Menghai rhabdovirus
Average proteome isoelectric point is 7.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L2YVG6|A0A1L2YVG6_9RHAB GDP polyribonucleotidyltransferase OS=Menghai rhabdovirus OX=1919071 PE=4 SV=1
MM1 pKa = 7.98 DD2 pKa = 3.66 AHH4 pKa = 7.27 KK5 pKa = 10.23 FRR7 pKa = 11.84 YY8 pKa = 9.46 VDD10 pKa = 3.51 GSGKK14 pKa = 10.39 AWVKK18 pKa = 10.38 DD19 pKa = 3.71 VPSINMGDD27 pKa = 3.82 SVDD30 pKa = 6.33 DD31 pKa = 3.63 PTTWLNDD38 pKa = 3.32 GKK40 pKa = 10.52 RR41 pKa = 11.84 PKK43 pKa = 9.83 LTIPKK48 pKa = 9.82 AKK50 pKa = 10.58 SNMLKK55 pKa = 9.18 TVCKK59 pKa = 10.38 YY60 pKa = 9.74 FASQSASPEE69 pKa = 4.04 NIEE72 pKa = 4.42 PDD74 pKa = 3.3 VALYY78 pKa = 10.72 LLYY81 pKa = 10.37 HH82 pKa = 6.63 LSVKK86 pKa = 9.94 YY87 pKa = 10.6 IKK89 pKa = 10.57 VDD91 pKa = 3.16 IKK93 pKa = 11.5 NKK95 pKa = 8.78 LTLKK99 pKa = 10.72 KK100 pKa = 10.39 NIYY103 pKa = 9.81 LNSGLKK109 pKa = 9.24 TVDD112 pKa = 3.71 DD113 pKa = 4.57 LFDD116 pKa = 3.72 VEE118 pKa = 4.92 EE119 pKa = 5.12 GEE121 pKa = 4.22 EE122 pKa = 4.14 LKK124 pKa = 11.22 LSGDD128 pKa = 3.68 NEE130 pKa = 4.29 TSDD133 pKa = 4.49 RR134 pKa = 11.84 EE135 pKa = 4.18 MPEE138 pKa = 4.49 LEE140 pKa = 4.29 ILSALLANYY149 pKa = 7.63 RR150 pKa = 11.84 ICGMASNADD159 pKa = 3.41 QKK161 pKa = 11.37 YY162 pKa = 8.49 VQTLKK167 pKa = 11.12 ARR169 pKa = 11.84 MNRR172 pKa = 11.84 TFVQKK177 pKa = 10.34 QIIKK181 pKa = 10.71 EE182 pKa = 4.02 EE183 pKa = 4.0 IQEE186 pKa = 4.06 NFYY189 pKa = 11.37 NALTWVNNYY198 pKa = 10.55 NYY200 pKa = 9.5 TRR202 pKa = 11.84 LCAGIDD208 pKa = 3.47 LLLFSDD214 pKa = 4.15 KK215 pKa = 10.65 NHH217 pKa = 7.16 PYY219 pKa = 10.09 SALRR223 pKa = 11.84 MATQSSKK230 pKa = 10.9 FKK232 pKa = 10.58 DD233 pKa = 3.42 CALIGEE239 pKa = 4.62 INHH242 pKa = 6.59 FLTTLSEE249 pKa = 4.35 APMDD253 pKa = 3.69 AWEE256 pKa = 4.52 HH257 pKa = 5.93 ALEE260 pKa = 3.98 QSISKK265 pKa = 9.97 EE266 pKa = 3.69 IINIMTCLGRR276 pKa = 11.84 GFEE279 pKa = 4.38 GEE281 pKa = 4.08 YY282 pKa = 11.02 ANYY285 pKa = 9.26 AKK287 pKa = 10.74 EE288 pKa = 3.94 MGAMDD293 pKa = 4.64 KK294 pKa = 11.28 SPFSVTANPNLHH306 pKa = 5.86 NWMHH310 pKa = 6.84 MICAMLSSEE319 pKa = 3.86 RR320 pKa = 11.84 SMNARR325 pKa = 11.84 LASGNQHH332 pKa = 5.21 TVFLTALACAYY343 pKa = 8.1 TYY345 pKa = 10.61 RR346 pKa = 11.84 SKK348 pKa = 10.07 TKK350 pKa = 9.58 IQAVIYY356 pKa = 10.49 KK357 pKa = 9.74 DD358 pKa = 4.08 DD359 pKa = 4.39 HH360 pKa = 7.07 DD361 pKa = 3.97 SHH363 pKa = 7.23 ARR365 pKa = 11.84 SINEE369 pKa = 3.99 STDD372 pKa = 3.26 DD373 pKa = 3.72 QIGMLEE379 pKa = 3.94 TAEE382 pKa = 4.01 NLKK385 pKa = 10.59 ALRR388 pKa = 11.84 DD389 pKa = 4.06 DD390 pKa = 4.19 GEE392 pKa = 4.21 EE393 pKa = 4.14 ASEE396 pKa = 3.49 IWEE399 pKa = 3.94 WFNNKK404 pKa = 7.93 KK405 pKa = 9.2 TIISDD410 pKa = 3.61 EE411 pKa = 4.26 RR412 pKa = 11.84 PGTIGSYY419 pKa = 10.4 LISKK423 pKa = 7.51 MHH425 pKa = 6.34
Molecular weight: 48.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.5
IPC2_protein 5.499
IPC_protein 5.512
Toseland 5.804
ProMoST 5.715
Dawson 5.69
Bjellqvist 5.741
Wikipedia 5.652
Rodwell 5.652
Grimsley 5.906
Solomon 5.69
Lehninger 5.664
Nozaki 5.919
DTASelect 6.097
Thurlkill 6.033
EMBOSS 5.982
Sillero 6.008
Patrickios 4.062
IPC_peptide 5.703
IPC2_peptide 5.995
IPC2.peptide.svr19 6.054
Protein with the highest isoelectric point:
>tr|A0A1L2YVG9|A0A1L2YVG9_9RHAB G protein OS=Menghai rhabdovirus OX=1919071 PE=4 SV=1
MM1 pKa = 7.35 TKK3 pKa = 10.4 NIRR6 pKa = 11.84 TILSDD11 pKa = 4.08 FILRR15 pKa = 11.84 QKK17 pKa = 9.38 IAKK20 pKa = 10.02 DD21 pKa = 2.74 IGEE24 pKa = 4.4 DD25 pKa = 3.13 KK26 pKa = 11.05 KK27 pKa = 11.08 GFYY30 pKa = 10.15 YY31 pKa = 10.73 SRR33 pKa = 11.84 IDD35 pKa = 3.72 GPIMNN40 pKa = 5.24
Molecular weight: 4.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.904
IPC2_protein 9.004
IPC_protein 9.048
Toseland 9.78
ProMoST 9.414
Dawson 9.97
Bjellqvist 9.604
Wikipedia 10.116
Rodwell 10.423
Grimsley 10.028
Solomon 10.043
Lehninger 10.028
Nozaki 9.721
DTASelect 9.604
Thurlkill 9.809
EMBOSS 10.16
Sillero 9.867
Patrickios 10.292
IPC_peptide 10.043
IPC2_peptide 8.083
IPC2.peptide.svr19 7.973
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3364
40
2032
560.7
64.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.805 ± 0.88
1.486 ± 0.368
6.361 ± 0.389
6.897 ± 0.633
4.102 ± 0.395
4.251 ± 0.238
2.14 ± 0.217
8.383 ± 0.678
9.721 ± 1.343
9.245 ± 0.947
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.032 ± 0.367
7.134 ± 0.191
3.151 ± 0.103
2.497 ± 0.302
3.686 ± 0.531
8.205 ± 0.764
4.637 ± 0.491
4.786 ± 0.388
1.249 ± 0.119
5.232 ± 0.482
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here