Staphylococcus phage Terranova

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Twortvirinae; Sepunavirus; unclassified Sepunavirus

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 205 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385IKK8|A0A385IKK8_9CAUD NTP pyrophosphohydrolase OS=Staphylococcus phage Terranova OX=2282407 GN=Terranova_044 PE=4 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84KK3 pKa = 9.19INPVIKK9 pKa = 10.65GEE11 pKa = 4.2SKK13 pKa = 10.43KK14 pKa = 10.85VQTTDD19 pKa = 3.04VKK21 pKa = 10.67KK22 pKa = 9.27VSYY25 pKa = 9.52YY26 pKa = 10.89VRR28 pKa = 11.84DD29 pKa = 3.85HH30 pKa = 6.48NPAMTIEE37 pKa = 4.56DD38 pKa = 3.89SEE40 pKa = 5.08DD41 pKa = 3.45YY42 pKa = 11.01NSNSQYY48 pKa = 11.04LVSDD52 pKa = 3.26NGKK55 pKa = 10.45FIAKK59 pKa = 9.55YY60 pKa = 8.56NKK62 pKa = 9.03EE63 pKa = 3.9VNAVGFYY70 pKa = 10.66EE71 pKa = 4.07EE72 pKa = 4.27SGVNIKK78 pKa = 10.41HH79 pKa = 5.78LTHH82 pKa = 6.33TTPEE86 pKa = 4.1RR87 pKa = 11.84LEE89 pKa = 4.14GTVFTIEE96 pKa = 4.17EE97 pKa = 4.2DD98 pKa = 3.96TEE100 pKa = 4.33IDD102 pKa = 4.76LINDD106 pKa = 3.58VLPQGDD112 pKa = 3.48ILIRR116 pKa = 11.84TSYY119 pKa = 11.56DD120 pKa = 3.12EE121 pKa = 5.51VLTLDD126 pKa = 3.68NEE128 pKa = 4.57SVEE131 pKa = 5.12DD132 pKa = 3.7YY133 pKa = 11.03LIYY136 pKa = 10.9ADD138 pKa = 4.83IDD140 pKa = 4.0LDD142 pKa = 3.94DD143 pKa = 4.35VEE145 pKa = 4.92EE146 pKa = 4.83IIYY149 pKa = 9.76TGLSKK154 pKa = 11.1VKK156 pKa = 9.09TEE158 pKa = 3.91NVIVDD163 pKa = 3.43VSLNKK168 pKa = 10.38YY169 pKa = 10.06INLDD173 pKa = 4.05DD174 pKa = 5.6IEE176 pKa = 6.02IEE178 pKa = 5.63DD179 pKa = 4.3DD180 pKa = 4.14TLCKK184 pKa = 10.8NCDD187 pKa = 3.89CIDD190 pKa = 3.46YY191 pKa = 8.57CTITGEE197 pKa = 4.32EE198 pKa = 3.93II199 pKa = 3.37

Molecular weight: 22.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385IKQ4|A0A385IKQ4_9CAUD Membrane protein OS=Staphylococcus phage Terranova OX=2282407 GN=Terranova_100 PE=4 SV=1
MM1 pKa = 7.43AKK3 pKa = 9.86KK4 pKa = 10.5VKK6 pKa = 8.88ATKK9 pKa = 10.41KK10 pKa = 8.94PTIFKK15 pKa = 10.32NGKK18 pKa = 6.98QVSRR22 pKa = 11.84KK23 pKa = 8.06TIDD26 pKa = 2.99SMRR29 pKa = 11.84SDD31 pKa = 4.21ILDD34 pKa = 3.78KK35 pKa = 11.24LQATAQQVDD44 pKa = 3.54DD45 pKa = 4.64VYY47 pKa = 10.79IKK49 pKa = 10.81RR50 pKa = 11.84MPTYY54 pKa = 10.96LEE56 pKa = 3.97VTEE59 pKa = 5.14KK60 pKa = 10.79KK61 pKa = 10.69LEE63 pKa = 4.12EE64 pKa = 4.5KK65 pKa = 10.88GVIDD69 pKa = 5.17LKK71 pKa = 11.16KK72 pKa = 10.8AFAKK76 pKa = 10.27SSKK79 pKa = 9.86KK80 pKa = 10.06RR81 pKa = 11.84RR82 pKa = 11.84TKK84 pKa = 10.55NGGWYY89 pKa = 10.02LIVPIRR95 pKa = 11.84IKK97 pKa = 9.34TSRR100 pKa = 11.84MNRR103 pKa = 11.84DD104 pKa = 3.05TYY106 pKa = 11.28QDD108 pKa = 3.35MRR110 pKa = 11.84KK111 pKa = 8.95LQIPSGKK118 pKa = 9.98SSASSLTSYY127 pKa = 10.37LQAVSKK133 pKa = 10.42GRR135 pKa = 11.84NITHH139 pKa = 7.51PSMKK143 pKa = 9.84PKK145 pKa = 10.61SPSNNMTKK153 pKa = 9.77VRR155 pKa = 11.84RR156 pKa = 11.84KK157 pKa = 8.29PKK159 pKa = 10.05KK160 pKa = 7.89KK161 pKa = 9.47ASYY164 pKa = 10.05FIFRR168 pKa = 11.84TVSSKK173 pKa = 10.92SPSSAWVLNRR183 pKa = 11.84DD184 pKa = 3.99KK185 pKa = 11.56VNSDD189 pKa = 3.12NFSKK193 pKa = 8.23TTLNNVKK200 pKa = 10.55NLMNWKK206 pKa = 8.26MRR208 pKa = 11.84NIRR211 pKa = 3.6

Molecular weight: 24.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

205

0

205

40432

29

1151

197.2

22.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.396 ± 0.18

0.658 ± 0.061

6.764 ± 0.125

8.325 ± 0.231

4.207 ± 0.099

5.001 ± 0.172

1.618 ± 0.089

7.927 ± 0.213

9.896 ± 0.27

8.281 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.149 ± 0.074

7.437 ± 0.165

2.57 ± 0.13

3.341 ± 0.2

3.601 ± 0.119

6.356 ± 0.159

5.951 ± 0.185

6.356 ± 0.144

0.804 ± 0.058

5.362 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski