Proteus phage vB_PmiP_RS1pmA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Novosibovirus; unclassified Novosibovirus

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514CY23|A0A514CY23_9CAUD Uncharacterized protein OS=Proteus phage vB_PmiP_RS1pmA OX=2250312 PE=4 SV=1
MM1 pKa = 7.33IKK3 pKa = 10.31FYY5 pKa = 10.53EE6 pKa = 4.59FPSTDD11 pKa = 3.17TLIVLDD17 pKa = 4.33TEE19 pKa = 4.34KK20 pKa = 10.82QLAALFPLAYY30 pKa = 9.57IKK32 pKa = 10.8GRR34 pKa = 11.84YY35 pKa = 8.65NDD37 pKa = 4.24LNTWWGDD44 pKa = 3.51YY45 pKa = 10.62DD46 pKa = 3.26ISEE49 pKa = 4.42YY50 pKa = 10.86VVSNFEE56 pKa = 4.25GVLDD60 pKa = 3.96SLKK63 pKa = 10.81DD64 pKa = 3.52YY65 pKa = 11.04YY66 pKa = 11.6AA67 pKa = 5.3

Molecular weight: 7.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514CY28|A0A514CY28_9CAUD Uncharacterized protein OS=Proteus phage vB_PmiP_RS1pmA OX=2250312 PE=4 SV=1
MM1 pKa = 7.99RR2 pKa = 11.84SNKK5 pKa = 9.47KK6 pKa = 10.59YY7 pKa = 9.89KK8 pKa = 10.48GKK10 pKa = 8.35YY11 pKa = 5.71TKK13 pKa = 10.2KK14 pKa = 10.25RR15 pKa = 11.84LEE17 pKa = 3.63NLIRR21 pKa = 11.84GKK23 pKa = 10.0MYY25 pKa = 10.78FMVKK29 pKa = 7.14THH31 pKa = 6.36NFPSYY36 pKa = 9.07RR37 pKa = 11.84KK38 pKa = 10.47AMIAYY43 pKa = 7.01EE44 pKa = 4.42WYY46 pKa = 10.17RR47 pKa = 11.84EE48 pKa = 4.02GKK50 pKa = 8.12ITFDD54 pKa = 4.98QFLQWGYY61 pKa = 11.45

Molecular weight: 7.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12811

43

1257

241.7

27.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.275 ± 0.503

1.132 ± 0.198

5.651 ± 0.205

6.096 ± 0.338

3.427 ± 0.195

6.846 ± 0.27

1.897 ± 0.179

5.331 ± 0.274

6.182 ± 0.358

9.203 ± 0.338

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.716 ± 0.179

4.348 ± 0.212

3.544 ± 0.256

5.011 ± 0.396

4.707 ± 0.297

7.47 ± 0.318

6.775 ± 0.232

7.01 ± 0.291

1.265 ± 0.096

4.114 ± 0.266
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski