Prochlorococcus marinus (strain SARG / CCMP1375 / SS120)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Prochloraceae; Prochlorococcus; Prochlorococcus marinus; Prochlorococcus marinus subsp. marinus

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1881 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q7VCK5|UPPP_PROMA Undecaprenyl-diphosphatase OS=Prochlorococcus marinus (strain SARG / CCMP1375 / SS120) OX=167539 GN=uppP PE=3 SV=1
MM1 pKa = 7.62KK2 pKa = 10.48LFRR5 pKa = 11.84QLLVAPAALGLLAPLAANAAEE26 pKa = 4.18VNIKK30 pKa = 10.36DD31 pKa = 3.69VASYY35 pKa = 11.62ADD37 pKa = 4.12LNAEE41 pKa = 3.98VTTTQFSDD49 pKa = 3.75VVPGDD54 pKa = 3.31WAYY57 pKa = 8.91TALQNLSEE65 pKa = 4.8SYY67 pKa = 10.92GCVDD71 pKa = 3.74NAYY74 pKa = 7.76TQNLKK79 pKa = 10.5SGQALTRR86 pKa = 11.84YY87 pKa = 7.92EE88 pKa = 3.79AAALVNACLDD98 pKa = 3.53GGIASAEE105 pKa = 4.19VTPDD109 pKa = 3.07AARR112 pKa = 11.84LANEE116 pKa = 5.14FGTEE120 pKa = 3.7MAILKK125 pKa = 10.21GRR127 pKa = 11.84VDD129 pKa = 3.35GLEE132 pKa = 4.03YY133 pKa = 10.33KK134 pKa = 10.72VKK136 pKa = 10.1EE137 pKa = 3.98LSAGQFSSSTKK148 pKa = 10.17LSGSAVFTTGAIDD161 pKa = 4.06GMSLFDD167 pKa = 5.4DD168 pKa = 4.92PYY170 pKa = 10.25KK171 pKa = 11.09GKK173 pKa = 8.0TAFEE177 pKa = 3.87YY178 pKa = 10.43RR179 pKa = 11.84YY180 pKa = 10.54GIDD183 pKa = 4.41LNTSFNGTDD192 pKa = 3.26GLYY195 pKa = 10.74AGIEE199 pKa = 3.97QGNQDD204 pKa = 4.93DD205 pKa = 5.85LVLQDD210 pKa = 4.78AVTDD214 pKa = 3.66STLTVTSLFYY224 pKa = 10.8NFQVGEE230 pKa = 4.17FDD232 pKa = 3.76VTAGPLFDD240 pKa = 3.78QDD242 pKa = 4.63DD243 pKa = 4.72VISATTSIASEE254 pKa = 4.24SFRR257 pKa = 11.84LSSMPWGAVATTGAGAAIAYY277 pKa = 10.59SNDD280 pKa = 3.12SGFNASLSSVSSDD293 pKa = 3.62AADD296 pKa = 3.34ASKK299 pKa = 11.1GIWTDD304 pKa = 3.21GGADD308 pKa = 3.43IYY310 pKa = 10.28TASLGYY316 pKa = 10.79DD317 pKa = 2.88SDD319 pKa = 4.88YY320 pKa = 11.5YY321 pKa = 11.33GGGLIYY327 pKa = 10.55TDD329 pKa = 5.37DD330 pKa = 4.15DD331 pKa = 4.04TDD333 pKa = 3.38TSFGGGIYY341 pKa = 8.92MRR343 pKa = 11.84PDD345 pKa = 4.02GFPTISVAYY354 pKa = 7.22DD355 pKa = 3.5TKK357 pKa = 11.36DD358 pKa = 3.13PAASGTKK365 pKa = 9.94SSSHH369 pKa = 6.21LLIGVDD375 pKa = 3.69HH376 pKa = 6.65EE377 pKa = 4.81VGPGTASAAYY387 pKa = 8.22TNHH390 pKa = 6.63DD391 pKa = 3.57QMGVTGDD398 pKa = 3.43SFEE401 pKa = 4.22IYY403 pKa = 10.46YY404 pKa = 10.18NYY406 pKa = 9.66PVSDD410 pKa = 4.01GLSLQAGVFWEE421 pKa = 4.31DD422 pKa = 2.71RR423 pKa = 11.84HH424 pKa = 5.29VVKK427 pKa = 10.89NGSWTTQDD435 pKa = 2.7ATGYY439 pKa = 10.11LVEE442 pKa = 4.76TNFSFF447 pKa = 4.81

Molecular weight: 47.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7VB24|Q7VB24_PROMA Uncharacterized protein OS=Prochlorococcus marinus (strain SARG / CCMP1375 / SS120) OX=167539 GN=Pro_1276 PE=4 SV=1
MM1 pKa = 7.41TKK3 pKa = 9.05RR4 pKa = 11.84TFGGTSRR11 pKa = 11.84KK12 pKa = 9.13RR13 pKa = 11.84KK14 pKa = 8.17RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84THH26 pKa = 6.87TGRR29 pKa = 11.84SVIRR33 pKa = 11.84SRR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.33KK38 pKa = 8.85GRR40 pKa = 11.84SRR42 pKa = 11.84IAVV45 pKa = 3.34

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1881

0

1881

516670

30

1524

274.7

30.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.904 ± 0.06

1.221 ± 0.02

4.94 ± 0.04

6.433 ± 0.051

4.068 ± 0.039

6.879 ± 0.057

1.815 ± 0.031

7.818 ± 0.052

6.603 ± 0.057

11.523 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.949 ± 0.02

4.976 ± 0.044

4.252 ± 0.03

3.666 ± 0.031

4.93 ± 0.046

7.719 ± 0.045

4.695 ± 0.042

5.702 ± 0.05

1.461 ± 0.03

2.446 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski