Equine arteritis virus (EAV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Arnidovirineae; Arteriviridae; Equarterivirinae; Alphaarterivirus; Alphaarterivirus equid

Average proteome isoelectric point is 7.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q77S93|Q77S93_EAV Nucleocapsid protein OS=Equine arteritis virus OX=11047 GN=N PE=3 SV=1
MM1 pKa = 7.93LSMIVLLSLLWGAPSHH17 pKa = 6.92AYY19 pKa = 9.84FSYY22 pKa = 9.49YY23 pKa = 8.13TAQRR27 pKa = 11.84FTDD30 pKa = 4.11FTLCMLTDD38 pKa = 3.67RR39 pKa = 11.84GVIANLLRR47 pKa = 11.84YY48 pKa = 9.57DD49 pKa = 3.76EE50 pKa = 4.59HH51 pKa = 6.15TALYY55 pKa = 9.8NCSASKK61 pKa = 8.89TCWYY65 pKa = 8.61CTFLDD70 pKa = 3.81EE71 pKa = 5.32QIITFGTDD79 pKa = 2.85CNDD82 pKa = 3.21TYY84 pKa = 11.41AVPVAEE90 pKa = 4.74VLEE93 pKa = 4.4QAHH96 pKa = 6.21GLYY99 pKa = 10.49SVLFDD104 pKa = 4.99DD105 pKa = 5.08MPPFIYY111 pKa = 10.25YY112 pKa = 10.11GRR114 pKa = 11.84EE115 pKa = 3.52FGIVVLDD122 pKa = 3.16VFMFYY127 pKa = 10.43PVLVLFFLSVLPYY140 pKa = 9.36ATLILEE146 pKa = 4.33MCVSILFIIYY156 pKa = 9.84GIYY159 pKa = 10.04SGAYY163 pKa = 7.89LAMGIFAATLAIHH176 pKa = 6.54SIVVLRR182 pKa = 11.84QLLWLCLAWRR192 pKa = 11.84YY193 pKa = 10.1RR194 pKa = 11.84CTLHH198 pKa = 7.36ASFISAEE205 pKa = 4.12GKK207 pKa = 10.25VYY209 pKa = 10.23PVDD212 pKa = 4.17PGLPVAAAGNRR223 pKa = 11.84LLVPGRR229 pKa = 11.84PTIDD233 pKa = 3.26YY234 pKa = 10.46AVAYY238 pKa = 8.79GSKK241 pKa = 10.62VNLVRR246 pKa = 11.84LGAAEE251 pKa = 3.88VWEE254 pKa = 4.33PP255 pKa = 3.68

Molecular weight: 28.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9YIT7|Q9YIT7_EAV M OS=Equine arteritis virus OX=11047 GN=M PE=4 SV=1
MM1 pKa = 7.69ASRR4 pKa = 11.84RR5 pKa = 11.84SRR7 pKa = 11.84PQAASFRR14 pKa = 11.84NGRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84QPTSYY24 pKa = 11.32NDD26 pKa = 3.83LLRR29 pKa = 11.84MFGQMRR35 pKa = 11.84VRR37 pKa = 11.84KK38 pKa = 8.9PPAQPTQAIIAEE50 pKa = 4.55PGDD53 pKa = 3.77LRR55 pKa = 11.84HH56 pKa = 7.07DD57 pKa = 4.45LNQQEE62 pKa = 4.38RR63 pKa = 11.84ATLSSNVQRR72 pKa = 11.84FFMIGHH78 pKa = 7.04GSLTADD84 pKa = 3.69AGGLTYY90 pKa = 10.09TVSWVPTKK98 pKa = 10.52QIQRR102 pKa = 11.84KK103 pKa = 6.34VAPPAGPP110 pKa = 3.29

Molecular weight: 12.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

6038

67

3175

670.9

73.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.556 ± 0.244

3.362 ± 0.272

4.588 ± 0.519

3.213 ± 0.445

4.472 ± 0.668

7.486 ± 0.524

2.252 ± 0.393

3.76 ± 0.564

3.511 ± 0.675

11.047 ± 0.818

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.888 ± 0.297

2.799 ± 0.176

5.879 ± 0.37

2.965 ± 0.28

5.383 ± 0.365

7.204 ± 0.495

6.26 ± 0.245

9.026 ± 0.455

1.656 ± 0.148

3.693 ± 0.45
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski