Endogone sp. FLAS-F59071
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9431 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A433PLB9|A0A433PLB9_9FUNG Uncharacterized protein OS=Endogone sp. FLAS-F59071 OX=2340872 GN=BC937DRAFT_89004 PE=4 SV=1
MM1 pKa = 7.33 YY2 pKa = 10.28 CPGMYY7 pKa = 9.97 YY8 pKa = 10.33 KK9 pKa = 10.68 SSPLHH14 pKa = 6.78 HH15 pKa = 6.77 NLQPDD20 pKa = 3.6 VGSASPSGINYY31 pKa = 10.08 SAITTDD37 pKa = 3.02 LHH39 pKa = 6.52 FPAEE43 pKa = 4.33 GLVSSIVPGGTVPEE57 pKa = 4.5 GTVPEE62 pKa = 4.24 DD63 pKa = 3.63 TVPEE67 pKa = 4.32 GTVPEE72 pKa = 4.3 DD73 pKa = 3.6 TVPEE77 pKa = 4.13 DD78 pKa = 3.81 TVPEE82 pKa = 4.19 DD83 pKa = 3.64 TVPEE87 pKa = 4.16 YY88 pKa = 9.92 TVPEE92 pKa = 4.16 DD93 pKa = 3.58 TVPEE97 pKa = 4.15 VLEE100 pKa = 4.29 GTVPEE105 pKa = 4.41 VPEE108 pKa = 4.07 DD109 pKa = 3.87 TVPEE113 pKa = 4.1 DD114 pKa = 3.92 TVPVDD119 pKa = 4.23 ALAADD124 pKa = 3.83 TALACSTAPADD135 pKa = 3.85 TDD137 pKa = 3.9 PAADD141 pKa = 3.8 SAPADD146 pKa = 3.79 TAPVGDD152 pKa = 4.11 TAWANPATGMPRR164 pKa = 11.84 KK165 pKa = 10.16 VKK167 pKa = 10.04 VRR169 pKa = 11.84 CSQSARR175 pKa = 11.84 GGCWRR180 pKa = 11.84 QAA182 pKa = 3.07
Molecular weight: 18.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.821
IPC_protein 3.795
Toseland 3.592
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.63
Grimsley 3.503
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.101
Thurlkill 3.643
EMBOSS 3.719
Sillero 3.923
Patrickios 1.036
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.81
Protein with the highest isoelectric point:
>tr|A0A433PTP4|A0A433PTP4_9FUNG Uncharacterized protein OS=Endogone sp. FLAS-F59071 OX=2340872 GN=BC937DRAFT_94075 PE=4 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.41 LVRR5 pKa = 11.84 LTKK8 pKa = 10.66 LLVRR12 pKa = 11.84 STKK15 pKa = 10.75 LLVKK19 pKa = 10.81 SMMKK23 pKa = 10.49 LVRR26 pKa = 11.84 LMTKK30 pKa = 10.53 LLVKK34 pKa = 10.86 SMTKK38 pKa = 10.49 LVRR41 pKa = 11.84 STTLVRR47 pKa = 11.84 LTKK50 pKa = 10.56 LLLVRR55 pKa = 11.84 STKK58 pKa = 10.74 LLVRR62 pKa = 11.84 STTTKK67 pKa = 10.43 LVRR70 pKa = 11.84 SMKK73 pKa = 10.14 LVRR76 pKa = 11.84 STKK79 pKa = 10.31 LLVRR83 pKa = 11.84 STTKK87 pKa = 10.79 LLVRR91 pKa = 11.84 STTKK95 pKa = 10.52 LVRR98 pKa = 11.84 STKK101 pKa = 10.04 LVRR104 pKa = 11.84 STKK107 pKa = 10.42 LLVRR111 pKa = 11.84 STTKK115 pKa = 10.75 LVTTKK120 pKa = 10.54 LVRR123 pKa = 11.84 STTTKK128 pKa = 10.51 LVRR131 pKa = 11.84 STKK134 pKa = 10.04 LVRR137 pKa = 11.84 STKK140 pKa = 10.44 LLVRR144 pKa = 11.84 SMTKK148 pKa = 10.48 LLVRR152 pKa = 11.84 SMATKK157 pKa = 10.64 LVRR160 pKa = 11.84 STKK163 pKa = 10.29 LLVRR167 pKa = 11.84 STMTKK172 pKa = 10.28 LVRR175 pKa = 11.84 STKK178 pKa = 10.42 LLVRR182 pKa = 11.84 STTKK186 pKa = 10.79 LLVRR190 pKa = 11.84 STTTKK195 pKa = 10.51 LVRR198 pKa = 11.84 STKK201 pKa = 10.01 LVRR204 pKa = 11.84 SMKK207 pKa = 10.09 LVRR210 pKa = 11.84 SMTKK214 pKa = 10.3 LLVRR218 pKa = 11.84 STATKK223 pKa = 10.38 LVRR226 pKa = 11.84 STKK229 pKa = 10.04 LVRR232 pKa = 11.84 STKK235 pKa = 10.42 LLVRR239 pKa = 11.84 STTKK243 pKa = 10.83 LVTTKK248 pKa = 10.85 LLVRR252 pKa = 11.84 STTTKK257 pKa = 10.81 LMRR260 pKa = 11.84 STKK263 pKa = 9.97 LVRR266 pKa = 11.84 STKK269 pKa = 10.58 LLVRR273 pKa = 11.84 STKK276 pKa = 10.55 LVTTKK281 pKa = 10.83 LVTTKK286 pKa = 10.63 LVRR289 pKa = 11.84 STKK292 pKa = 10.46 LLLRR296 pKa = 11.84 STTTKK301 pKa = 10.55 LVRR304 pKa = 11.84 STKK307 pKa = 10.07 LVRR310 pKa = 11.84 LTKK313 pKa = 10.56 LLVRR317 pKa = 11.84 SRR319 pKa = 11.84 LEE321 pKa = 4.23 FTCHH325 pKa = 5.89
Molecular weight: 37.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.514
IPC2_protein 11.389
IPC_protein 12.939
Toseland 13.115
ProMoST 13.598
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.583
Rodwell 12.954
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.661
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.232
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9193
238
9431
3895637
8
5502
413.1
46.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.835 ± 0.024
1.326 ± 0.011
5.576 ± 0.021
6.456 ± 0.032
3.894 ± 0.017
6.117 ± 0.023
2.459 ± 0.011
5.257 ± 0.018
5.087 ± 0.023
9.139 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.213 ± 0.011
4.017 ± 0.016
5.785 ± 0.029
3.945 ± 0.023
6.01 ± 0.021
8.067 ± 0.03
6.211 ± 0.021
6.498 ± 0.02
1.235 ± 0.008
2.873 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here