Pacific flying fox faeces associated circular DNA virus-13
Average proteome isoelectric point is 8.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140CTV9|A0A140CTV9_9VIRU Putative capsid protein OS=Pacific flying fox faeces associated circular DNA virus-13 OX=1796008 PE=4 SV=1
MM1 pKa = 7.33 SVHH4 pKa = 7.06 GNRR7 pKa = 11.84 AWCFTLNNPTDD18 pKa = 3.84 EE19 pKa = 4.69 EE20 pKa = 4.41 KK21 pKa = 11.15 LLLPTSPEE29 pKa = 4.16 SLPLWPRR36 pKa = 11.84 CTALVYY42 pKa = 10.43 QLEE45 pKa = 4.37 EE46 pKa = 4.59 GEE48 pKa = 4.55 NGTPHH53 pKa = 5.5 IQGYY57 pKa = 10.64 ARR59 pKa = 11.84 FDD61 pKa = 3.6 RR62 pKa = 11.84 QRR64 pKa = 11.84 TLATVKK70 pKa = 10.02 TILARR75 pKa = 11.84 AHH77 pKa = 6.24 WEE79 pKa = 3.7 PAKK82 pKa = 10.92 GSIQANLDD90 pKa = 3.43 YY91 pKa = 10.87 CGKK94 pKa = 9.58 QLGRR98 pKa = 11.84 LSEE101 pKa = 3.9 PVIIGFGGLRR111 pKa = 11.84 PGASGPSKK119 pKa = 9.99 PHH121 pKa = 6.56 LKK123 pKa = 10.02 RR124 pKa = 11.84 AQFVDD129 pKa = 6.11 LIASSPDD136 pKa = 3.13 VSIPEE141 pKa = 4.63 LLDD144 pKa = 3.28 QGALEE149 pKa = 4.36 VLATQPNLLGTLRR162 pKa = 11.84 GFLFQDD168 pKa = 3.76 LRR170 pKa = 11.84 RR171 pKa = 11.84 DD172 pKa = 3.69 GVIVDD177 pKa = 4.7 LYY179 pKa = 11.35 YY180 pKa = 11.39 GLTGTGKK187 pKa = 10.18 SRR189 pKa = 11.84 LAFDD193 pKa = 4.69 EE194 pKa = 4.39 YY195 pKa = 10.65 PGAFRR200 pKa = 11.84 KK201 pKa = 8.41 TSGSWWDD208 pKa = 3.48 SYY210 pKa = 11.65 AGEE213 pKa = 4.32 LSVVLDD219 pKa = 4.46 DD220 pKa = 5.71 FDD222 pKa = 5.04 DD223 pKa = 4.33 AFMPIGDD230 pKa = 4.98 LLRR233 pKa = 11.84 TLDD236 pKa = 4.09 RR237 pKa = 11.84 YY238 pKa = 9.12 PLRR241 pKa = 11.84 MPVKK245 pKa = 10.28 GGFIQLIANHH255 pKa = 6.76 FIITSNHH262 pKa = 6.22 LPSEE266 pKa = 4.88 WYY268 pKa = 9.87 PNAPPCRR275 pKa = 11.84 LAAVHH280 pKa = 6.49 RR281 pKa = 11.84 RR282 pKa = 11.84 FRR284 pKa = 11.84 NVVEE288 pKa = 5.0 FKK290 pKa = 10.68 DD291 pKa = 4.88 GYY293 pKa = 9.17 ITTHH297 pKa = 6.45 LASNYY302 pKa = 9.09 FNVGATSLTPGLTTPLPWTVTDD324 pKa = 4.09 RR325 pKa = 11.84 PVTPTQPYY333 pKa = 8.61 MSPEE337 pKa = 4.02 LTDD340 pKa = 3.46 SQLFATMDD348 pKa = 3.25
Molecular weight: 38.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.79
IPC2_protein 5.753
IPC_protein 5.804
Toseland 6.224
ProMoST 5.995
Dawson 6.071
Bjellqvist 6.084
Wikipedia 6.097
Rodwell 6.059
Grimsley 6.376
Solomon 6.084
Lehninger 6.071
Nozaki 6.338
DTASelect 6.532
Thurlkill 6.547
EMBOSS 6.517
Sillero 6.44
Patrickios 4.126
IPC_peptide 6.097
IPC2_peptide 6.44
IPC2.peptide.svr19 6.398
Protein with the highest isoelectric point:
>tr|A0A140CTW0|A0A140CTW0_9VIRU Uncharacterized protein OS=Pacific flying fox faeces associated circular DNA virus-13 OX=1796008 PE=4 SV=1
MM1 pKa = 7.54 PRR3 pKa = 11.84 FSRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 YY10 pKa = 8.92 RR11 pKa = 11.84 RR12 pKa = 11.84 PYY14 pKa = 9.16 RR15 pKa = 11.84 GRR17 pKa = 11.84 STRR20 pKa = 11.84 SRR22 pKa = 11.84 FRR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 LRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 FTGRR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.84 RR37 pKa = 11.84 YY38 pKa = 8.47 FVKK41 pKa = 10.41 KK42 pKa = 9.71 QLVPGSSVARR52 pKa = 11.84 IVMNTPIRR60 pKa = 11.84 ITLGTVADD68 pKa = 4.13 SRR70 pKa = 11.84 VGGAWIANSLEE81 pKa = 4.13 GPDD84 pKa = 5.18 FISSTPGLARR94 pKa = 11.84 GQYY97 pKa = 10.38 SIMRR101 pKa = 11.84 IAAGPSEE108 pKa = 4.67 TIPIGTAGATMYY120 pKa = 10.54 RR121 pKa = 11.84 QGHH124 pKa = 5.95 RR125 pKa = 11.84 DD126 pKa = 2.84 IAYY129 pKa = 8.8 VYY131 pKa = 10.5 SSNFMHH137 pKa = 7.61 LVRR140 pKa = 11.84 FYY142 pKa = 11.19 RR143 pKa = 11.84 NYY145 pKa = 10.57 RR146 pKa = 11.84 VVKK149 pKa = 7.84 TTLTLSLAYY158 pKa = 10.09 DD159 pKa = 3.66 NRR161 pKa = 11.84 PNSIDD166 pKa = 3.26 QTSGMSRR173 pKa = 11.84 FASVTLFATDD183 pKa = 5.26 DD184 pKa = 3.58 IDD186 pKa = 6.47 SIDD189 pKa = 3.67 TDD191 pKa = 4.38 GDD193 pKa = 3.96 INDD196 pKa = 3.19 KK197 pKa = 10.13 WIRR200 pKa = 11.84 PYY202 pKa = 11.02 AYY204 pKa = 9.62 GHH206 pKa = 5.84 RR207 pKa = 11.84 HH208 pKa = 5.19 VKK210 pKa = 9.36 TKK212 pKa = 9.43 TLHH215 pKa = 6.2 NGHH218 pKa = 7.15 ISPSKK223 pKa = 10.3 ISMSTSAYY231 pKa = 10.36 RR232 pKa = 11.84 LVKK235 pKa = 10.57 DD236 pKa = 3.5 RR237 pKa = 11.84 TTLTSADD244 pKa = 3.8 YY245 pKa = 10.61 VGSITPVEE253 pKa = 4.39 PQPPIYY259 pKa = 9.99 PGLNSPEE266 pKa = 3.92 KK267 pKa = 9.72 KK268 pKa = 10.4 VYY270 pKa = 9.62 IGFVISPYY278 pKa = 10.53 GVPTGGWPASTEE290 pKa = 4.0 IVSGYY295 pKa = 8.81 LTVTQKK301 pKa = 10.42 VQFFNPHH308 pKa = 5.93 DD309 pKa = 3.57 TADD312 pKa = 3.66 FYY314 pKa = 11.91 GPVV317 pKa = 3.15
Molecular weight: 35.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 10.072
IPC_protein 10.921
Toseland 10.628
ProMoST 10.511
Dawson 10.804
Bjellqvist 10.584
Wikipedia 11.082
Rodwell 10.891
Grimsley 10.891
Solomon 10.921
Lehninger 10.862
Nozaki 10.599
DTASelect 10.584
Thurlkill 10.672
EMBOSS 11.052
Sillero 10.73
Patrickios 10.496
IPC_peptide 10.906
IPC2_peptide 9.604
IPC2.peptide.svr19 8.127
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
776
111
348
258.7
29.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.572 ± 0.511
0.515 ± 0.346
5.284 ± 0.504
3.351 ± 0.916
4.124 ± 0.383
7.216 ± 1.133
2.32 ± 0.099
4.768 ± 1.26
3.866 ± 0.877
9.278 ± 2.275
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.933 ± 0.29
3.866 ± 0.841
6.701 ± 1.507
3.479 ± 0.994
9.021 ± 1.474
7.088 ± 1.289
7.99 ± 0.516
5.67 ± 0.712
2.062 ± 0.887
4.897 ± 0.794
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here