3.37 NLLYY443 pKa = 10.37 FLHH446 pKa = 6.14 TWYY449 pKa = 11.35 NNMTFLLPDD458 pKa = 4.16 GYY460 pKa = 11.15 AYY462 pKa = 10.68 RR463 pKa = 11.84 RR464 pKa = 11.84 THH466 pKa = 6.4 CGVPSGLYY474 pKa = 7.56 NTQYY478 pKa = 11.31 LDD480 pKa = 3.35 SFGNLFLIIDD490 pKa = 3.86 AMIEE494 pKa = 4.0 FEE496 pKa = 4.25 FTDD499 pKa = 3.95 DD500 pKa = 4.72 EE501 pKa = 4.35 ISKK504 pKa = 9.98 FQLLILGGDD513 pKa = 3.62 NTGMTNLAIDD523 pKa = 5.24 RR524 pKa = 11.84 IDD526 pKa = 3.36 KK527 pKa = 10.75 FITFLEE533 pKa = 5.07 TYY535 pKa = 10.78 ALARR539 pKa = 11.84 YY540 pKa = 10.2 NMVLPKK546 pKa = 9.26 TKK548 pKa = 10.53 CILTSLRR555 pKa = 11.84 SKK557 pKa = 10.46 IVTLGYY563 pKa = 9.02 EE564 pKa = 4.3 CNYY567 pKa = 10.2 GSPKK571 pKa = 9.94 RR572 pKa = 11.84 DD573 pKa = 2.66 IHH575 pKa = 7.87 KK576 pKa = 10.53 LVAQLCFPEE585 pKa = 5.29 NGLKK589 pKa = 10.23 AHH591 pKa = 5.92 TMSARR596 pKa = 11.84 ATGIAYY602 pKa = 9.9 ASAGQDD608 pKa = 2.64 IMFHH612 pKa = 5.81 SFCQDD617 pKa = 2.32 VYY619 pKa = 11.4 NIFRR623 pKa = 11.84 SDD625 pKa = 3.49 YY626 pKa = 10.75 KK627 pKa = 10.75 PDD629 pKa = 3.1 VRR631 pKa = 11.84 ANLYY635 pKa = 7.78 FQRR638 pKa = 11.84 QFLNDD643 pKa = 3.81 LEE645 pKa = 4.72 EE646 pKa = 4.49 GVPDD650 pKa = 4.51 LATPTVPPFPSLYY663 pKa = 9.97 EE664 pKa = 3.71 IRR666 pKa = 11.84 KK667 pKa = 9.74 LYY669 pKa = 10.74 SKK671 pKa = 10.8 YY672 pKa = 10.09 QGPLSFTPKK681 pKa = 9.09 WNSAHH686 pKa = 7.3 FINEE690 pKa = 3.97 PDD692 pKa = 3.82 SVPISAKK699 pKa = 7.56 TMRR702 pKa = 11.84 QFEE705 pKa = 4.57 EE706 pKa = 4.47 EE707 pKa = 3.96 FSIPLMTAATFEE719 pKa = 4.64 TVVPP723 pKa = 4.51
Molecular weight: 84.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.094
IPC2_protein 6.186
IPC_protein 6.287
Toseland 6.62
ProMoST 6.722
Dawson 6.649
Bjellqvist 6.634
Wikipedia 6.664
Rodwell 6.649
Grimsley 6.766
Solomon 6.649
Lehninger 6.649
Nozaki 6.927
DTASelect 7.102
Thurlkill 7.117
EMBOSS 7.102
Sillero 7.044
Patrickios 3.401
IPC_peptide 6.664
IPC2_peptide 6.868
IPC2.peptide.svr19 6.849
Protein with the highest isoelectric point:
>tr|A8W7G2|A8W7G2_9VIRU Putative capsid protein OS=Primula malacoides virus China/Mar2007 OX=479713 PE=4 SV=1
MM1 pKa = 7.81 SDD3 pKa = 2.97 TVLQAKK9 pKa = 8.92 TNFLKK14 pKa = 10.97 NKK16 pKa = 10.01 SPVLSVQEE24 pKa = 4.15 SKK26 pKa = 10.7 RR27 pKa = 11.84 PKK29 pKa = 9.63 ISRR32 pKa = 11.84 DD33 pKa = 3.41 LMPLFQSNTAARR45 pKa = 11.84 SDD47 pKa = 4.47 SSNQWTIDD55 pKa = 3.26 VFPNMVPITMYY66 pKa = 11.06 IMLTSIAHH74 pKa = 6.28 ANDD77 pKa = 2.82 VDD79 pKa = 3.51 HH80 pKa = 6.89 RR81 pKa = 11.84 TTSKK85 pKa = 10.83 VSVATICMYY94 pKa = 10.53 HH95 pKa = 7.74 LSIVYY100 pKa = 9.77 GYY102 pKa = 10.95 FLLNDD107 pKa = 4.23 MITRR111 pKa = 11.84 ASASAHH117 pKa = 4.79 ARR119 pKa = 11.84 PWIEE123 pKa = 3.89 TSWKK127 pKa = 10.46 RR128 pKa = 11.84 EE129 pKa = 3.71 FADD132 pKa = 4.66 FLLTLPVPEE141 pKa = 4.83 FLTPFLSQFQHH152 pKa = 5.65 FTTDD156 pKa = 2.65 RR157 pKa = 11.84 TKK159 pKa = 11.2 NVFLTPSAAGFDD171 pKa = 3.75 HH172 pKa = 7.12 NIFFGRR178 pKa = 11.84 VFPLNMFSAIHH189 pKa = 7.12 DD190 pKa = 4.03 CTATLPGNTPRR201 pKa = 11.84 IQVLQDD207 pKa = 3.45 LYY209 pKa = 