Massilia glaciei

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5316 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U2HNZ8|A0A2U2HNZ8_9BURK Uncharacterized protein OS=Massilia glaciei OX=1524097 GN=C7C56_007525 PE=4 SV=1
MM1 pKa = 7.1NAVAEE6 pKa = 4.28MQDD9 pKa = 4.02VIPAPIVFTDD19 pKa = 3.68SAAAKK24 pKa = 8.97VAQLIEE30 pKa = 4.39EE31 pKa = 4.51EE32 pKa = 4.63GNPDD36 pKa = 2.85LKK38 pKa = 11.13LRR40 pKa = 11.84VFVQGGGCSGFQYY53 pKa = 10.94GFTFDD58 pKa = 4.69EE59 pKa = 4.93IVNEE63 pKa = 4.83DD64 pKa = 3.85DD65 pKa = 3.11TTMVKK70 pKa = 10.58NGVQLLIDD78 pKa = 3.77SMSYY82 pKa = 10.23QYY84 pKa = 11.55LVGAEE89 pKa = 4.01IDD91 pKa = 3.89YY92 pKa = 11.28KK93 pKa = 11.39DD94 pKa = 4.47DD95 pKa = 4.08LEE97 pKa = 4.56GAQFVIKK104 pKa = 10.68NPTATSTCGCGSSFSAA120 pKa = 4.79

Molecular weight: 12.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U2HHV0|A0A2U2HHV0_9BURK Chromate transporter OS=Massilia glaciei OX=1524097 GN=C7C56_017555 PE=3 SV=1
MM1 pKa = 7.32PQLPALPQLPWLPQLPALPPPPLPPPPLPRR31 pKa = 11.84LALPPPPSPQPPSLTPPSPPLSPQPARR58 pKa = 11.84PLRR61 pKa = 11.84PSPRR65 pKa = 11.84HH66 pKa = 5.41RR67 pKa = 11.84PRR69 pKa = 11.84HH70 pKa = 4.89RR71 pKa = 11.84LRR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84VRR78 pKa = 11.84PLRR81 pKa = 11.84RR82 pKa = 11.84LPSRR86 pKa = 11.84RR87 pKa = 11.84GRR89 pKa = 11.84GGCGCARR96 pKa = 11.84RR97 pKa = 11.84GGGGGAARR105 pKa = 11.84RR106 pKa = 11.84RR107 pKa = 11.84PPRR110 pKa = 11.84LRR112 pKa = 11.84SPRR115 pKa = 11.84RR116 pKa = 11.84SGRR119 pKa = 11.84SRR121 pKa = 3.15

Molecular weight: 13.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5316

0

5316

1765612

32

10199

332.1

35.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.262 ± 0.053

0.94 ± 0.013

5.309 ± 0.026

5.08 ± 0.031

3.649 ± 0.022

8.536 ± 0.051

2.184 ± 0.019

4.593 ± 0.027

3.621 ± 0.031

10.576 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.573 ± 0.018

2.955 ± 0.028

5.011 ± 0.034

3.554 ± 0.021

6.704 ± 0.049

5.447 ± 0.031

5.012 ± 0.044

7.285 ± 0.031

1.334 ± 0.016

2.377 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski