Caulobacter phage CcrBL9
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 517 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385EFM9|A0A385EFM9_9CAUD Uncharacterized protein OS=Caulobacter phage CcrBL9 OX=2283270 GN=CcrBL9_gp455 PE=4 SV=1
MM1 pKa = 7.6 AAFLINTDD9 pKa = 3.62 LNALVEE15 pKa = 4.87 DD16 pKa = 3.62 IAYY19 pKa = 9.97 DD20 pKa = 3.59 VEE22 pKa = 4.4 MEE24 pKa = 4.05 DD25 pKa = 4.23 RR26 pKa = 11.84 AAEE29 pKa = 4.1 EE30 pKa = 4.09 LDD32 pKa = 3.71 GMDD35 pKa = 4.79 FGDD38 pKa = 3.41 RR39 pKa = 11.84 CYY41 pKa = 11.1 YY42 pKa = 10.57 GDD44 pKa = 3.74 RR45 pKa = 11.84 AYY47 pKa = 10.55 HH48 pKa = 5.72 GQLEE52 pKa = 4.2 LARR55 pKa = 11.84 IIEE58 pKa = 4.69 AATAAHH64 pKa = 7.29 PILYY68 pKa = 10.25 AA69 pKa = 4.22
Molecular weight: 7.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.802
IPC2_protein 4.024
IPC_protein 3.935
Toseland 3.745
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.846
Rodwell 3.77
Grimsley 3.656
Solomon 3.897
Lehninger 3.859
Nozaki 4.05
DTASelect 4.228
Thurlkill 3.795
EMBOSS 3.859
Sillero 4.062
Patrickios 0.846
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.938
Protein with the highest isoelectric point:
>tr|A0A385EB25|A0A385EB25_9CAUD Uncharacterized protein OS=Caulobacter phage CcrBL9 OX=2283270 GN=CcrBL9_gp050c PE=4 SV=1
MM1 pKa = 7.86 RR2 pKa = 11.84 ARR4 pKa = 11.84 LALLLAGVTGALVLSLTRR22 pKa = 11.84 PVSARR27 pKa = 11.84 TKK29 pKa = 10.28 LISLAARR36 pKa = 11.84 PPWVGRR42 pKa = 11.84 KK43 pKa = 9.64 LSVPVV48 pKa = 3.49
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.441
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
517
0
517
88936
37
2748
172.0
18.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.924 ± 0.199
0.905 ± 0.056
6.651 ± 0.165
6.264 ± 0.221
3.668 ± 0.082
8.125 ± 0.502
2.256 ± 0.102
5.0 ± 0.097
4.531 ± 0.172
8.337 ± 0.186
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.456 ± 0.09
3.071 ± 0.124
5.069 ± 0.125
3.42 ± 0.103
6.297 ± 0.197
5.06 ± 0.154
6.07 ± 0.203
6.903 ± 0.128
1.788 ± 0.063
3.207 ± 0.091
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here