11.79 SRR211 pKa = 11.84 VLYY214 pKa = 9.92 TITAPGFTCLIPDD227 pKa = 5.29 LIGVTIDD234 pKa = 3.09 QTTPTTANYY243 pKa = 7.91 MNSKK247 pKa = 10.1 LYY249 pKa = 10.06 QVYY252 pKa = 7.91 TAVFNPVLFRR262 pKa = 11.84 DD263 pKa = 4.41 FQHH266 pKa = 6.84 RR267 pKa = 11.84 SSLAALSFQSPVFANNHH284 pKa = 4.01 VNAYY288 pKa = 10.02 DD289 pKa = 3.97 LLFSASAANLRR300 pKa = 11.84 EE301 pKa = 4.29 LKK303 pKa = 10.65 VVLQVVSTVLDD314 pKa = 4.01 GKK316 pKa = 9.33 VTTSGTLGDD325 pKa = 4.83 FLTKK329 pKa = 10.28 PSGALAIKK337 pKa = 10.15 HH338 pKa = 6.07 GYY340 pKa = 7.32 STYY343 pKa = 11.44 ALPTWSYY350 pKa = 8.77 NTNATKK356 pKa = 10.53 SAVYY360 pKa = 10.16 SAITTLTLTSEE371 pKa = 4.19 RR372 pKa = 11.84 DD373 pKa = 3.3 RR374 pKa = 11.84 AQDD377 pKa = 2.67 ICFLQRR383 pKa = 11.84 PAAAFNATTEE393 pKa = 4.39 VTDD396 pKa = 3.3 VTYY399 pKa = 10.79 AATATPNQAVQLPTGHH415 pKa = 6.38 QLTRR419 pKa = 11.84 HH420 pKa = 5.74 FPFSLLQVPNGAEE433 pKa = 3.69 PFPRR437 pKa = 11.84 HH438 pKa = 6.69 DD439 pKa = 4.47 NDD441 pKa = 5.27 DD442 pKa = 3.81 LVQFSDD448 pKa = 4.81 FVHH451 pKa = 6.43 TAPSVLVLDD460 pKa = 4.18 TDD462 pKa = 4.33 GEE464 pKa = 4.52 LTISAHH470 pKa = 6.48 LPLLAGKK477 pKa = 9.71 IIEE480 pKa = 4.27 SFEE483 pKa = 4.19 IDD485 pKa = 3.09 GSTIEE490 pKa = 4.59 MPNVDD495 pKa = 4.9 KK496 pKa = 11.33 SLGLQNCLFADD507 pKa = 3.64 SAVSYY512 pKa = 10.6 RR513 pKa = 11.84 LVRR516 pKa = 11.84 PGSAFHH522 pKa = 6.33 PQPAGSINPPLNRR535 pKa = 11.84 VRR537 pKa = 11.84 PNPRR541 pKa = 11.84 PRR543 pKa = 11.84 LPASSLLYY551 pKa = 10.72 DD552 pKa = 3.67 RR553 pKa = 11.84 TEE555 pKa = 3.52 IMLPNMNNRR564 pKa = 11.84 IVEE567 pKa = 3.99 ARR569 pKa = 11.84 APDD572 pKa = 4.02 TLPGMTRR579 pKa = 11.84 RR580 pKa = 11.84 AAVNFLRR587 pKa = 11.84 YY588 pKa = 8.96 AQSFFGFHH596 pKa = 5.85 TVDD599 pKa = 3.04 SSSNALSLDD608 pKa = 4.26 TIPGMTNGLLHH619 pKa = 6.79 LWSPYY624 pKa = 10.56 SYY626 pKa = 10.95 TPFEE630 pKa = 4.16 NDD632 pKa = 3.39 YY633 pKa = 11.28 FPDD636 pKa = 4.68 PDD638 pKa = 3.68 LAEE641 pKa = 4.2 SRR643 pKa = 11.84 HH644 pKa = 5.29 YY645 pKa = 10.56 FLSNLRR651 pKa = 11.84 TIFGTDD657 pKa = 3.34 YY658 pKa = 11.38 NLVQAKK664 pKa = 9.69 HH665 pKa = 6.38 PYY667 pKa = 8.61 EE668 pKa = 4.31 ALPVVV673 pKa = 3.92
Molecular weight: 74.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.121
IPC2_protein 6.033
IPC_protein 6.148
Toseland 6.62
ProMoST 6.561
Dawson 6.491
Bjellqvist 6.465
Wikipedia 6.517
Rodwell 6.478
Grimsley 6.81
Solomon 6.491
Lehninger 6.491
Nozaki 6.766
DTASelect 6.971
Thurlkill 6.985
EMBOSS 6.956
Sillero 6.883
Patrickios 4.012
IPC_peptide 6.504
IPC2_peptide 6.825
IPC2.peptide.svr19 6.773
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1396
673
723
698.0
79.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.948 ± 1.104
1.074 ± 0.219
6.16 ± 0.34
3.51 ± 0.65
5.874 ± 0.242
3.51 ± 0.037
3.009 ± 0.024
5.301 ± 0.656
3.797 ± 0.84
10.172 ± 0.25
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.077 ± 0.002
5.372 ± 0.181
7.092 ± 0.125
3.725 ± 0.105
5.229 ± 0.215
7.235 ± 0.816
8.309 ± 0.695
5.301 ± 0.916
0.573 ± 0.112
5.731 ± 1.431
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here