Mycobacterium virus Kssjeb

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1D6X1|G1D6X1_9CAUD Minor tail protein OS=Mycobacterium virus Kssjeb OX=1034109 GN=27 PE=4 SV=1
MM1 pKa = 7.93KK2 pKa = 9.35YY3 pKa = 7.78TLRR6 pKa = 11.84VFGIPVLSFEE16 pKa = 4.46SAGTGTEE23 pKa = 3.43EE24 pKa = 5.41GYY26 pKa = 10.65INLTGGSFEE35 pKa = 4.65LAPEE39 pKa = 3.98EE40 pKa = 4.15PEE42 pKa = 3.9YY43 pKa = 11.13DD44 pKa = 3.29EE45 pKa = 5.61EE46 pKa = 4.95YY47 pKa = 10.82YY48 pKa = 10.93EE49 pKa = 4.22EE50 pKa = 4.6DD51 pKa = 3.1RR52 pKa = 11.84SGFGFGVSS60 pKa = 3.28

Molecular weight: 6.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1D6U6|G1D6U6_9CAUD Uncharacterized protein OS=Mycobacterium virus Kssjeb OX=1034109 GN=2 PE=4 SV=1
MM1 pKa = 8.11SGMSLGRR8 pKa = 11.84AMGWEE13 pKa = 3.89SSDD16 pKa = 3.21RR17 pKa = 11.84RR18 pKa = 11.84EE19 pKa = 4.33RR20 pKa = 11.84LPADD24 pKa = 3.37WPRR27 pKa = 11.84IRR29 pKa = 11.84RR30 pKa = 11.84DD31 pKa = 3.26VLRR34 pKa = 11.84AAGHH38 pKa = 6.42RR39 pKa = 11.84CQIRR43 pKa = 11.84YY44 pKa = 9.91ADD46 pKa = 3.88ICTGMATEE54 pKa = 4.27VDD56 pKa = 3.34HH57 pKa = 7.08VRR59 pKa = 11.84YY60 pKa = 9.51RR61 pKa = 11.84DD62 pKa = 3.58EE63 pKa = 4.33EE64 pKa = 4.6SPTRR68 pKa = 11.84AACKK72 pKa = 9.61ACHH75 pKa = 5.71ARR77 pKa = 11.84KK78 pKa = 9.55SAMEE82 pKa = 4.09GVAQRR87 pKa = 11.84AKK89 pKa = 10.29LRR91 pKa = 11.84AMKK94 pKa = 9.94KK95 pKa = 9.63RR96 pKa = 11.84PPPRR100 pKa = 11.84HH101 pKa = 5.87PGRR104 pKa = 11.84RR105 pKa = 11.84SNN107 pKa = 3.52

Molecular weight: 12.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

16129

24

822

175.3

19.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.149 ± 0.435

0.676 ± 0.102

6.584 ± 0.246

6.535 ± 0.297

3.199 ± 0.167

8.723 ± 0.515

2.003 ± 0.192

4.923 ± 0.219

4.073 ± 0.225

8.519 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.089 ± 0.129

3.156 ± 0.139

5.499 ± 0.212

3.255 ± 0.181

6.349 ± 0.398

5.878 ± 0.281

6.299 ± 0.285

7.155 ± 0.179

2.021 ± 0.126

2.914 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski