Gordonia phage Samba

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514TZX0|A0A514TZX0_9CAUD Uncharacterized protein OS=Gordonia phage Samba OX=2593336 GN=34 PE=4 SV=1
MM1 pKa = 7.46ANDD4 pKa = 4.61TIFEE8 pKa = 4.28LPEE11 pKa = 4.65IPGVTFTASYY21 pKa = 10.84GSGGEE26 pKa = 4.12TGLPSNWIRR35 pKa = 11.84IVGTIEE41 pKa = 3.97NPWYY45 pKa = 10.1EE46 pKa = 3.66PTYY49 pKa = 11.14NYY51 pKa = 11.12GLDD54 pKa = 4.0PNGHH58 pKa = 5.86TEE60 pKa = 4.02MTDD63 pKa = 2.47PWKK66 pKa = 10.72RR67 pKa = 11.84HH68 pKa = 3.79TQFPEE73 pKa = 3.86VCAMGFGGPSIGLPTDD89 pKa = 3.88PPPPLVEE96 pKa = 4.64PEE98 pKa = 4.07PTPDD102 pKa = 3.89IIEE105 pKa = 4.47EE106 pKa = 4.28PTDD109 pKa = 3.47GG110 pKa = 4.67

Molecular weight: 11.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514TZY4|A0A514TZY4_9CAUD Tail assembly chaperone OS=Gordonia phage Samba OX=2593336 GN=16 PE=4 SV=1
MM1 pKa = 6.42TTNVPIHH8 pKa = 6.57RR9 pKa = 11.84EE10 pKa = 3.47EE11 pKa = 3.84VARR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 4.31AIAEE21 pKa = 4.11LTRR24 pKa = 11.84AGLSAPEE31 pKa = 3.48IAIRR35 pKa = 11.84LNITARR41 pKa = 11.84TVVRR45 pKa = 11.84HH46 pKa = 5.74RR47 pKa = 11.84ALAGVSKK54 pKa = 8.77PTHH57 pKa = 5.77PRR59 pKa = 11.84LTEE62 pKa = 3.86EE63 pKa = 3.94QLATARR69 pKa = 11.84RR70 pKa = 11.84LIDD73 pKa = 3.23EE74 pKa = 4.53GAPYY78 pKa = 10.44KK79 pKa = 10.61EE80 pKa = 3.81IARR83 pKa = 11.84TIGCHH88 pKa = 5.41EE89 pKa = 4.35STVAQHH95 pKa = 6.05FPGRR99 pKa = 11.84GWTRR103 pKa = 11.84KK104 pKa = 10.05QIDD107 pKa = 3.05EE108 pKa = 3.86WRR110 pKa = 11.84RR111 pKa = 11.84EE112 pKa = 3.61VRR114 pKa = 11.84LWVDD118 pKa = 3.44ASS120 pKa = 3.44

Molecular weight: 13.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

16763

52

1830

212.2

23.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.502 ± 0.444

0.907 ± 0.145

7.057 ± 0.331

5.655 ± 0.257

2.667 ± 0.157

8.34 ± 0.39

2.136 ± 0.168

4.617 ± 0.143

3.353 ± 0.218

7.696 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.106 ± 0.168

2.959 ± 0.131

5.793 ± 0.245

3.406 ± 0.142

7.457 ± 0.436

5.244 ± 0.212

6.986 ± 0.247

7.916 ± 0.198

2.004 ± 0.124

2.201 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski