Capnocytophaga sp. oral taxon 332 str. F0381
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2698 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L1P0F1|L1P0F1_9FLAO Transporter small conductance mechanosensitive ion channel MscS family protein OS=Capnocytophaga sp. oral taxon 332 str. F0381 OX=1127692 GN=HMPREF9075_01549 PE=3 SV=1
MM1 pKa = 6.7 NTIVLKK7 pKa = 11.09 NSINYY12 pKa = 8.43 HH13 pKa = 5.25 QYY15 pKa = 9.57 QQIADD20 pKa = 4.33 FFATIGVEE28 pKa = 3.8 IANSYY33 pKa = 11.31 GEE35 pKa = 4.24 VTLQDD40 pKa = 4.15 DD41 pKa = 4.08 EE42 pKa = 5.52 ALLEE46 pKa = 4.27 ALDD49 pKa = 3.94 MGITQADD56 pKa = 3.57 NGLLIPHH63 pKa = 6.57 NEE65 pKa = 3.59 VMQEE69 pKa = 3.32 IDD71 pKa = 4.01 SLIKK75 pKa = 10.44 SYY77 pKa = 11.22 QKK79 pKa = 11.2
Molecular weight: 8.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.84
IPC2_protein 4.113
IPC_protein 3.986
Toseland 3.808
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.126
Wikipedia 3.884
Rodwell 3.821
Grimsley 3.732
Solomon 3.948
Lehninger 3.897
Nozaki 4.101
DTASelect 4.266
Thurlkill 3.859
EMBOSS 3.897
Sillero 4.113
Patrickios 1.926
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 3.996
Protein with the highest isoelectric point:
>tr|L1P421|L1P421_9FLAO Biotin--[acetyl-CoA-carboxylase] ligase OS=Capnocytophaga sp. oral taxon 332 str. F0381 OX=1127692 GN=HMPREF9075_01147 PE=4 SV=1
MM1 pKa = 7.55 HH2 pKa = 7.33 FGKK5 pKa = 10.74 GYY7 pKa = 10.26 GNRR10 pKa = 11.84 STAFAKK16 pKa = 10.64 VFFTSHH22 pKa = 6.15 ISLITTEE29 pKa = 4.35 PSAKK33 pKa = 9.73 VEE35 pKa = 4.01 RR36 pKa = 11.84 RR37 pKa = 11.84 LSEE40 pKa = 4.09 GKK42 pKa = 9.38 SASWRR47 pKa = 11.84 SRR49 pKa = 11.84 VCEE52 pKa = 3.57 ARR54 pKa = 11.84 AARR57 pKa = 11.84 TEE59 pKa = 4.16 GKK61 pKa = 9.69 SVSWRR66 pKa = 11.84 SQVCEE71 pKa = 3.66 ARR73 pKa = 11.84 AAHH76 pKa = 6.08 TNSKK80 pKa = 10.13 SLPITFLSNKK90 pKa = 9.24 INYY93 pKa = 8.42
Molecular weight: 10.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.663
IPC_protein 10.189
Toseland 10.745
ProMoST 10.365
Dawson 10.833
Bjellqvist 10.482
Wikipedia 10.994
Rodwell 11.169
Grimsley 10.862
Solomon 10.921
Lehninger 10.906
Nozaki 10.73
DTASelect 10.482
Thurlkill 10.73
EMBOSS 11.14
Sillero 10.76
Patrickios 10.935
IPC_peptide 10.935
IPC2_peptide 9.443
IPC2.peptide.svr19 8.604
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2698
0
2698
810813
39
4121
300.5
34.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.081 ± 0.054
0.887 ± 0.018
5.057 ± 0.039
6.705 ± 0.054
4.826 ± 0.038
6.113 ± 0.056
1.84 ± 0.022
7.448 ± 0.052
7.653 ± 0.06
9.35 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.126 ± 0.024
5.669 ± 0.041
3.605 ± 0.029
3.903 ± 0.034
3.844 ± 0.026
5.683 ± 0.038
6.185 ± 0.083
6.373 ± 0.045
1.041 ± 0.017
4.61 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here