Altererythrobacter xiamenensis
Average proteome isoelectric point is 5.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2951 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y6EKA2|A0A1Y6EKA2_9SPHN PAP2 superfamily protein OS=Altererythrobacter xiamenensis OX=1316679 GN=SAMN06297468_0444 PE=4 SV=1
MM1 pKa = 7.07 VPARR5 pKa = 11.84 LTEE8 pKa = 4.17 TLNMNTPKK16 pKa = 9.86 IDD18 pKa = 3.69 LASLPGLDD26 pKa = 3.3 TVTGIFGSTAQAGAAYY42 pKa = 9.93 DD43 pKa = 4.05 DD44 pKa = 4.1 GVVAIMVYY52 pKa = 10.41 VYY54 pKa = 11.07 DD55 pKa = 4.07 VVPPSAIVV63 pKa = 2.98
Molecular weight: 6.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.872
IPC_protein 3.681
Toseland 3.49
ProMoST 3.935
Dawson 3.732
Bjellqvist 3.91
Wikipedia 3.757
Rodwell 3.541
Grimsley 3.427
Solomon 3.668
Lehninger 3.63
Nozaki 3.897
DTASelect 4.126
Thurlkill 3.617
EMBOSS 3.757
Sillero 3.834
Patrickios 1.914
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|A0A1Y6F3N6|A0A1Y6F3N6_9SPHN Cupin domain protein OS=Altererythrobacter xiamenensis OX=1316679 GN=SAMN06297468_1681 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.89 GFFARR21 pKa = 11.84 KK22 pKa = 8.1 ATTGGRR28 pKa = 11.84 KK29 pKa = 8.12 VLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 8.68 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.82 KK41 pKa = 10.45 LCAA44 pKa = 3.96
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.425
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.369
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.087
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2951
0
2951
930223
28
2332
315.2
34.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.993 ± 0.059
0.799 ± 0.014
6.247 ± 0.041
6.92 ± 0.048
3.745 ± 0.03
8.734 ± 0.049
1.916 ± 0.023
5.223 ± 0.028
3.415 ± 0.036
9.555 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.573 ± 0.025
2.774 ± 0.031
4.997 ± 0.026
3.186 ± 0.021
6.69 ± 0.039
5.528 ± 0.033
5.166 ± 0.035
6.85 ± 0.039
1.432 ± 0.019
2.257 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here