Ralstonia phage Simangalove

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Bakolyvirus; Ralstonia virus Simangalove

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5BBR4|A0A7G5BBR4_9CAUD Uncharacterized protein OS=Ralstonia phage Simangalove OX=2759710 GN=R1_00035 PE=4 SV=1
MM1 pKa = 7.82DD2 pKa = 4.71TLLLDD7 pKa = 3.81TSTWDD12 pKa = 3.36LTTDD16 pKa = 3.19ASGNLATVGDD26 pKa = 4.47ADD28 pKa = 4.71RR29 pKa = 11.84DD30 pKa = 3.98ATNGGALRR38 pKa = 11.84LAQDD42 pKa = 3.25VAARR46 pKa = 11.84VSSWRR51 pKa = 11.84GEE53 pKa = 4.1VYY55 pKa = 10.89YY56 pKa = 9.65DD57 pKa = 3.35TTQGIPYY64 pKa = 8.49DD65 pKa = 4.38TILGEE70 pKa = 4.18YY71 pKa = 8.8PNLVVLQQLYY81 pKa = 10.57SEE83 pKa = 4.84EE84 pKa = 4.2SLNVPGCATALAEE97 pKa = 4.16LTYY100 pKa = 11.24DD101 pKa = 3.22RR102 pKa = 11.84AGRR105 pKa = 11.84LLGGTINVSDD115 pKa = 3.62ISGNPAVVTLL125 pKa = 4.35

Molecular weight: 13.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5BBS8|A0A7G5BBS8_9CAUD Uncharacterized protein OS=Ralstonia phage Simangalove OX=2759710 GN=R1_00049 PE=4 SV=1
MM1 pKa = 7.31SVFVLGRR8 pKa = 11.84NGAPLMPCSEE18 pKa = 3.67QRR20 pKa = 11.84ARR22 pKa = 11.84KK23 pKa = 9.03LLSAGRR29 pKa = 11.84ARR31 pKa = 11.84VHH33 pKa = 6.32RR34 pKa = 11.84LYY36 pKa = 10.32PFTIRR41 pKa = 11.84LIDD44 pKa = 3.61RR45 pKa = 11.84QQANSVLQPLEE56 pKa = 4.54LKK58 pKa = 10.42LDD60 pKa = 4.35PGSKK64 pKa = 6.87TTGLAVCRR72 pKa = 11.84VEE74 pKa = 4.85EE75 pKa = 4.68RR76 pKa = 11.84IDD78 pKa = 3.29VDD80 pKa = 3.97GVVEE84 pKa = 4.1RR85 pKa = 11.84VMHH88 pKa = 6.39IRR90 pKa = 11.84FLMDD94 pKa = 3.92LVHH97 pKa = 6.97RR98 pKa = 11.84GHH100 pKa = 7.53AIKK103 pKa = 10.34EE104 pKa = 4.13ALHH107 pKa = 6.32ARR109 pKa = 11.84AAMRR113 pKa = 11.84RR114 pKa = 11.84RR115 pKa = 11.84RR116 pKa = 11.84RR117 pKa = 11.84GNLRR121 pKa = 11.84YY122 pKa = 9.46RR123 pKa = 11.84APRR126 pKa = 11.84FDD128 pKa = 2.98NRR130 pKa = 11.84TRR132 pKa = 11.84KK133 pKa = 9.91EE134 pKa = 3.62GWLPPSLQHH143 pKa = 7.23RR144 pKa = 11.84VDD146 pKa = 3.81TTVSWVKK153 pKa = 10.15RR154 pKa = 11.84LCRR157 pKa = 11.84LAPITHH163 pKa = 7.53LAQEE167 pKa = 4.45LVRR170 pKa = 11.84FDD172 pKa = 3.56MQLMQNAEE180 pKa = 3.87ISGVEE185 pKa = 4.02YY186 pKa = 10.62QQGEE190 pKa = 4.04LAGYY194 pKa = 7.23EE195 pKa = 3.88VRR197 pKa = 11.84EE198 pKa = 3.92YY199 pKa = 11.21LLEE202 pKa = 4.35KK203 pKa = 10.41FGRR206 pKa = 11.84TCQYY210 pKa = 10.63CDD212 pKa = 3.25EE213 pKa = 4.8TGVPLQVEE221 pKa = 4.92HH222 pKa = 6.68IHH224 pKa = 6.08PKK226 pKa = 10.4ARR228 pKa = 11.84GGSNRR233 pKa = 11.84VSNLTLACQPCNEE246 pKa = 4.9KK247 pKa = 10.41KK248 pKa = 10.31DD249 pKa = 3.8SQDD252 pKa = 2.61IRR254 pKa = 11.84VFLAKK259 pKa = 10.57DD260 pKa = 3.18PMRR263 pKa = 11.84LEE265 pKa = 5.13RR266 pKa = 11.84ILARR270 pKa = 11.84AKK272 pKa = 10.84APLRR276 pKa = 11.84DD277 pKa = 3.54AAAVNATRR285 pKa = 11.84WALLNALKK293 pKa = 9.42GTGLPVTTGSGGQTKK308 pKa = 9.43WNRR311 pKa = 11.84TRR313 pKa = 11.84LGLPKK318 pKa = 9.65THH320 pKa = 6.85SLDD323 pKa = 3.5AACVGRR329 pKa = 11.84ADD331 pKa = 3.88RR332 pKa = 11.84VTGSGAPVLVVKK344 pKa = 9.21CTGRR348 pKa = 11.84GSRR351 pKa = 11.84SRR353 pKa = 11.84TRR355 pKa = 11.84LNQYY359 pKa = 8.61GFPRR363 pKa = 11.84AYY365 pKa = 10.63LARR368 pKa = 11.84NKK370 pKa = 9.22TAFGFRR376 pKa = 11.84TGDD379 pKa = 3.32MVIASVPSGKK389 pKa = 10.13KK390 pKa = 9.39AGTYY394 pKa = 9.16KK395 pKa = 10.63GRR397 pKa = 11.84VAIRR401 pKa = 11.84QTGSFNIQPGKK412 pKa = 9.8PGLPTVQGISHH423 pKa = 5.92KK424 pKa = 9.82HH425 pKa = 5.11CRR427 pKa = 11.84VAQRR431 pKa = 11.84GDD433 pKa = 3.13GYY435 pKa = 11.29GYY437 pKa = 11.49ALASPANSQPGKK449 pKa = 9.96AKK451 pKa = 10.4RR452 pKa = 11.84PAASALYY459 pKa = 10.63LLGLKK464 pKa = 10.53AEE466 pKa = 4.36VSLADD471 pKa = 3.42

Molecular weight: 52.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

13237

52

917

228.2

24.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.979 ± 0.428

0.997 ± 0.114

5.636 ± 0.257

5.092 ± 0.375

3.294 ± 0.202

8.038 ± 0.581

1.76 ± 0.178

4.132 ± 0.172

3.611 ± 0.284

8.393 ± 0.32

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.156

3.709 ± 0.23

5.613 ± 0.229

4.314 ± 0.189

6.285 ± 0.42

5.651 ± 0.317

6.716 ± 0.444

7.147 ± 0.279

1.518 ± 0.127

2.863 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski