Amino acid dipepetide frequency for Shigella phage MK-13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.17AlaAla: 6.17 ± 0.452
0.683AlaCys: 0.683 ± 0.095
4.596AlaAsp: 4.596 ± 0.335
4.679AlaGlu: 4.679 ± 0.408
2.588AlaPhe: 2.588 ± 0.245
5.093AlaGly: 5.093 ± 0.39
1.449AlaHis: 1.449 ± 0.179
4.348AlaIle: 4.348 ± 0.263
4.555AlaLys: 4.555 ± 0.286
6.19AlaLeu: 6.19 ± 0.345
2.05AlaMet: 2.05 ± 0.207
3.064AlaAsn: 3.064 ± 0.26
2.671AlaPro: 2.671 ± 0.288
2.961AlaGln: 2.961 ± 0.276
3.561AlaArg: 3.561 ± 0.289
4.306AlaSer: 4.306 ± 0.358
4.617AlaThr: 4.617 ± 0.363
5.155AlaVal: 5.155 ± 0.284
0.973AlaTrp: 0.973 ± 0.139
2.484AlaTyr: 2.484 ± 0.206
0.0AlaXaa: 0.0 ± 0.0
Cys
0.787CysAla: 0.787 ± 0.122
0.145CysCys: 0.145 ± 0.058
0.807CysAsp: 0.807 ± 0.134
0.766CysGlu: 0.766 ± 0.133
0.373CysPhe: 0.373 ± 0.08
0.725CysGly: 0.725 ± 0.119
0.29CysHis: 0.29 ± 0.08
0.663CysIle: 0.663 ± 0.111
0.725CysLys: 0.725 ± 0.12
0.745CysLeu: 0.745 ± 0.134
0.207CysMet: 0.207 ± 0.069
0.621CysAsn: 0.621 ± 0.093
0.497CysPro: 0.497 ± 0.088
0.311CysGln: 0.311 ± 0.078
0.476CysArg: 0.476 ± 0.091
0.89CysSer: 0.89 ± 0.145
0.538CysThr: 0.538 ± 0.096
1.014CysVal: 1.014 ± 0.143
0.166CysTrp: 0.166 ± 0.063
0.414CysTyr: 0.414 ± 0.099
0.0CysXaa: 0.0 ± 0.0
Asp
4.948AspAla: 4.948 ± 0.377
0.642AspCys: 0.642 ± 0.114
4.224AspAsp: 4.224 ± 0.359
4.099AspGlu: 4.099 ± 0.288
3.23AspPhe: 3.23 ± 0.246
5.197AspGly: 5.197 ± 0.3
0.911AspHis: 0.911 ± 0.13
4.679AspIle: 4.679 ± 0.279
3.623AspLys: 3.623 ± 0.29
5.652AspLeu: 5.652 ± 0.376
2.05AspMet: 2.05 ± 0.227
2.65AspAsn: 2.65 ± 0.261
2.94AspPro: 2.94 ± 0.225
2.174AspGln: 2.174 ± 0.204
2.816AspArg: 2.816 ± 0.271
3.271AspSer: 3.271 ± 0.25
3.25AspThr: 3.25 ± 0.25
4.783AspVal: 4.783 ± 0.296
0.994AspTrp: 0.994 ± 0.136
3.499AspTyr: 3.499 ± 0.271
0.0AspXaa: 0.0 ± 0.0
Glu
4.679GluAla: 4.679 ± 0.433
0.683GluCys: 0.683 ± 0.121
4.058GluAsp: 4.058 ± 0.363
4.555GluGlu: 4.555 ± 0.405
2.94GluPhe: 2.94 ± 0.23
4.431GluGly: 4.431 ± 0.302
1.325GluHis: 1.325 ± 0.189
3.996GluIle: 3.996 ± 0.271
3.354GluLys: 3.354 ± 0.322
6.377GluLeu: 6.377 ± 0.354
2.174GluMet: 2.174 ± 0.186
2.795GluAsn: 2.795 ± 0.259
2.029GluPro: 2.029 ± 0.203
2.526GluGln: 2.526 ± 0.197
3.52GluArg: 3.52 ± 0.252
3.25GluSer: 3.25 ± 0.298
3.727GluThr: 3.727 ± 0.24
4.969GluVal: 4.969 ± 0.327
1.159GluTrp: 1.159 ± 0.151
2.878GluTyr: 2.878 ± 0.246
0.0GluXaa: 0.0 ± 0.0
Phe
2.422PheAla: 2.422 ± 0.238
0.311PheCys: 0.311 ± 0.09
2.691PheAsp: 2.691 ± 0.264
3.395PheGlu: 3.395 ± 0.252
1.718PhePhe: 1.718 ± 0.184
2.94PheGly: 2.94 ± 0.249
0.87PheHis: 0.87 ± 0.135
2.816PheIle: 2.816 ± 0.239
2.567PheLys: 2.567 ± 0.225
3.147PheLeu: 3.147 ± 0.244
1.325PheMet: 1.325 ± 0.17
2.443PheAsn: 2.443 ± 0.257
1.594PhePro: 1.594 ± 0.171
1.698PheGln: 1.698 ± 0.185
2.153PheArg: 2.153 ± 0.202
3.002PheSer: 3.002 ± 0.277
2.836PheThr: 2.836 ± 0.231
2.691PheVal: 2.691 ± 0.219
0.621PheTrp: 0.621 ± 0.11
1.636PheTyr: 1.636 ± 0.177
0.0PheXaa: 0.0 ± 0.0
Gly
4.431GlyAla: 4.431 ± 0.354
0.994GlyCys: 0.994 ± 0.147
3.809GlyAsp: 3.809 ± 0.24
4.534GlyGlu: 4.534 ± 0.322
3.064GlyPhe: 3.064 ± 0.288
5.031GlyGly: 5.031 ± 0.443
1.553GlyHis: 1.553 ± 0.192
4.762GlyIle: 4.762 ± 0.305
4.783GlyLys: 4.783 ± 0.345
5.217GlyLeu: 5.217 ± 0.255
2.029GlyMet: 2.029 ± 0.206
3.561GlyAsn: 3.561 ± 0.303
1.18GlyPro: 1.18 ± 0.142
2.733GlyGln: 2.733 ± 0.224
3.168GlyArg: 3.168 ± 0.238
4.348GlySer: 4.348 ± 0.368
4.182GlyThr: 4.182 ± 0.407
5.611GlyVal: 5.611 ± 0.347
1.263GlyTrp: 1.263 ± 0.185
2.36GlyTyr: 2.36 ± 0.243
0.0GlyXaa: 0.0 ± 0.0
His
1.242HisAla: 1.242 ± 0.183
0.311HisCys: 0.311 ± 0.074
1.242HisAsp: 1.242 ± 0.174
0.725HisGlu: 0.725 ± 0.113
0.911HisPhe: 0.911 ± 0.14
1.118HisGly: 1.118 ± 0.16
0.435HisHis: 0.435 ± 0.094
1.429HisIle: 1.429 ± 0.229
1.18HisLys: 1.18 ± 0.152
1.511HisLeu: 1.511 ± 0.176
0.642HisMet: 0.642 ± 0.101
0.766HisAsn: 0.766 ± 0.119
0.911HisPro: 0.911 ± 0.147
0.663HisGln: 0.663 ± 0.124
1.056HisArg: 1.056 ± 0.166
1.097HisSer: 1.097 ± 0.184
1.222HisThr: 1.222 ± 0.179
1.077HisVal: 1.077 ± 0.186
0.228HisTrp: 0.228 ± 0.068
0.787HisTyr: 0.787 ± 0.138
0.0HisXaa: 0.0 ± 0.0
Ile
4.389IleAla: 4.389 ± 0.305
0.621IleCys: 0.621 ± 0.12
5.362IleAsp: 5.362 ± 0.35
4.803IleGlu: 4.803 ± 0.324
1.863IlePhe: 1.863 ± 0.184
3.768IleGly: 3.768 ± 0.243
1.222IleHis: 1.222 ± 0.186
3.354IleIle: 3.354 ± 0.297
3.582IleLys: 3.582 ± 0.251
3.975IleLeu: 3.975 ± 0.282
1.408IleMet: 1.408 ± 0.201
3.354IleAsn: 3.354 ± 0.264
2.754IlePro: 2.754 ± 0.23
2.733IleGln: 2.733 ± 0.255
3.271IleArg: 3.271 ± 0.25
3.271IleSer: 3.271 ± 0.289
4.368IleThr: 4.368 ± 0.279
4.037IleVal: 4.037 ± 0.292
0.642IleTrp: 0.642 ± 0.125
1.698IleTyr: 1.698 ± 0.19
0.0IleXaa: 0.0 ± 0.0
Lys
4.079LysAla: 4.079 ± 0.348
0.559LysCys: 0.559 ± 0.123
4.348LysAsp: 4.348 ± 0.294
4.224LysGlu: 4.224 ± 0.347
2.857LysPhe: 2.857 ± 0.227
4.161LysGly: 4.161 ± 0.318
1.097LysHis: 1.097 ± 0.163
3.665LysIle: 3.665 ± 0.258
4.244LysLys: 4.244 ± 0.384
5.259LysLeu: 5.259 ± 0.325
2.629LysMet: 2.629 ± 0.282
2.671LysAsn: 2.671 ± 0.227
2.547LysPro: 2.547 ± 0.235
2.464LysGln: 2.464 ± 0.214
3.209LysArg: 3.209 ± 0.31
4.099LysSer: 4.099 ± 0.282
3.437LysThr: 3.437 ± 0.26
4.493LysVal: 4.493 ± 0.356
0.87LysTrp: 0.87 ± 0.124
2.112LysTyr: 2.112 ± 0.205
0.0LysXaa: 0.0 ± 0.0
Leu
6.356LeuAla: 6.356 ± 0.365
0.787LeuCys: 0.787 ± 0.132
5.383LeuAsp: 5.383 ± 0.36
5.052LeuGlu: 5.052 ± 0.329
3.313LeuPhe: 3.313 ± 0.288
5.155LeuGly: 5.155 ± 0.335
1.429LeuHis: 1.429 ± 0.169
4.141LeuIle: 4.141 ± 0.302
6.356LeuLys: 6.356 ± 0.374
6.439LeuLeu: 6.439 ± 0.475
2.174LeuMet: 2.174 ± 0.19
4.203LeuAsn: 4.203 ± 0.331
3.768LeuPro: 3.768 ± 0.3
2.878LeuGln: 2.878 ± 0.268
3.954LeuArg: 3.954 ± 0.274
5.342LeuSer: 5.342 ± 0.342
5.093LeuThr: 5.093 ± 0.325
5.838LeuVal: 5.838 ± 0.377
0.787LeuTrp: 0.787 ± 0.14
3.188LeuTyr: 3.188 ± 0.271
0.0LeuXaa: 0.0 ± 0.0
Met
2.65MetAla: 2.65 ± 0.25
0.331MetCys: 0.331 ± 0.109
1.636MetAsp: 1.636 ± 0.189
1.304MetGlu: 1.304 ± 0.174
1.615MetPhe: 1.615 ± 0.196
1.698MetGly: 1.698 ± 0.207
0.435MetHis: 0.435 ± 0.107
1.511MetIle: 1.511 ± 0.156
2.277MetLys: 2.277 ± 0.196
2.36MetLeu: 2.36 ± 0.222
0.807MetMet: 0.807 ± 0.119
1.532MetAsn: 1.532 ± 0.172
0.994MetPro: 0.994 ± 0.154
1.014MetGln: 1.014 ± 0.148
1.594MetArg: 1.594 ± 0.175
2.277MetSer: 2.277 ± 0.202
1.698MetThr: 1.698 ± 0.175
1.925MetVal: 1.925 ± 0.185
0.311MetTrp: 0.311 ± 0.088
0.911MetTyr: 0.911 ± 0.144
0.0MetXaa: 0.0 ± 0.0
Asn
3.83AsnAla: 3.83 ± 0.287
0.663AsnCys: 0.663 ± 0.159
2.443AsnAsp: 2.443 ± 0.209
2.464AsnGlu: 2.464 ± 0.244
2.05AsnPhe: 2.05 ± 0.23
4.224AsnGly: 4.224 ± 0.356
0.973AsnHis: 0.973 ± 0.186
3.023AsnIle: 3.023 ± 0.291
2.754AsnLys: 2.754 ± 0.186
3.934AsnLeu: 3.934 ± 0.304
1.387AsnMet: 1.387 ± 0.166
2.774AsnAsn: 2.774 ± 0.304
2.319AsnPro: 2.319 ± 0.238
1.677AsnGln: 1.677 ± 0.18
2.339AsnArg: 2.339 ± 0.227
2.422AsnSer: 2.422 ± 0.234
3.023AsnThr: 3.023 ± 0.27
3.561AsnVal: 3.561 ± 0.243
0.621AsnTrp: 0.621 ± 0.117
1.801AsnTyr: 1.801 ± 0.208
0.0AsnXaa: 0.0 ± 0.0
Pro
2.671ProAla: 2.671 ± 0.235
0.435ProCys: 0.435 ± 0.079
3.064ProAsp: 3.064 ± 0.252
3.333ProGlu: 3.333 ± 0.281
1.884ProPhe: 1.884 ± 0.226
2.36ProGly: 2.36 ± 0.227
0.621ProHis: 0.621 ± 0.106
1.925ProIle: 1.925 ± 0.188
2.298ProLys: 2.298 ± 0.212
2.816ProLeu: 2.816 ± 0.212
0.952ProMet: 0.952 ± 0.134
1.698ProAsn: 1.698 ± 0.196
1.284ProPro: 1.284 ± 0.201
1.553ProGln: 1.553 ± 0.182
1.677ProArg: 1.677 ± 0.184
3.002ProSer: 3.002 ± 0.24
2.629ProThr: 2.629 ± 0.243
3.147ProVal: 3.147 ± 0.265
0.559ProTrp: 0.559 ± 0.095
1.325ProTyr: 1.325 ± 0.155
0.0ProXaa: 0.0 ± 0.0
Gln
2.981GlnAla: 2.981 ± 0.338
0.414GlnCys: 0.414 ± 0.088
2.526GlnAsp: 2.526 ± 0.213
2.36GlnGlu: 2.36 ± 0.295
2.091GlnPhe: 2.091 ± 0.231
2.402GlnGly: 2.402 ± 0.192
0.642GlnHis: 0.642 ± 0.117
2.195GlnIle: 2.195 ± 0.224
2.277GlnLys: 2.277 ± 0.194
3.002GlnLeu: 3.002 ± 0.214
1.18GlnMet: 1.18 ± 0.126
1.594GlnAsn: 1.594 ± 0.184
1.263GlnPro: 1.263 ± 0.153
1.801GlnGln: 1.801 ± 0.29
1.76GlnArg: 1.76 ± 0.175
2.195GlnSer: 2.195 ± 0.203
2.277GlnThr: 2.277 ± 0.216
2.733GlnVal: 2.733 ± 0.214
0.6GlnTrp: 0.6 ± 0.104
1.594GlnTyr: 1.594 ± 0.166
0.0GlnXaa: 0.0 ± 0.0
Arg
3.126ArgAla: 3.126 ± 0.209
0.683ArgCys: 0.683 ± 0.14
3.043ArgAsp: 3.043 ± 0.233
3.209ArgGlu: 3.209 ± 0.262
2.091ArgPhe: 2.091 ± 0.227
3.147ArgGly: 3.147 ± 0.223
1.035ArgHis: 1.035 ± 0.131
3.188ArgIle: 3.188 ± 0.25
3.147ArgLys: 3.147 ± 0.27
4.679ArgLeu: 4.679 ± 0.3
1.739ArgMet: 1.739 ± 0.2
2.277ArgAsn: 2.277 ± 0.237
1.822ArgPro: 1.822 ± 0.216
1.843ArgGln: 1.843 ± 0.186
3.147ArgArg: 3.147 ± 0.308
2.588ArgSer: 2.588 ± 0.219
2.588ArgThr: 2.588 ± 0.231
3.23ArgVal: 3.23 ± 0.219
0.725ArgTrp: 0.725 ± 0.14
2.236ArgTyr: 2.236 ± 0.242
0.0ArgXaa: 0.0 ± 0.0
Ser
3.872SerAla: 3.872 ± 0.319
0.476SerCys: 0.476 ± 0.128
3.54SerAsp: 3.54 ± 0.238
3.52SerGlu: 3.52 ± 0.228
2.609SerPhe: 2.609 ± 0.246
4.845SerGly: 4.845 ± 0.536
0.849SerHis: 0.849 ± 0.145
4.079SerIle: 4.079 ± 0.262
3.457SerLys: 3.457 ± 0.275
5.3SerLeu: 5.3 ± 0.363
1.573SerMet: 1.573 ± 0.18
3.457SerAsn: 3.457 ± 0.325
2.422SerPro: 2.422 ± 0.203
2.277SerGln: 2.277 ± 0.221
2.795SerArg: 2.795 ± 0.208
4.037SerSer: 4.037 ± 0.362
3.437SerThr: 3.437 ± 0.356
4.534SerVal: 4.534 ± 0.322
0.58SerTrp: 0.58 ± 0.106
2.484SerTyr: 2.484 ± 0.255
0.0SerXaa: 0.0 ± 0.0
Thr
4.327ThrAla: 4.327 ± 0.371
0.683ThrCys: 0.683 ± 0.113
3.582ThrAsp: 3.582 ± 0.31
4.058ThrGlu: 4.058 ± 0.286
2.857ThrPhe: 2.857 ± 0.256
4.513ThrGly: 4.513 ± 0.336
1.014ThrHis: 1.014 ± 0.162
3.809ThrIle: 3.809 ± 0.261
3.768ThrLys: 3.768 ± 0.3
5.238ThrLeu: 5.238 ± 0.333
1.159ThrMet: 1.159 ± 0.16
2.588ThrAsn: 2.588 ± 0.279
3.727ThrPro: 3.727 ± 0.263
1.905ThrGln: 1.905 ± 0.246
3.085ThrArg: 3.085 ± 0.257
2.94ThrSer: 2.94 ± 0.306
3.872ThrThr: 3.872 ± 0.358
4.907ThrVal: 4.907 ± 0.39
0.807ThrTrp: 0.807 ± 0.135
1.884ThrTyr: 1.884 ± 0.223
0.0ThrXaa: 0.0 ± 0.0
Val
5.072ValAla: 5.072 ± 0.352
0.952ValCys: 0.952 ± 0.131
5.549ValAsp: 5.549 ± 0.313
5.093ValGlu: 5.093 ± 0.396
2.712ValPhe: 2.712 ± 0.256
4.513ValGly: 4.513 ± 0.284
1.159ValHis: 1.159 ± 0.15
4.099ValIle: 4.099 ± 0.257
5.238ValLys: 5.238 ± 0.4
5.424ValLeu: 5.424 ± 0.356
2.029ValMet: 2.029 ± 0.23
3.437ValAsn: 3.437 ± 0.283
2.65ValPro: 2.65 ± 0.201
2.795ValGln: 2.795 ± 0.267
3.209ValArg: 3.209 ± 0.246
4.658ValSer: 4.658 ± 0.359
5.114ValThr: 5.114 ± 0.459
6.273ValVal: 6.273 ± 0.448
1.118ValTrp: 1.118 ± 0.148
2.94ValTyr: 2.94 ± 0.254
0.0ValXaa: 0.0 ± 0.0
Trp
1.118TrpAla: 1.118 ± 0.18
0.269TrpCys: 0.269 ± 0.082
0.973TrpAsp: 0.973 ± 0.157
1.201TrpGlu: 1.201 ± 0.155
0.642TrpPhe: 0.642 ± 0.104
0.787TrpGly: 0.787 ± 0.128
0.207TrpHis: 0.207 ± 0.065
0.745TrpIle: 0.745 ± 0.122
0.828TrpLys: 0.828 ± 0.133
1.304TrpLeu: 1.304 ± 0.15
0.352TrpMet: 0.352 ± 0.09
0.787TrpAsn: 0.787 ± 0.134
0.393TrpPro: 0.393 ± 0.089
0.311TrpGln: 0.311 ± 0.078
0.89TrpArg: 0.89 ± 0.171
0.704TrpSer: 0.704 ± 0.135
0.642TrpThr: 0.642 ± 0.113
0.973TrpVal: 0.973 ± 0.145
0.166TrpTrp: 0.166 ± 0.046
0.435TrpTyr: 0.435 ± 0.096
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.043TyrAla: 3.043 ± 0.256
0.518TyrCys: 0.518 ± 0.107
2.754TyrAsp: 2.754 ± 0.191
2.05TyrGlu: 2.05 ± 0.204
1.47TyrPhe: 1.47 ± 0.154
2.505TyrGly: 2.505 ± 0.228
1.035TyrHis: 1.035 ± 0.157
2.05TyrIle: 2.05 ± 0.213
2.008TyrLys: 2.008 ± 0.211
3.064TyrLeu: 3.064 ± 0.246
0.994TyrMet: 0.994 ± 0.147
2.008TyrAsn: 2.008 ± 0.191
1.47TyrPro: 1.47 ± 0.152
1.573TyrGln: 1.573 ± 0.208
1.863TyrArg: 1.863 ± 0.187
2.464TyrSer: 2.464 ± 0.23
2.174TyrThr: 2.174 ± 0.249
3.085TyrVal: 3.085 ± 0.236
0.538TyrTrp: 0.538 ± 0.115
1.304TyrTyr: 1.304 ± 0.141
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 207 proteins (48302 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski