Oceanospirillum multiglobuliferum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Oceanospirillum

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3411 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T4MWF0|A0A1T4MWF0_9GAMM Uncharacterized protein OS=Oceanospirillum multiglobuliferum OX=64969 GN=BTE48_00075 PE=4 SV=1
MM1 pKa = 6.46QTLVCSCPEE10 pKa = 4.48LKK12 pKa = 10.88FNDD15 pKa = 3.26QGTAQEE21 pKa = 5.1LIVSAIKK28 pKa = 10.57QNLPRR33 pKa = 11.84LKK35 pKa = 10.54GVTASGEE42 pKa = 4.36VIDD45 pKa = 5.58LPEE48 pKa = 4.77LSEE51 pKa = 6.05ILLEE55 pKa = 4.11WDD57 pKa = 3.28EE58 pKa = 4.56GLPATLLLSPEE69 pKa = 4.45HH70 pKa = 6.99LDD72 pKa = 4.22GPALTLEE79 pKa = 4.32VGSAEE84 pKa = 5.03DD85 pKa = 3.79EE86 pKa = 4.32DD87 pKa = 5.26DD88 pKa = 4.57ALTEE92 pKa = 3.78YY93 pKa = 10.83GIIVLRR99 pKa = 11.84PGASNMVDD107 pKa = 3.06ISIEE111 pKa = 4.03THH113 pKa = 4.66VVEE116 pKa = 6.04DD117 pKa = 4.29EE118 pKa = 4.62NCGEE122 pKa = 4.9DD123 pKa = 4.89CNCHH127 pKa = 6.55DD128 pKa = 4.53

Molecular weight: 13.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T4P259|A0A1T4P259_9GAMM Uncharacterized protein OS=Oceanospirillum multiglobuliferum OX=64969 GN=BTE48_10680 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.43NGRR28 pKa = 11.84KK29 pKa = 9.65LIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.99

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3411

0

3411

1060053

19

3908

310.8

34.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.29 ± 0.046

0.983 ± 0.016

5.255 ± 0.046

6.294 ± 0.039

3.817 ± 0.026

6.712 ± 0.038

2.278 ± 0.021

6.069 ± 0.032

4.783 ± 0.036

11.107 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.022

3.889 ± 0.03

4.085 ± 0.034

5.497 ± 0.049

4.989 ± 0.034

6.468 ± 0.042

5.164 ± 0.033

6.752 ± 0.037

1.242 ± 0.018

2.813 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski