Escherichia phage vB_EcoM_IME392

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385ISM7|A0A385ISM7_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoM_IME392 OX=2301649 PE=4 SV=1
MM1 pKa = 7.26NKK3 pKa = 10.22AEE5 pKa = 4.22VVLGLVQSVDD15 pKa = 3.21EE16 pKa = 5.48GYY18 pKa = 11.11SSVKK22 pKa = 10.11FCPDD26 pKa = 2.49SDD28 pKa = 3.28AWIDD32 pKa = 3.55NLKK35 pKa = 10.37QVISEE40 pKa = 4.06VADD43 pKa = 3.59YY44 pKa = 11.25DD45 pKa = 4.12KK46 pKa = 11.79LNFDD50 pKa = 4.13TGHH53 pKa = 7.26DD54 pKa = 3.68NHH56 pKa = 6.49CTLMYY61 pKa = 10.72APGTGAPDD69 pKa = 3.43EE70 pKa = 4.27GTIADD75 pKa = 4.01LVGSKK80 pKa = 10.04LHH82 pKa = 6.29LKK84 pKa = 9.47NPRR87 pKa = 11.84YY88 pKa = 10.0EE89 pKa = 4.12IFGEE93 pKa = 4.0NANYY97 pKa = 10.2LVLAFDD103 pKa = 4.24SDD105 pKa = 4.19LLQSFNYY112 pKa = 9.73RR113 pKa = 11.84LRR115 pKa = 11.84ASGEE119 pKa = 4.15VPSEE123 pKa = 3.68ISAEE127 pKa = 3.99YY128 pKa = 10.17SPHH131 pKa = 5.23ITLAKK136 pKa = 10.3DD137 pKa = 3.44VEE139 pKa = 4.27NLDD142 pKa = 3.79VSTLPEE148 pKa = 4.89LPTDD152 pKa = 4.25LYY154 pKa = 11.79LNITGVTAEE163 pKa = 4.66EE164 pKa = 4.43IKK166 pKa = 11.04NDD168 pKa = 3.46

Molecular weight: 18.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385ISR4|A0A385ISR4_9CAUD Putative tail protein OS=Escherichia phage vB_EcoM_IME392 OX=2301649 PE=4 SV=1
MM1 pKa = 8.3DD2 pKa = 3.93IRR4 pKa = 11.84SLVLQGTDD12 pKa = 3.14TLRR15 pKa = 11.84KK16 pKa = 8.6NQYY19 pKa = 7.38PTGKK23 pKa = 10.28VGSFDD28 pKa = 3.58FNYY31 pKa = 10.4VMRR34 pKa = 11.84NKK36 pKa = 10.06QLIVKK41 pKa = 8.88SQARR45 pKa = 11.84PSSGEE50 pKa = 3.99SYY52 pKa = 10.65SAYY55 pKa = 9.63QSQIVFSGVGYY66 pKa = 10.7SDD68 pKa = 3.82VPSPDD73 pKa = 3.26FPLTYY78 pKa = 10.26NSPNGKK84 pKa = 9.37IYY86 pKa = 10.23LQKK89 pKa = 9.06PTTRR93 pKa = 11.84NTVQIRR99 pKa = 11.84CSCKK103 pKa = 9.57DD104 pKa = 3.88DD105 pKa = 3.75YY106 pKa = 11.91FMWQYY111 pKa = 10.89PNKK114 pKa = 10.53SKK116 pKa = 10.91GALLGRR122 pKa = 11.84FKK124 pKa = 10.83PYY126 pKa = 9.93VRR128 pKa = 11.84KK129 pKa = 7.2TTDD132 pKa = 3.04RR133 pKa = 11.84PPRR136 pKa = 11.84NPGNVPGMCKK146 pKa = 9.79HH147 pKa = 6.36ALALVKK153 pKa = 10.49NLFSMGVLQPDD164 pKa = 3.89SQVQEE169 pKa = 4.16YY170 pKa = 10.38LSRR173 pKa = 11.84PTRR176 pKa = 11.84SII178 pKa = 3.21

Molecular weight: 20.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

29279

136

1095

344.5

38.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.896 ± 0.238

1.018 ± 0.088

6.769 ± 0.165

6.257 ± 0.25

3.815 ± 0.143

7.152 ± 0.232

1.861 ± 0.104

6.36 ± 0.159

5.796 ± 0.198

8.354 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.801 ± 0.118

5.12 ± 0.155

4.006 ± 0.157

3.265 ± 0.122

4.665 ± 0.164

7.026 ± 0.151

6.264 ± 0.267

7.107 ± 0.22

1.434 ± 0.089

4.034 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski