Bacteroides stercoris
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3155 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A108TAS8|A0A108TAS8_BACSE Trk system potassium uptake protein TrkA OS=Bacteroides stercoris OX=46506 GN=trkA PE=4 SV=1
MM1 pKa = 7.32 GLEE4 pKa = 5.16 DD5 pKa = 5.97 DD6 pKa = 4.6 FLKK9 pKa = 11.1 NDD11 pKa = 4.18 LDD13 pKa = 4.02 DD14 pKa = 4.52 EE15 pKa = 4.42 KK16 pKa = 10.6 TIEE19 pKa = 4.18 YY20 pKa = 9.37 IKK22 pKa = 10.96 NYY24 pKa = 9.95 LPQEE28 pKa = 4.16 LKK30 pKa = 10.91 EE31 pKa = 4.3 KK32 pKa = 10.78 FSDD35 pKa = 3.62 DD36 pKa = 3.07 EE37 pKa = 4.59 FYY39 pKa = 11.59 YY40 pKa = 10.87 FLDD43 pKa = 5.8 LIDD46 pKa = 4.53 EE47 pKa = 4.8 YY48 pKa = 11.34 YY49 pKa = 10.34 SEE51 pKa = 5.1 SGILDD56 pKa = 3.6 VQPDD60 pKa = 3.61 GDD62 pKa = 4.32 GYY64 pKa = 10.85 IEE66 pKa = 4.74 IDD68 pKa = 3.09 LGKK71 pKa = 10.24 IVDD74 pKa = 4.12 YY75 pKa = 10.58 IIKK78 pKa = 9.46 EE79 pKa = 3.92 AHH81 pKa = 6.35 KK82 pKa = 10.98 DD83 pKa = 3.53 EE84 pKa = 4.7 MGDD87 pKa = 3.65 YY88 pKa = 11.01 DD89 pKa = 4.15 PDD91 pKa = 4.14 EE92 pKa = 4.41 ILFIVQGEE100 pKa = 4.27 MEE102 pKa = 4.35 YY103 pKa = 10.46 TEE105 pKa = 4.41 SLEE108 pKa = 5.06 DD109 pKa = 3.48 EE110 pKa = 4.5
Molecular weight: 13.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.63
IPC_protein 3.617
Toseland 3.401
ProMoST 3.757
Dawson 3.605
Bjellqvist 3.795
Wikipedia 3.541
Rodwell 3.452
Grimsley 3.312
Solomon 3.592
Lehninger 3.554
Nozaki 3.719
DTASelect 3.935
Thurlkill 3.465
EMBOSS 3.541
Sillero 3.732
Patrickios 0.985
IPC_peptide 3.592
IPC2_peptide 3.719
IPC2.peptide.svr19 3.72
Protein with the highest isoelectric point:
>tr|A0A120A0X1|A0A120A0X1_BACSE AAA-like domain protein OS=Bacteroides stercoris OX=46506 GN=AA415_02743 PE=4 SV=1
MM1 pKa = 7.71 LIAHH5 pKa = 6.26 CQFLLFSSVLLAFFLCPTPGCRR27 pKa = 11.84 AYY29 pKa = 10.57 RR30 pKa = 11.84 DD31 pKa = 3.56 PGVRR35 pKa = 11.84 RR36 pKa = 11.84 AEE38 pKa = 3.92 TRR40 pKa = 11.84 MSDD43 pKa = 3.26 MSRR46 pKa = 11.84 PGCHH50 pKa = 6.58 AMPFRR55 pKa = 11.84 ISVFSFSLPLFLSARR70 pKa = 11.84 MRR72 pKa = 11.84 IYY74 pKa = 11.19 YY75 pKa = 9.7 NVSLLPGFPSSLKK88 pKa = 10.92 NMFFNIFVNQRR99 pKa = 11.84 FIKK102 pKa = 9.51 MFKK105 pKa = 10.35 RR106 pKa = 11.84 NAKK109 pKa = 8.97 KK110 pKa = 9.93 SPYY113 pKa = 9.4 IFGVYY118 pKa = 9.55 VIKK121 pKa = 10.68 SYY123 pKa = 10.57 FCTRR127 pKa = 11.84 FRR129 pKa = 11.84 EE130 pKa = 4.12 RR131 pKa = 11.84 EE132 pKa = 3.84 QRR134 pKa = 11.84 CFDD137 pKa = 3.58 ILTGTVV143 pKa = 2.92
Molecular weight: 16.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.706
IPC_protein 10.452
Toseland 10.35
ProMoST 10.131
Dawson 10.54
Bjellqvist 10.306
Wikipedia 10.76
Rodwell 10.73
Grimsley 10.613
Solomon 10.599
Lehninger 10.57
Nozaki 10.423
DTASelect 10.277
Thurlkill 10.409
EMBOSS 10.76
Sillero 10.482
Patrickios 10.394
IPC_peptide 10.599
IPC2_peptide 9.619
IPC2.peptide.svr19 8.396
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3155
0
3155
1141124
50
1938
361.7
40.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.453 ± 0.044
1.283 ± 0.016
5.502 ± 0.029
6.577 ± 0.037
4.509 ± 0.025
6.94 ± 0.038
1.87 ± 0.017
6.78 ± 0.037
6.461 ± 0.038
9.168 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.763 ± 0.017
5.022 ± 0.033
3.746 ± 0.022
3.409 ± 0.023
4.81 ± 0.034
5.935 ± 0.035
5.635 ± 0.027
6.521 ± 0.036
1.224 ± 0.016
4.387 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here