Erwinia gerundensis
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4160 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U5KYR2|A0A0U5KYR2_9GAMM Homoserine kinase OS=Erwinia gerundensis OX=1619313 GN=EM595_0930 PE=4 SV=1
MM1 pKa = 7.73 AYY3 pKa = 10.03 EE4 pKa = 4.21 EE5 pKa = 4.33 LLEE8 pKa = 4.18 EE9 pKa = 4.15 QRR11 pKa = 11.84 EE12 pKa = 4.19 EE13 pKa = 3.64 TRR15 pKa = 11.84 LIIEE19 pKa = 4.51 EE20 pKa = 4.39 LLDD23 pKa = 4.81 DD24 pKa = 4.95 GSDD27 pKa = 3.54 PEE29 pKa = 4.03 ALYY32 pKa = 9.94 TIEE35 pKa = 5.27 HH36 pKa = 6.65 HH37 pKa = 6.81 LSCNNFDD44 pKa = 3.91 SLEE47 pKa = 4.1 KK48 pKa = 10.56 VAVDD52 pKa = 3.67 AFKK55 pKa = 10.92 LGYY58 pKa = 9.35 EE59 pKa = 3.98 VSEE62 pKa = 4.26 PEE64 pKa = 4.1 EE65 pKa = 5.11 LEE67 pKa = 4.92 LEE69 pKa = 4.66 DD70 pKa = 4.53 GSKK73 pKa = 10.88 VMCVDD78 pKa = 3.51 ILSEE82 pKa = 4.18 GALNADD88 pKa = 4.77 LIDD91 pKa = 3.82 VQVEE95 pKa = 4.04 QLVNLAGKK103 pKa = 10.59 YY104 pKa = 9.78 NVDD107 pKa = 3.2 YY108 pKa = 10.8 DD109 pKa = 3.33 GWGTYY114 pKa = 10.12 FEE116 pKa = 5.91 DD117 pKa = 5.15 PDD119 pKa = 5.85 AEE121 pKa = 4.96 DD122 pKa = 3.86 EE123 pKa = 4.45 DD124 pKa = 5.19 EE125 pKa = 4.7 EE126 pKa = 6.92 GDD128 pKa = 5.04 MIDD131 pKa = 5.97 DD132 pKa = 5.06 DD133 pKa = 4.3 DD134 pKa = 3.98 TGIRR138 pKa = 11.84 HH139 pKa = 6.18
Molecular weight: 15.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.794
IPC2_protein 3.605
IPC_protein 3.592
Toseland 3.389
ProMoST 3.745
Dawson 3.579
Bjellqvist 3.732
Wikipedia 3.49
Rodwell 3.427
Grimsley 3.3
Solomon 3.567
Lehninger 3.516
Nozaki 3.694
DTASelect 3.872
Thurlkill 3.439
EMBOSS 3.503
Sillero 3.719
Patrickios 1.786
IPC_peptide 3.567
IPC2_peptide 3.694
IPC2.peptide.svr19 3.685
Protein with the highest isoelectric point:
>tr|A0A0U5LBJ9|A0A0U5LBJ9_9GAMM Uncharacterized protein OS=Erwinia gerundensis OX=1619313 GN=EM595_p0386 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.16 GRR39 pKa = 11.84 SRR41 pKa = 11.84 LTVSKK46 pKa = 11.03
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4160
0
4160
1290596
30
3438
310.2
34.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.506 ± 0.047
0.998 ± 0.014
5.212 ± 0.029
5.39 ± 0.038
3.771 ± 0.032
7.378 ± 0.043
2.326 ± 0.019
5.572 ± 0.033
3.75 ± 0.034
11.126 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.731 ± 0.02
3.583 ± 0.028
4.559 ± 0.029
4.881 ± 0.037
5.905 ± 0.034
6.008 ± 0.032
5.269 ± 0.028
6.893 ± 0.033
1.544 ± 0.018
2.598 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here