Candidatus Evansia muelleri

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Zymobacter group; Candidatus Evansia

Average proteome isoelectric point is 8.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 330 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A078KEQ5|A0A078KEQ5_9GAMM Ketol-acid reductoisomerase (NADP(+)) OS=Candidatus Evansia muelleri OX=1495769 GN=ilvC PE=3 SV=1
MM1 pKa = 7.36AFVVTEE7 pKa = 3.75NCILCKK13 pKa = 9.94YY14 pKa = 4.8TTCVEE19 pKa = 4.23VCPVDD24 pKa = 4.13CFYY27 pKa = 11.12EE28 pKa = 4.9GPNFLVINPDD38 pKa = 3.11EE39 pKa = 5.59CIDD42 pKa = 3.87CALCEE47 pKa = 4.26PEE49 pKa = 5.15CPAKK53 pKa = 10.51AIYY56 pKa = 9.92SEE58 pKa = 4.62NEE60 pKa = 3.23IPQNQKK66 pKa = 10.61KK67 pKa = 10.01FIEE70 pKa = 4.49LNSEE74 pKa = 4.79LSYY77 pKa = 10.63IWPNINKK84 pKa = 9.98KK85 pKa = 10.0KK86 pKa = 10.45DD87 pKa = 3.31QLKK90 pKa = 10.36YY91 pKa = 9.8AEE93 pKa = 4.53KK94 pKa = 9.95WDD96 pKa = 4.64GILGKK101 pKa = 10.19IKK103 pKa = 10.31FLEE106 pKa = 4.35KK107 pKa = 10.69

Molecular weight: 12.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A078KE31|A0A078KE31_9GAMM Ribulose-phosphate 3-epimerase OS=Candidatus Evansia muelleri OX=1495769 GN=rpe PE=3 SV=1
MM1 pKa = 7.12FGEE4 pKa = 4.38YY5 pKa = 9.3IIKK8 pKa = 9.9IRR10 pKa = 11.84ASIKK14 pKa = 10.27RR15 pKa = 11.84MCRR18 pKa = 11.84KK19 pKa = 9.57CKK21 pKa = 9.82IISRR25 pKa = 11.84YY26 pKa = 9.8GILRR30 pKa = 11.84VICTDD35 pKa = 3.46PKK37 pKa = 10.25HH38 pKa = 6.27KK39 pKa = 10.12QRR41 pKa = 11.84QGG43 pKa = 2.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

330

0

330

111405

34

1368

337.6

38.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.91 ± 0.11

1.389 ± 0.043

4.105 ± 0.077

4.787 ± 0.102

4.661 ± 0.091

5.973 ± 0.109

1.907 ± 0.045

14.033 ± 0.169

9.124 ± 0.149

9.265 ± 0.104

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.061 ± 0.051

8.438 ± 0.138

3.059 ± 0.06

2.413 ± 0.049

3.732 ± 0.083

5.794 ± 0.064

4.292 ± 0.063

4.264 ± 0.08

0.873 ± 0.042

4.92 ± 0.087
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski