Helicobacter sp. MIT 99-5507

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter; unclassified Helicobacter

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1538 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D8HXH0|A0A3D8HXH0_9HELI Uncharacterized protein OS=Helicobacter sp. MIT 99-5507 OX=152489 GN=CQA42_06150 PE=4 SV=1
MM1 pKa = 8.06SLMINDD7 pKa = 3.48EE8 pKa = 4.84CIACDD13 pKa = 3.47ACRR16 pKa = 11.84EE17 pKa = 4.11EE18 pKa = 5.06CPNEE22 pKa = 4.7AIDD25 pKa = 4.18EE26 pKa = 4.34NDD28 pKa = 4.43PIYY31 pKa = 10.86LIDD34 pKa = 4.4PDD36 pKa = 4.17KK37 pKa = 10.95CTEE40 pKa = 3.95CVGYY44 pKa = 10.32YY45 pKa = 10.16DD46 pKa = 4.66EE47 pKa = 5.02PACISVCPVDD57 pKa = 6.06AIVADD62 pKa = 4.06PDD64 pKa = 3.68NVEE67 pKa = 5.07SIDD70 pKa = 3.53EE71 pKa = 4.22LKK73 pKa = 11.26YY74 pKa = 10.74KK75 pKa = 9.88FQKK78 pKa = 10.5LSQRR82 pKa = 11.84DD83 pKa = 3.29

Molecular weight: 9.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D8HVK2|A0A3D8HVK2_9HELI Uncharacterized protein OS=Helicobacter sp. MIT 99-5507 OX=152489 GN=CQA42_07360 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.09QPHH8 pKa = 6.8KK9 pKa = 9.91KK10 pKa = 9.44PKK12 pKa = 8.47KK13 pKa = 5.9TTHH16 pKa = 5.93GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.77TKK25 pKa = 10.06NGQRR29 pKa = 11.84VIRR32 pKa = 11.84NRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.79GRR39 pKa = 11.84KK40 pKa = 8.77RR41 pKa = 11.84LAAA44 pKa = 4.42

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1538

0

1538

462701

24

2886

300.8

34.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.74 ± 0.055

1.188 ± 0.025

5.989 ± 0.049

5.713 ± 0.065

5.5 ± 0.062

5.675 ± 0.071

1.539 ± 0.023

11.041 ± 0.074

8.921 ± 0.077

9.783 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.228 ± 0.023

7.15 ± 0.072

2.753 ± 0.032

2.771 ± 0.031

3.189 ± 0.032

7.165 ± 0.047

4.108 ± 0.041

4.851 ± 0.051

0.635 ± 0.019

4.061 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski