Acanthocystis turfacea chlorella virus 1

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Chlorovirus

Average proteome isoelectric point is 7.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 860 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7K9U2|A7K9U2_9PHYC Uncharacterized protein z682R OS=Acanthocystis turfacea chlorella virus 1 OX=322019 GN=z682R PE=4 SV=1
MM1 pKa = 7.97PLQKK5 pKa = 10.21VIRR8 pKa = 11.84IIRR11 pKa = 11.84EE12 pKa = 3.47NWVFIAIVVVVVFALAMMMRR32 pKa = 11.84KK33 pKa = 8.55EE34 pKa = 4.42RR35 pKa = 11.84YY36 pKa = 8.39MEE38 pKa = 4.59GCSLCTPGDD47 pKa = 3.61AGVLPGSVFTMSQIMNLDD65 pKa = 3.71KK66 pKa = 10.96EE67 pKa = 4.72LEE69 pKa = 4.24GCKK72 pKa = 10.22LCSLGDD78 pKa = 3.67AGVLPGSVFTMKK90 pKa = 10.51QIMDD94 pKa = 4.39LDD96 pKa = 4.21KK97 pKa = 10.79EE98 pKa = 4.33TDD100 pKa = 3.67DD101 pKa = 5.01FDD103 pKa = 4.88EE104 pKa = 4.68EE105 pKa = 4.36LAGCSTCDD113 pKa = 3.75AGDD116 pKa = 3.62AGILLDD122 pKa = 4.39TEE124 pKa = 4.64TTPEE128 pKa = 3.78TEE130 pKa = 4.45MYY132 pKa = 11.1NMPDD136 pKa = 3.23EE137 pKa = 4.88YY138 pKa = 11.02YY139 pKa = 10.95GSDD142 pKa = 3.26VKK144 pKa = 11.29VADD147 pKa = 4.85TIAEE151 pKa = 4.17VAANPSEE158 pKa = 3.87IAAVVEE164 pKa = 4.31DD165 pKa = 4.53RR166 pKa = 11.84EE167 pKa = 4.2EE168 pKa = 4.93DD169 pKa = 3.79DD170 pKa = 3.97EE171 pKa = 4.39PMYY174 pKa = 7.46MTPLEE179 pKa = 4.1YY180 pKa = 10.56QNTVEE185 pKa = 4.96QEE187 pKa = 4.01LDD189 pKa = 3.56YY190 pKa = 10.35MSPMQFQDD198 pKa = 3.19NLAQAIYY205 pKa = 10.25NATVDD210 pKa = 3.88KK211 pKa = 10.59PYY213 pKa = 11.01VDD215 pKa = 5.44NEE217 pKa = 4.17DD218 pKa = 3.91ADD220 pKa = 4.33LMSIDD225 pKa = 4.97PIDD228 pKa = 3.6YY229 pKa = 8.87TLQSDD234 pKa = 3.88AQLVNDD240 pKa = 5.25FYY242 pKa = 11.83GDD244 pKa = 3.88DD245 pKa = 3.95EE246 pKa = 4.97IDD248 pKa = 3.44YY249 pKa = 10.92KK250 pKa = 10.88EE251 pKa = 4.36RR252 pKa = 11.84EE253 pKa = 3.93RR254 pKa = 11.84IAAVKK259 pKa = 10.09ALPIDD264 pKa = 4.79FYY266 pKa = 11.14MSDD269 pKa = 3.07SSFTLLL275 pKa = 4.43

Molecular weight: 30.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7K916|A7K916_9PHYC Uncharacterized protein z406R OS=Acanthocystis turfacea chlorella virus 1 OX=322019 GN=z406R PE=4 SV=1
MM1 pKa = 7.61LLLLPLQTFSHH12 pKa = 6.87RR13 pKa = 11.84KK14 pKa = 7.98PLQPLPPRR22 pKa = 11.84TFSHH26 pKa = 6.56RR27 pKa = 11.84HH28 pKa = 5.02PLLPLLLTRR37 pKa = 11.84FRR39 pKa = 11.84KK40 pKa = 7.75TRR42 pKa = 11.84QANLQQAFFRR52 pKa = 11.84SPRR55 pKa = 11.84PRR57 pKa = 11.84PVSRR61 pKa = 11.84RR62 pKa = 11.84SLSQRR67 pKa = 11.84HH68 pKa = 5.28HH69 pKa = 6.5RR70 pKa = 4.01

Molecular weight: 8.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

860

0

860

145517

65

1450

169.2

18.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.379 ± 0.105

2.145 ± 0.07

4.738 ± 0.1

4.665 ± 0.12

4.753 ± 0.097

6.436 ± 0.179

2.464 ± 0.087

5.379 ± 0.086

5.624 ± 0.157

8.334 ± 0.143

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.91 ± 0.072

4.26 ± 0.136

5.378 ± 0.137

2.954 ± 0.084

6.287 ± 0.146

8.077 ± 0.156

6.361 ± 0.113

7.523 ± 0.126

1.277 ± 0.048

3.055 ± 0.065
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski