Bemisia-associated genomovirus AdO
Average proteome isoelectric point is 7.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z2X292|A0A1Z2X292_9VIRU Putative coat protein OS=Bemisia-associated genomovirus AdO OX=1986478 GN=CP PE=4 SV=1
MM1 pKa = 7.75 PSFVHH6 pKa = 6.39 NYY8 pKa = 9.2 RR9 pKa = 11.84 YY10 pKa = 10.74 ALVTYY15 pKa = 9.62 SQYY18 pKa = 11.76 GDD20 pKa = 3.79 LDD22 pKa = 3.28 PWRR25 pKa = 11.84 VMEE28 pKa = 4.64 RR29 pKa = 11.84 FSSLGAEE36 pKa = 4.14 CIVARR41 pKa = 11.84 EE42 pKa = 4.01 HH43 pKa = 7.16 HH44 pKa = 6.88 EE45 pKa = 4.44 DD46 pKa = 3.61 GGLHH50 pKa = 5.69 LHH52 pKa = 6.24 VFADD56 pKa = 4.7 FGRR59 pKa = 11.84 KK60 pKa = 8.16 FRR62 pKa = 11.84 SRR64 pKa = 11.84 RR65 pKa = 11.84 TDD67 pKa = 2.87 ILDD70 pKa = 3.06 VDD72 pKa = 3.87 GRR74 pKa = 11.84 HH75 pKa = 6.37 ANVEE79 pKa = 4.17 PSAGTPEE86 pKa = 3.94 KK87 pKa = 10.83 GYY89 pKa = 10.94 DD90 pKa = 3.47 YY91 pKa = 10.69 AIKK94 pKa = 10.83 DD95 pKa = 3.47 GDD97 pKa = 4.3 VVCGGLARR105 pKa = 11.84 PEE107 pKa = 3.94 PSAVGDD113 pKa = 3.9 GPTAAKK119 pKa = 8.46 WARR122 pKa = 11.84 ITSAEE127 pKa = 4.15 TRR129 pKa = 11.84 SEE131 pKa = 3.97 FWKK134 pKa = 10.52 LCHH137 pKa = 6.51 EE138 pKa = 5.45 LDD140 pKa = 4.41 PKK142 pKa = 10.92 AAACNFSALSKK153 pKa = 8.92 YY154 pKa = 10.36 CDD156 pKa = 2.81 WRR158 pKa = 11.84 FAEE161 pKa = 5.18 DD162 pKa = 3.76 PPEE165 pKa = 4.16 YY166 pKa = 10.48 EE167 pKa = 3.29 HH168 pKa = 7.62 DD169 pKa = 3.67 GRR171 pKa = 11.84 IKK173 pKa = 10.38 FVPGDD178 pKa = 3.4 GDD180 pKa = 4.83 GRR182 pKa = 11.84 DD183 pKa = 3.51 DD184 pKa = 3.48 WVSQSGIRR192 pKa = 11.84 LDD194 pKa = 3.93 EE195 pKa = 4.75 PFLGMWVFAPNRR207 pKa = 11.84 VKK209 pKa = 10.93 SLVLYY214 pKa = 10.75 GGTRR218 pKa = 11.84 TGKK221 pKa = 5.93 TTWARR226 pKa = 11.84 SLGSHH231 pKa = 6.56 VYY233 pKa = 10.64 CIGLVSGTEE242 pKa = 4.09 CARR245 pKa = 11.84 GLDD248 pKa = 3.23 AKK250 pKa = 10.93 YY251 pKa = 10.81 AVFDD255 pKa = 5.03 DD256 pKa = 3.77 IRR258 pKa = 11.84 GGLAFFHH265 pKa = 7.2 GYY267 pKa = 9.97 KK268 pKa = 9.68 EE269 pKa = 3.96 WLGAQPHH276 pKa = 5.31 VSIKK280 pKa = 9.68 QLYY283 pKa = 8.38 RR284 pKa = 11.84 EE285 pKa = 4.38 PYY287 pKa = 7.41 YY288 pKa = 10.35 MRR290 pKa = 11.84 WGKK293 pKa = 7.47 PTIWVCNTDD302 pKa = 3.55 PRR304 pKa = 11.84 LDD306 pKa = 4.29 AYY308 pKa = 9.24 PQNARR313 pKa = 11.84 PDD315 pKa = 4.03 FEE317 pKa = 4.46 WMEE320 pKa = 4.29 GNCTFIEE327 pKa = 4.49 VTDD330 pKa = 3.95 SLIEE334 pKa = 4.57 SISHH338 pKa = 6.9 ANTDD342 pKa = 3.19
Molecular weight: 38.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.411
IPC2_protein 5.41
IPC_protein 5.423
Toseland 5.715
ProMoST 5.626
Dawson 5.614
Bjellqvist 5.664
Wikipedia 5.588
Rodwell 5.575
Grimsley 5.83
Solomon 5.614
Lehninger 5.601
Nozaki 5.842
DTASelect 6.033
Thurlkill 5.957
EMBOSS 5.931
Sillero 5.931
Patrickios 3.834
IPC_peptide 5.626
IPC2_peptide 5.944
IPC2.peptide.svr19 5.883
Protein with the highest isoelectric point:
>tr|A0A1Z2X292|A0A1Z2X292_9VIRU Putative coat protein OS=Bemisia-associated genomovirus AdO OX=1986478 GN=CP PE=4 SV=1
MM1 pKa = 7.65 ALRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 PRR9 pKa = 11.84 TTRR12 pKa = 11.84 KK13 pKa = 9.58 RR14 pKa = 11.84 PARR17 pKa = 11.84 RR18 pKa = 11.84 SGGTTSRR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 YY28 pKa = 5.04 PTTRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 YY35 pKa = 7.54 PRR37 pKa = 11.84 KK38 pKa = 9.1 MSNKK42 pKa = 9.88 RR43 pKa = 11.84 ILNLTARR50 pKa = 11.84 KK51 pKa = 9.74 KK52 pKa = 10.39 RR53 pKa = 11.84 DD54 pKa = 3.14 TMQSFTNLSAAGGTATFAAQPAFLYY79 pKa = 10.61 GGRR82 pKa = 11.84 TYY84 pKa = 10.72 ILPWIATARR93 pKa = 11.84 DD94 pKa = 3.26 LSSNVNAPNTIAQEE108 pKa = 4.28 SARR111 pKa = 11.84 TATVCYY117 pKa = 9.75 MRR119 pKa = 11.84 GLKK122 pKa = 9.83 EE123 pKa = 4.19 RR124 pKa = 11.84 IQIQTSSGIPWQWRR138 pKa = 11.84 RR139 pKa = 11.84 ICFTVKK145 pKa = 10.68 GNDD148 pKa = 3.5 LLIGVNGDD156 pKa = 3.8 GDD158 pKa = 3.9 RR159 pKa = 11.84 QPLKK163 pKa = 9.3 EE164 pKa = 4.18 TTFGFMRR171 pKa = 11.84 NVVDD175 pKa = 3.91 WNDD178 pKa = 3.42 KK179 pKa = 10.63 ASAAIPLTEE188 pKa = 4.76 ALFKK192 pKa = 10.78 GQRR195 pKa = 11.84 NTDD198 pKa = 2.83 WRR200 pKa = 11.84 SLLNAPVDD208 pKa = 3.86 TRR210 pKa = 11.84 RR211 pKa = 11.84 VTLKK215 pKa = 10.19 YY216 pKa = 10.46 DD217 pKa = 3.12 KK218 pKa = 10.81 TRR220 pKa = 11.84 IISSGNSNGVLRR232 pKa = 11.84 NFNLWHH238 pKa = 6.72 PMNKK242 pKa = 9.55 NLVYY246 pKa = 10.83 GDD248 pKa = 4.69 DD249 pKa = 3.38 EE250 pKa = 4.78 GGNYY254 pKa = 9.76 DD255 pKa = 4.02 VEE257 pKa = 4.65 YY258 pKa = 11.15
Molecular weight: 29.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.251
IPC2_protein 10.043
IPC_protein 11.169
Toseland 11.242
ProMoST 11.55
Dawson 11.272
Bjellqvist 11.169
Wikipedia 11.652
Rodwell 11.242
Grimsley 11.33
Solomon 11.637
Lehninger 11.564
Nozaki 11.228
DTASelect 11.169
Thurlkill 11.242
EMBOSS 11.696
Sillero 11.242
Patrickios 10.935
IPC_peptide 11.652
IPC2_peptide 10.555
IPC2.peptide.svr19 8.885
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
600
258
342
300.0
34.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.0 ± 0.146
1.833 ± 0.625
6.833 ± 1.06
4.5 ± 1.284
4.0 ± 0.531
8.5 ± 0.671
2.167 ± 1.051
4.167 ± 0.286
4.5 ± 0.318
6.833 ± 0.314
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.833 ± 0.291
4.5 ± 1.691
5.167 ± 0.304
2.167 ± 0.552
9.833 ± 1.975
6.0 ± 0.11
6.833 ± 1.687
5.333 ± 0.632
2.833 ± 0.3
4.167 ± 0.401
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here