Colinus virginianus (Northern bobwhite) (Tetrao virginianus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17122 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A226P5E4|A0A226P5E4_COLVI Uncharacterized protein OS=Colinus virginianus OX=9014 GN=H355_003667 PE=4 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84DD3 pKa = 2.77SSGIDD8 pKa = 2.96RR9 pKa = 11.84NSYY12 pKa = 10.6SGLPSNVYY20 pKa = 9.66VCSGPDD26 pKa = 3.04SALGNDD32 pKa = 4.13CAVKK36 pKa = 9.82QAEE39 pKa = 4.64TIFQEE44 pKa = 4.09MFPTEE49 pKa = 4.62EE50 pKa = 4.23FCPAPPNPEE59 pKa = 4.36DD60 pKa = 3.54IIYY63 pKa = 10.78DD64 pKa = 3.59EE65 pKa = 5.54DD66 pKa = 4.29EE67 pKa = 4.24IASEE71 pKa = 3.97EE72 pKa = 4.3TGFNNSPEE80 pKa = 4.4AKK82 pKa = 9.7PEE84 pKa = 3.97SSPQEE89 pKa = 3.91SSSRR93 pKa = 3.56

Molecular weight: 10.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A226M9S1|A0A226M9S1_COLVI Uncharacterized protein OS=Colinus virginianus OX=9014 GN=H355_001567 PE=4 SV=1
PP1 pKa = 7.03PVRR4 pKa = 11.84RR5 pKa = 11.84GAGGRR10 pKa = 11.84PAVVHH15 pKa = 6.02GAQRR19 pKa = 11.84RR20 pKa = 11.84CGRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84GVGVGVGAEE35 pKa = 3.67RR36 pKa = 11.84GGAARR41 pKa = 11.84GAGGGGARR49 pKa = 11.84GARR52 pKa = 11.84SAPRR56 pKa = 11.84AVHH59 pKa = 6.15GGGGGAAPRR68 pKa = 11.84GRR70 pKa = 11.84GVRR73 pKa = 11.84RR74 pKa = 11.84PPRR77 pKa = 11.84ARR79 pKa = 11.84EE80 pKa = 3.53PVPRR84 pKa = 11.84GRR86 pKa = 11.84RR87 pKa = 11.84HH88 pKa = 5.94AARR91 pKa = 11.84PHH93 pKa = 4.69QGARR97 pKa = 11.84VRR99 pKa = 11.84RR100 pKa = 11.84GPGGRR105 pKa = 11.84RR106 pKa = 11.84GGQPRR111 pKa = 11.84VVHH114 pKa = 6.18DD115 pKa = 3.1QHH117 pKa = 6.62PRR119 pKa = 11.84GGRR122 pKa = 11.84GRR124 pKa = 11.84SGADD128 pKa = 2.72GGGAPRR134 pKa = 11.84GGARR138 pKa = 11.84VPRR141 pKa = 11.84QRR143 pKa = 11.84RR144 pKa = 11.84RR145 pKa = 11.84VVLGALRR152 pKa = 11.84AARR155 pKa = 11.84ARR157 pKa = 11.84RR158 pKa = 11.84LRR160 pKa = 11.84RR161 pKa = 11.84EE162 pKa = 3.95HH163 pKa = 6.82PVRR166 pKa = 11.84GPPRR170 pKa = 11.84PGVPVPRR177 pKa = 11.84RR178 pKa = 11.84HH179 pKa = 5.51PTPLRR184 pKa = 11.84RR185 pKa = 11.84QQSPRR190 pKa = 11.84RR191 pKa = 11.84RR192 pKa = 11.84PRR194 pKa = 11.84AARR197 pKa = 11.84RR198 pKa = 11.84PRR200 pKa = 11.84GGTRR204 pKa = 11.84DD205 pKa = 3.57LRR207 pKa = 11.84GGLPRR212 pKa = 11.84RR213 pKa = 11.84GRR215 pKa = 11.84RR216 pKa = 11.84GAQHH220 pKa = 7.25RR221 pKa = 11.84DD222 pKa = 3.05PPRR225 pKa = 11.84ARR227 pKa = 11.84GHH229 pKa = 6.35PRR231 pKa = 11.84GAHH234 pKa = 4.81PRR236 pKa = 11.84QRR238 pKa = 11.84RR239 pKa = 11.84RR240 pKa = 11.84HH241 pKa = 5.38LRR243 pKa = 11.84HH244 pKa = 6.29LLHH247 pKa = 6.35PRR249 pKa = 11.84PRR251 pKa = 11.84RR252 pKa = 11.84GVHH255 pKa = 4.55RR256 pKa = 11.84HH257 pKa = 3.42RR258 pKa = 11.84HH259 pKa = 4.41LRR261 pKa = 11.84RR262 pKa = 11.84GPRR265 pKa = 11.84AHH267 pKa = 7.4LPPP270 pKa = 5.42

Molecular weight: 29.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17122

0

17122

8269645

9

20297

483.0

53.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.063 ± 0.02

2.245 ± 0.013

5.007 ± 0.014

7.333 ± 0.026

3.714 ± 0.013

6.212 ± 0.022

2.485 ± 0.008

4.59 ± 0.014

5.998 ± 0.025

9.729 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.246 ± 0.008

3.82 ± 0.011

5.532 ± 0.023

4.633 ± 0.016

5.507 ± 0.015

8.256 ± 0.028

5.243 ± 0.015

6.366 ± 0.015

1.167 ± 0.007

2.825 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski