Human immunodeficiency virus type 1 group M subtype B (isolate YU-2) (HIV-1)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 unknown group

Average proteome isoelectric point is 7.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P35967|VPR_HV1Y2 Protein Vpr OS=Human immunodeficiency virus type 1 group M subtype B (isolate YU-2) OX=362651 GN=vpr PE=1 SV=1
LL1 pKa = 7.73QSLQVLAIVALVVATIIAIVVWTIVFIEE29 pKa = 3.94YY30 pKa = 10.37RR31 pKa = 11.84KK32 pKa = 9.66ILRR35 pKa = 11.84QRR37 pKa = 11.84KK38 pKa = 8.13IDD40 pKa = 3.6RR41 pKa = 11.84LINRR45 pKa = 11.84ITEE48 pKa = 4.0RR49 pKa = 11.84AEE51 pKa = 4.15DD52 pKa = 3.83SGNEE56 pKa = 3.81SDD58 pKa = 5.31GDD60 pKa = 3.92QEE62 pKa = 4.11EE63 pKa = 4.6LSALVEE69 pKa = 4.4RR70 pKa = 11.84GHH72 pKa = 6.72LAPWDD77 pKa = 3.77VDD79 pKa = 3.88DD80 pKa = 5.48LL81 pKa = 4.6

Molecular weight: 9.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P35961|ENV_HV1Y2 Envelope glycoprotein gp160 OS=Human immunodeficiency virus type 1 group M subtype B (isolate YU-2) OX=362651 GN=env PE=1 SV=1
MM1 pKa = 7.87AGRR4 pKa = 11.84SGDD7 pKa = 3.51SDD9 pKa = 3.88EE10 pKa = 5.47DD11 pKa = 3.59LLRR14 pKa = 11.84TVRR17 pKa = 11.84LIKK20 pKa = 10.36VLYY23 pKa = 9.78QSNPPPSSEE32 pKa = 3.72GTRR35 pKa = 11.84QARR38 pKa = 11.84RR39 pKa = 11.84NRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84WRR46 pKa = 11.84EE47 pKa = 3.07RR48 pKa = 11.84QRR50 pKa = 11.84QIRR53 pKa = 11.84SISGWLLSNYY63 pKa = 9.62LGRR66 pKa = 11.84PTEE69 pKa = 4.05PVPFQLPPLEE79 pKa = 5.3RR80 pKa = 11.84LTLDD84 pKa = 3.67CNEE87 pKa = 4.63DD88 pKa = 3.78CGTSGTQGVGSPQILVEE105 pKa = 4.4SPPVLDD111 pKa = 4.63SGTKK115 pKa = 8.64EE116 pKa = 3.73

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

3651

72

1435

365.1

41.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.354 ± 0.573

2.027 ± 0.426

3.972 ± 0.287

7.149 ± 0.548

2.465 ± 0.13

7.121 ± 0.211

2.356 ± 0.481

6.327 ± 0.688

6.875 ± 0.849

8.354 ± 0.556

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.054 ± 0.225

4.382 ± 0.604

5.423 ± 0.549

6.135 ± 0.389

6.354 ± 0.871

5.314 ± 0.563

6.108 ± 0.319

5.971 ± 0.402

2.712 ± 0.246

2.547 ± 0.218
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski