Staphylococcus warneri
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6JPB2|A0A1W6JPB2_STAWA Integrase OS=Staphylococcus warneri OX=1292 PE=3 SV=1
MM1 pKa = 7.81 NEE3 pKa = 3.75 EE4 pKa = 3.89 QLKK7 pKa = 10.23 VIEE10 pKa = 5.47 DD11 pKa = 3.78 IYY13 pKa = 10.7 STLGSVANDD22 pKa = 3.0 KK23 pKa = 9.76 ATEE26 pKa = 3.97 YY27 pKa = 10.66 EE28 pKa = 4.26 HH29 pKa = 6.69 YY30 pKa = 10.31 FKK32 pKa = 10.81 EE33 pKa = 4.53 GHH35 pKa = 5.82 NEE37 pKa = 3.2 WTEE40 pKa = 4.07 TVDD43 pKa = 5.3 RR44 pKa = 11.84 EE45 pKa = 4.06 QHH47 pKa = 4.94 LQAIIEE53 pKa = 4.17 WALQQIEE60 pKa = 4.59 NNFEE64 pKa = 4.31 LEE66 pKa = 4.08 NEE68 pKa = 4.12
Molecular weight: 8.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.277
IPC2_protein 4.304
IPC_protein 4.151
Toseland 4.024
ProMoST 4.19
Dawson 4.075
Bjellqvist 4.291
Wikipedia 3.923
Rodwell 3.999
Grimsley 3.935
Solomon 4.075
Lehninger 4.024
Nozaki 4.202
DTASelect 4.253
Thurlkill 4.024
EMBOSS 3.935
Sillero 4.266
Patrickios 3.3
IPC_peptide 4.088
IPC2_peptide 4.253
IPC2.peptide.svr19 4.19
Protein with the highest isoelectric point:
>tr|A0A1W6JPH5|A0A1W6JPH5_STAWA Uncharacterized protein OS=Staphylococcus warneri OX=1292 PE=4 SV=1
MM1 pKa = 6.99 SQRR4 pKa = 11.84 DD5 pKa = 3.57 KK6 pKa = 11.04 MFEE9 pKa = 3.84 KK10 pKa = 10.81 LKK12 pKa = 10.7 RR13 pKa = 11.84 NPKK16 pKa = 10.42 NIDD19 pKa = 3.04 LRR21 pKa = 11.84 EE22 pKa = 3.97 FEE24 pKa = 5.17 SILKK28 pKa = 10.46 KK29 pKa = 10.77 FGFVVDD35 pKa = 3.71 KK36 pKa = 10.92 TSGKK40 pKa = 9.67 GSHH43 pKa = 7.5 FIYY46 pKa = 10.07 YY47 pKa = 9.65 HH48 pKa = 6.23 PSKK51 pKa = 10.95 SEE53 pKa = 3.25 IRR55 pKa = 11.84 RR56 pKa = 11.84 TVNKK60 pKa = 9.3 TSPLRR65 pKa = 11.84 KK66 pKa = 8.71 HH67 pKa = 7.14 HH68 pKa = 6.48 IRR70 pKa = 11.84 TLIKK74 pKa = 10.55 DD75 pKa = 3.47 IEE77 pKa = 4.33 QVIKK81 pKa = 10.94
Molecular weight: 9.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.145
IPC2_protein 9.56
IPC_protein 9.589
Toseland 10.643
ProMoST 10.16
Dawson 10.716
Bjellqvist 10.277
Wikipedia 10.818
Rodwell 11.33
Grimsley 10.73
Solomon 10.745
Lehninger 10.745
Nozaki 10.599
DTASelect 10.292
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.613
Patrickios 11.082
IPC_peptide 10.76
IPC2_peptide 8.639
IPC2.peptide.svr19 8.667
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24
0
24
4020
39
532
167.5
19.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.93 ± 0.38
0.522 ± 0.114
6.269 ± 0.467
7.836 ± 0.391
4.677 ± 0.325
3.408 ± 0.502
2.015 ± 0.337
9.179 ± 0.576
10.075 ± 0.432
8.483 ± 0.615
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.488 ± 0.295
8.458 ± 0.42
1.915 ± 0.242
3.706 ± 0.214
3.607 ± 0.389
6.716 ± 0.408
5.05 ± 0.301
5.572 ± 0.342
0.846 ± 0.1
5.249 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here