Amino acid dipepetide frequency for Rhodococcus phage Trina

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.284AlaAla: 8.284 ± 1.257
0.512AlaCys: 0.512 ± 0.126
4.702AlaAsp: 4.702 ± 0.335
5.263AlaGlu: 5.263 ± 0.394
2.899AlaPhe: 2.899 ± 0.308
6.262AlaGly: 6.262 ± 0.639
0.828AlaHis: 0.828 ± 0.121
4.775AlaIle: 4.775 ± 0.31
4.824AlaLys: 4.824 ± 0.333
6.578AlaLeu: 6.578 ± 0.502
2.022AlaMet: 2.022 ± 0.294
4.069AlaAsn: 4.069 ± 0.388
3.216AlaPro: 3.216 ± 0.381
3.435AlaGln: 3.435 ± 0.744
4.556AlaArg: 4.556 ± 0.425
4.873AlaSer: 4.873 ± 0.459
5.092AlaThr: 5.092 ± 0.479
5.36AlaVal: 5.36 ± 0.406
1.511AlaTrp: 1.511 ± 0.172
2.778AlaTyr: 2.778 ± 0.281
0.0AlaXaa: 0.0 ± 0.0
Cys
0.536CysAla: 0.536 ± 0.146
0.171CysCys: 0.171 ± 0.062
0.585CysAsp: 0.585 ± 0.144
0.682CysGlu: 0.682 ± 0.136
0.439CysPhe: 0.439 ± 0.105
0.682CysGly: 0.682 ± 0.13
0.219CysHis: 0.219 ± 0.067
0.56CysIle: 0.56 ± 0.113
0.512CysLys: 0.512 ± 0.133
0.585CysLeu: 0.585 ± 0.136
0.219CysMet: 0.219 ± 0.083
0.292CysAsn: 0.292 ± 0.093
0.39CysPro: 0.39 ± 0.094
0.244CysGln: 0.244 ± 0.07
0.609CysArg: 0.609 ± 0.128
0.56CysSer: 0.56 ± 0.117
0.512CysThr: 0.512 ± 0.115
0.463CysVal: 0.463 ± 0.135
0.073CysTrp: 0.073 ± 0.04
0.39CysTyr: 0.39 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
5.36AspAla: 5.36 ± 0.385
0.804AspCys: 0.804 ± 0.146
3.606AspAsp: 3.606 ± 0.309
4.654AspGlu: 4.654 ± 0.394
3.046AspPhe: 3.046 ± 0.29
4.727AspGly: 4.727 ± 0.342
1.121AspHis: 1.121 ± 0.159
4.239AspIle: 4.239 ± 0.273
4.142AspLys: 4.142 ± 0.304
5.945AspLeu: 5.945 ± 0.363
1.827AspMet: 1.827 ± 0.231
3.411AspAsn: 3.411 ± 0.299
2.242AspPro: 2.242 ± 0.23
1.852AspGln: 1.852 ± 0.211
3.241AspArg: 3.241 ± 0.272
3.484AspSer: 3.484 ± 0.261
3.435AspThr: 3.435 ± 0.277
3.825AspVal: 3.825 ± 0.266
1.632AspTrp: 1.632 ± 0.213
2.851AspTyr: 2.851 ± 0.295
0.0AspXaa: 0.0 ± 0.0
Glu
5.36GluAla: 5.36 ± 0.453
0.439GluCys: 0.439 ± 0.103
3.85GluAsp: 3.85 ± 0.325
4.581GluGlu: 4.581 ± 0.415
3.411GluPhe: 3.411 ± 0.284
3.046GluGly: 3.046 ± 0.251
1.072GluHis: 1.072 ± 0.162
4.751GluIle: 4.751 ± 0.34
3.557GluLys: 3.557 ± 0.33
5.896GluLeu: 5.896 ± 0.494
1.73GluMet: 1.73 ± 0.217
2.875GluAsn: 2.875 ± 0.272
2.12GluPro: 2.12 ± 0.266
3.387GluGln: 3.387 ± 0.343
3.289GluArg: 3.289 ± 0.324
3.996GluSer: 3.996 ± 0.349
3.338GluThr: 3.338 ± 0.323
3.923GluVal: 3.923 ± 0.368
1.145GluTrp: 1.145 ± 0.165
2.924GluTyr: 2.924 ± 0.317
0.0GluXaa: 0.0 ± 0.0
Phe
2.802PheAla: 2.802 ± 0.217
0.463PheCys: 0.463 ± 0.1
3.265PheAsp: 3.265 ± 0.334
2.412PheGlu: 2.412 ± 0.276
1.364PhePhe: 1.364 ± 0.232
2.875PheGly: 2.875 ± 0.239
0.585PheHis: 0.585 ± 0.116
2.534PheIle: 2.534 ± 0.259
2.826PheLys: 2.826 ± 0.256
2.315PheLeu: 2.315 ± 0.305
1.145PheMet: 1.145 ± 0.172
2.266PheAsn: 2.266 ± 0.22
1.048PhePro: 1.048 ± 0.169
1.389PheGln: 1.389 ± 0.149
1.584PheArg: 1.584 ± 0.226
2.631PheSer: 2.631 ± 0.224
2.972PheThr: 2.972 ± 0.304
2.266PheVal: 2.266 ± 0.262
0.78PheTrp: 0.78 ± 0.159
1.608PheTyr: 1.608 ± 0.179
0.0PheXaa: 0.0 ± 0.0
Gly
4.946GlyAla: 4.946 ± 0.434
0.512GlyCys: 0.512 ± 0.115
3.801GlyAsp: 3.801 ± 0.315
3.241GlyGlu: 3.241 ± 0.31
3.265GlyPhe: 3.265 ± 0.262
4.386GlyGly: 4.386 ± 0.485
1.291GlyHis: 1.291 ± 0.195
4.386GlyIle: 4.386 ± 0.37
4.215GlyLys: 4.215 ± 0.311
4.8GlyLeu: 4.8 ± 0.359
1.9GlyMet: 1.9 ± 0.227
3.703GlyAsn: 3.703 ± 0.356
2.217GlyPro: 2.217 ± 0.277
2.242GlyGln: 2.242 ± 0.227
3.557GlyArg: 3.557 ± 0.313
4.775GlySer: 4.775 ± 0.382
5.336GlyThr: 5.336 ± 0.492
4.483GlyVal: 4.483 ± 0.296
1.779GlyTrp: 1.779 ± 0.235
3.192GlyTyr: 3.192 ± 0.301
0.0GlyXaa: 0.0 ± 0.0
His
1.364HisAla: 1.364 ± 0.195
0.171HisCys: 0.171 ± 0.069
1.17HisAsp: 1.17 ± 0.146
1.096HisGlu: 1.096 ± 0.185
0.755HisPhe: 0.755 ± 0.137
1.486HisGly: 1.486 ± 0.196
0.244HisHis: 0.244 ± 0.082
1.096HisIle: 1.096 ± 0.165
1.34HisLys: 1.34 ± 0.187
1.121HisLeu: 1.121 ± 0.184
0.487HisMet: 0.487 ± 0.112
0.975HisAsn: 0.975 ± 0.173
0.95HisPro: 0.95 ± 0.169
0.439HisGln: 0.439 ± 0.105
1.218HisArg: 1.218 ± 0.21
1.218HisSer: 1.218 ± 0.177
1.023HisThr: 1.023 ± 0.168
0.999HisVal: 0.999 ± 0.184
0.171HisTrp: 0.171 ± 0.058
0.999HisTyr: 0.999 ± 0.178
0.0HisXaa: 0.0 ± 0.0
Ile
5.506IleAla: 5.506 ± 0.348
0.56IleCys: 0.56 ± 0.119
4.288IleAsp: 4.288 ± 0.324
4.946IleGlu: 4.946 ± 0.377
1.974IlePhe: 1.974 ± 0.246
4.191IleGly: 4.191 ± 0.386
1.096IleHis: 1.096 ± 0.137
3.509IleIle: 3.509 ± 0.348
3.947IleLys: 3.947 ± 0.305
3.655IleLeu: 3.655 ± 0.341
1.316IleMet: 1.316 ± 0.171
3.703IleAsn: 3.703 ± 0.313
2.12IlePro: 2.12 ± 0.208
2.12IleGln: 2.12 ± 0.233
3.07IleArg: 3.07 ± 0.284
3.582IleSer: 3.582 ± 0.3
3.996IleThr: 3.996 ± 0.331
4.507IleVal: 4.507 ± 0.309
0.926IleTrp: 0.926 ± 0.161
2.144IleTyr: 2.144 ± 0.245
0.0IleXaa: 0.0 ± 0.0
Lys
5.36LysAla: 5.36 ± 0.377
0.439LysCys: 0.439 ± 0.12
3.703LysAsp: 3.703 ± 0.328
3.703LysGlu: 3.703 ± 0.345
2.266LysPhe: 2.266 ± 0.24
3.411LysGly: 3.411 ± 0.342
1.413LysHis: 1.413 ± 0.183
3.63LysIle: 3.63 ± 0.263
4.118LysLys: 4.118 ± 0.391
5.872LysLeu: 5.872 ± 0.411
1.852LysMet: 1.852 ± 0.24
3.119LysAsn: 3.119 ± 0.279
2.217LysPro: 2.217 ± 0.295
2.583LysGln: 2.583 ± 0.249
3.07LysArg: 3.07 ± 0.279
3.947LysSer: 3.947 ± 0.301
3.241LysThr: 3.241 ± 0.304
4.093LysVal: 4.093 ± 0.318
0.853LysTrp: 0.853 ± 0.143
2.729LysTyr: 2.729 ± 0.258
0.0LysXaa: 0.0 ± 0.0
Leu
6.7LeuAla: 6.7 ± 0.544
0.755LeuCys: 0.755 ± 0.149
6.262LeuAsp: 6.262 ± 0.441
5.019LeuGlu: 5.019 ± 0.408
2.851LeuPhe: 2.851 ± 0.273
5.043LeuGly: 5.043 ± 0.369
1.438LeuHis: 1.438 ± 0.213
4.97LeuIle: 4.97 ± 0.43
4.873LeuLys: 4.873 ± 0.41
5.165LeuLeu: 5.165 ± 0.448
1.974LeuMet: 1.974 ± 0.25
4.313LeuAsn: 4.313 ± 0.343
3.265LeuPro: 3.265 ± 0.298
2.461LeuGln: 2.461 ± 0.292
3.338LeuArg: 3.338 ± 0.278
5.385LeuSer: 5.385 ± 0.375
4.8LeuThr: 4.8 ± 0.334
4.678LeuVal: 4.678 ± 0.418
1.145LeuTrp: 1.145 ± 0.203
2.729LeuTyr: 2.729 ± 0.298
0.0LeuXaa: 0.0 ± 0.0
Met
1.925MetAla: 1.925 ± 0.244
0.244MetCys: 0.244 ± 0.072
0.95MetAsp: 0.95 ± 0.18
1.632MetGlu: 1.632 ± 0.218
1.048MetPhe: 1.048 ± 0.18
1.17MetGly: 1.17 ± 0.184
0.512MetHis: 0.512 ± 0.109
1.998MetIle: 1.998 ± 0.227
1.681MetLys: 1.681 ± 0.25
2.242MetLeu: 2.242 ± 0.241
0.633MetMet: 0.633 ± 0.141
0.999MetAsn: 0.999 ± 0.169
1.267MetPro: 1.267 ± 0.169
1.34MetGln: 1.34 ± 0.189
1.511MetArg: 1.511 ± 0.181
2.363MetSer: 2.363 ± 0.24
2.047MetThr: 2.047 ± 0.263
1.632MetVal: 1.632 ± 0.196
0.195MetTrp: 0.195 ± 0.073
0.926MetTyr: 0.926 ± 0.139
0.0MetXaa: 0.0 ± 0.0
Asn
4.093AsnAla: 4.093 ± 0.484
0.341AsnCys: 0.341 ± 0.099
3.509AsnAsp: 3.509 ± 0.327
3.094AsnGlu: 3.094 ± 0.269
1.559AsnPhe: 1.559 ± 0.24
4.629AsnGly: 4.629 ± 0.457
0.877AsnHis: 0.877 ± 0.159
2.851AsnIle: 2.851 ± 0.28
3.387AsnLys: 3.387 ± 0.341
4.239AsnLeu: 4.239 ± 0.382
1.194AsnMet: 1.194 ± 0.16
2.485AsnAsn: 2.485 ± 0.315
2.047AsnPro: 2.047 ± 0.195
1.852AsnGln: 1.852 ± 0.271
2.412AsnArg: 2.412 ± 0.237
3.046AsnSer: 3.046 ± 0.291
3.265AsnThr: 3.265 ± 0.387
3.509AsnVal: 3.509 ± 0.304
1.121AsnTrp: 1.121 ± 0.218
1.9AsnTyr: 1.9 ± 0.181
0.0AsnXaa: 0.0 ± 0.0
Pro
3.046ProAla: 3.046 ± 0.387
0.268ProCys: 0.268 ± 0.094
2.875ProAsp: 2.875 ± 0.282
3.094ProGlu: 3.094 ± 0.335
1.267ProPhe: 1.267 ± 0.18
2.972ProGly: 2.972 ± 0.274
0.731ProHis: 0.731 ± 0.137
1.925ProIle: 1.925 ± 0.26
2.51ProLys: 2.51 ± 0.315
2.193ProLeu: 2.193 ± 0.23
0.95ProMet: 0.95 ± 0.16
1.925ProAsn: 1.925 ± 0.226
1.096ProPro: 1.096 ± 0.163
1.072ProGln: 1.072 ± 0.17
1.754ProArg: 1.754 ± 0.246
2.071ProSer: 2.071 ± 0.255
2.631ProThr: 2.631 ± 0.257
3.289ProVal: 3.289 ± 0.394
0.56ProTrp: 0.56 ± 0.12
1.121ProTyr: 1.121 ± 0.183
0.0ProXaa: 0.0 ± 0.0
Gln
3.509GlnAla: 3.509 ± 0.391
0.244GlnCys: 0.244 ± 0.077
1.974GlnAsp: 1.974 ± 0.25
2.217GlnGlu: 2.217 ± 0.226
1.413GlnPhe: 1.413 ± 0.186
2.315GlnGly: 2.315 ± 0.21
0.658GlnHis: 0.658 ± 0.128
2.363GlnIle: 2.363 ± 0.224
2.266GlnLys: 2.266 ± 0.273
3.362GlnLeu: 3.362 ± 0.367
0.853GlnMet: 0.853 ± 0.123
2.071GlnAsn: 2.071 ± 0.306
1.194GlnPro: 1.194 ± 0.179
1.803GlnGln: 1.803 ± 0.318
2.193GlnArg: 2.193 ± 0.351
2.022GlnSer: 2.022 ± 0.279
2.534GlnThr: 2.534 ± 0.29
2.339GlnVal: 2.339 ± 0.242
0.901GlnTrp: 0.901 ± 0.133
1.316GlnTyr: 1.316 ± 0.185
0.0GlnXaa: 0.0 ± 0.0
Arg
3.411ArgAla: 3.411 ± 0.335
0.487ArgCys: 0.487 ± 0.102
3.338ArgAsp: 3.338 ± 0.338
3.63ArgGlu: 3.63 ± 0.323
2.022ArgPhe: 2.022 ± 0.255
2.924ArgGly: 2.924 ± 0.253
1.145ArgHis: 1.145 ± 0.156
3.289ArgIle: 3.289 ± 0.306
3.192ArgLys: 3.192 ± 0.274
4.264ArgLeu: 4.264 ± 0.266
1.657ArgMet: 1.657 ± 0.175
2.485ArgAsn: 2.485 ± 0.202
1.632ArgPro: 1.632 ± 0.188
2.193ArgGln: 2.193 ± 0.269
2.412ArgArg: 2.412 ± 0.32
2.924ArgSer: 2.924 ± 0.294
2.631ArgThr: 2.631 ± 0.226
3.387ArgVal: 3.387 ± 0.339
0.975ArgTrp: 0.975 ± 0.151
2.168ArgTyr: 2.168 ± 0.221
0.0ArgXaa: 0.0 ± 0.0
Ser
5.214SerAla: 5.214 ± 0.555
0.512SerCys: 0.512 ± 0.116
4.775SerAsp: 4.775 ± 0.332
3.825SerGlu: 3.825 ± 0.298
2.388SerPhe: 2.388 ± 0.253
4.995SerGly: 4.995 ± 0.449
1.34SerHis: 1.34 ± 0.171
3.314SerIle: 3.314 ± 0.293
3.411SerLys: 3.411 ± 0.314
4.751SerLeu: 4.751 ± 0.365
1.949SerMet: 1.949 ± 0.268
3.192SerAsn: 3.192 ± 0.299
2.217SerPro: 2.217 ± 0.256
2.144SerGln: 2.144 ± 0.221
3.411SerArg: 3.411 ± 0.306
3.898SerSer: 3.898 ± 0.497
4.459SerThr: 4.459 ± 0.352
4.702SerVal: 4.702 ± 0.347
0.901SerTrp: 0.901 ± 0.134
2.68SerTyr: 2.68 ± 0.261
0.0SerXaa: 0.0 ± 0.0
Thr
5.019ThrAla: 5.019 ± 0.516
0.439ThrCys: 0.439 ± 0.112
3.971ThrAsp: 3.971 ± 0.307
3.777ThrGlu: 3.777 ± 0.338
3.021ThrPhe: 3.021 ± 0.264
4.995ThrGly: 4.995 ± 0.428
1.121ThrHis: 1.121 ± 0.172
3.898ThrIle: 3.898 ± 0.302
3.533ThrLys: 3.533 ± 0.311
4.751ThrLeu: 4.751 ± 0.315
1.511ThrMet: 1.511 ± 0.195
2.899ThrAsn: 2.899 ± 0.405
2.948ThrPro: 2.948 ± 0.303
2.461ThrGln: 2.461 ± 0.294
2.729ThrArg: 2.729 ± 0.231
4.288ThrSer: 4.288 ± 0.372
3.509ThrThr: 3.509 ± 0.421
4.873ThrVal: 4.873 ± 0.365
0.999ThrTrp: 0.999 ± 0.167
2.047ThrTyr: 2.047 ± 0.289
0.0ThrXaa: 0.0 ± 0.0
Val
5.238ValAla: 5.238 ± 0.5
0.731ValCys: 0.731 ± 0.146
4.581ValAsp: 4.581 ± 0.411
4.361ValGlu: 4.361 ± 0.345
2.144ValPhe: 2.144 ± 0.256
4.045ValGly: 4.045 ± 0.301
1.364ValHis: 1.364 ± 0.203
3.898ValIle: 3.898 ± 0.328
4.191ValLys: 4.191 ± 0.323
5.141ValLeu: 5.141 ± 0.423
1.925ValMet: 1.925 ± 0.174
3.265ValAsn: 3.265 ± 0.324
2.851ValPro: 2.851 ± 0.305
2.339ValGln: 2.339 ± 0.241
3.801ValArg: 3.801 ± 0.359
4.702ValSer: 4.702 ± 0.335
4.337ValThr: 4.337 ± 0.376
5.092ValVal: 5.092 ± 0.41
0.901ValTrp: 0.901 ± 0.17
2.388ValTyr: 2.388 ± 0.233
0.0ValXaa: 0.0 ± 0.0
Trp
1.218TrpAla: 1.218 ± 0.175
0.171TrpCys: 0.171 ± 0.061
1.218TrpAsp: 1.218 ± 0.166
1.145TrpGlu: 1.145 ± 0.196
0.633TrpPhe: 0.633 ± 0.104
1.291TrpGly: 1.291 ± 0.169
0.585TrpHis: 0.585 ± 0.135
1.17TrpIle: 1.17 ± 0.194
1.096TrpLys: 1.096 ± 0.165
1.34TrpLeu: 1.34 ± 0.189
0.39TrpMet: 0.39 ± 0.095
0.804TrpAsn: 0.804 ± 0.173
0.804TrpPro: 0.804 ± 0.148
0.707TrpGln: 0.707 ± 0.119
0.707TrpArg: 0.707 ± 0.128
0.901TrpSer: 0.901 ± 0.153
1.267TrpThr: 1.267 ± 0.196
1.145TrpVal: 1.145 ± 0.177
0.268TrpTrp: 0.268 ± 0.089
0.682TrpTyr: 0.682 ± 0.124
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.948TyrAla: 2.948 ± 0.282
0.463TyrCys: 0.463 ± 0.113
3.021TyrAsp: 3.021 ± 0.306
2.315TyrGlu: 2.315 ± 0.304
1.364TyrPhe: 1.364 ± 0.181
2.583TyrGly: 2.583 ± 0.23
0.682TyrHis: 0.682 ± 0.124
1.998TyrIle: 1.998 ± 0.224
1.998TyrLys: 1.998 ± 0.216
2.948TyrLeu: 2.948 ± 0.271
0.755TyrMet: 0.755 ± 0.165
2.558TyrAsn: 2.558 ± 0.341
1.511TyrPro: 1.511 ± 0.238
1.486TyrGln: 1.486 ± 0.235
1.706TyrArg: 1.706 ± 0.228
3.411TyrSer: 3.411 ± 0.31
2.363TyrThr: 2.363 ± 0.273
2.729TyrVal: 2.729 ± 0.299
0.707TyrTrp: 0.707 ± 0.145
1.852TyrTyr: 1.852 ± 0.242
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 250 proteins (41044 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski