Capybara microvirus Cap1_SP_70
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W3Z1|A0A4P8W3Z1_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_70 OX=2584789 PE=4 SV=1
MM1 pKa = 7.76 RR2 pKa = 11.84 VTKK5 pKa = 10.31 KK6 pKa = 10.49 LYY8 pKa = 10.18 KK9 pKa = 9.02 PCEE12 pKa = 3.73 YY13 pKa = 10.49 SPRR16 pKa = 11.84 SLHH19 pKa = 7.49 FITNPDD25 pKa = 3.64 NPDD28 pKa = 3.2 VLIPEE33 pKa = 4.52 CTEE36 pKa = 4.01 SDD38 pKa = 3.18 IKK40 pKa = 10.96 FYY42 pKa = 11.13 DD43 pKa = 3.58 LSLVSLQNAGIDD55 pKa = 3.65 VQKK58 pKa = 11.17 FNLKK62 pKa = 8.99 TSAPSRR68 pKa = 11.84 LEE70 pKa = 3.76 KK71 pKa = 10.67 EE72 pKa = 4.06 MSVMQMGEE80 pKa = 4.25 IISKK84 pKa = 10.39 HH85 pKa = 5.9 FDD87 pKa = 3.22 TEE89 pKa = 4.43 TLDD92 pKa = 4.61 KK93 pKa = 10.98 PIDD96 pKa = 3.84 EE97 pKa = 4.93 QKK99 pKa = 9.85 TEE101 pKa = 3.75
Molecular weight: 11.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.068
IPC2_protein 5.156
IPC_protein 5.003
Toseland 4.914
ProMoST 4.978
Dawson 4.978
Bjellqvist 5.169
Wikipedia 4.851
Rodwell 4.889
Grimsley 4.838
Solomon 4.978
Lehninger 4.94
Nozaki 5.105
DTASelect 5.245
Thurlkill 4.927
EMBOSS 4.889
Sillero 5.169
Patrickios 4.202
IPC_peptide 4.991
IPC2_peptide 5.156
IPC2.peptide.svr19 5.199
Protein with the highest isoelectric point:
>tr|A0A4P8W4Q9|A0A4P8W4Q9_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_70 OX=2584789 PE=3 SV=1
MM1 pKa = 7.59 EE2 pKa = 4.35 SAFYY6 pKa = 10.6 KK7 pKa = 10.32 IFNPKK12 pKa = 9.32 KK13 pKa = 9.36 CFDD16 pKa = 3.25 MRR18 pKa = 11.84 YY19 pKa = 10.12 DD20 pKa = 3.28 KK21 pKa = 11.13 DD22 pKa = 3.78 YY23 pKa = 11.43 FILSKK28 pKa = 10.99 SNSYY32 pKa = 11.34 VNMRR36 pKa = 11.84 QKK38 pKa = 10.32 QLSMEE43 pKa = 3.47 IRR45 pKa = 11.84 LYY47 pKa = 11.38 YY48 pKa = 9.96 EE49 pKa = 4.52 FEE51 pKa = 4.32 YY52 pKa = 9.33 CQKK55 pKa = 10.17 MNGRR59 pKa = 11.84 VYY61 pKa = 10.9 FLTFTYY67 pKa = 10.9 NNNNIPYY74 pKa = 9.59 FLGEE78 pKa = 4.13 PCPDD82 pKa = 3.38 KK83 pKa = 11.25 KK84 pKa = 11.13 DD85 pKa = 3.37 MQRR88 pKa = 11.84 FLNHH92 pKa = 5.76 SRR94 pKa = 11.84 FARR97 pKa = 11.84 NLEE100 pKa = 4.08 KK101 pKa = 10.28 IYY103 pKa = 10.42 GYY105 pKa = 10.32 KK106 pKa = 10.28 FKK108 pKa = 10.92 YY109 pKa = 10.13 FCATEE114 pKa = 4.28 LGDD117 pKa = 3.94 GKK119 pKa = 10.58 GKK121 pKa = 10.51 RR122 pKa = 11.84 GIGKK126 pKa = 8.6 NPHH129 pKa = 5.0 FHH131 pKa = 6.93 VIFFLYY137 pKa = 7.34 PTSEE141 pKa = 4.3 VKK143 pKa = 10.91 SMSDD147 pKa = 3.11 EE148 pKa = 4.05 QFNILAKK155 pKa = 9.95 TYY157 pKa = 8.89 WQGLNDD163 pKa = 4.04 NGTLKK168 pKa = 10.61 DD169 pKa = 3.98 YY170 pKa = 11.28 KK171 pKa = 10.16 SANYY175 pKa = 10.1 GHH177 pKa = 6.95 VSTSDD182 pKa = 2.67 KK183 pKa = 10.76 GSRR186 pKa = 11.84 VNDD189 pKa = 3.49 FRR191 pKa = 11.84 AIGYY195 pKa = 5.02 TAKK198 pKa = 10.68 YY199 pKa = 8.41 VLKK202 pKa = 10.49 DD203 pKa = 2.85 IYY205 pKa = 9.79 TQQRR209 pKa = 11.84 DD210 pKa = 3.7 KK211 pKa = 11.2 FFKK214 pKa = 10.25 HH215 pKa = 6.05 HH216 pKa = 5.59 LAEE219 pKa = 4.59 YY220 pKa = 9.94 ISNEE224 pKa = 3.05 ISKK227 pKa = 11.02 YY228 pKa = 9.78 IDD230 pKa = 3.62 AGHH233 pKa = 6.9 EE234 pKa = 3.89 DD235 pKa = 3.51 FDD237 pKa = 5.12 FYY239 pKa = 11.4 KK240 pKa = 10.84 SPEE243 pKa = 4.06 YY244 pKa = 11.04 YY245 pKa = 8.53 IDD247 pKa = 3.81 KK248 pKa = 10.86 YY249 pKa = 11.16 GLHH252 pKa = 6.12 KK253 pKa = 9.55 TQDD256 pKa = 3.43 PYY258 pKa = 11.93 GWFHH262 pKa = 5.34 QCEE265 pKa = 4.62 VEE267 pKa = 3.95 WLRR270 pKa = 11.84 PILKK274 pKa = 10.12 LRR276 pKa = 11.84 FQTYY280 pKa = 9.38 YY281 pKa = 10.92 EE282 pKa = 4.34 LLSEE286 pKa = 4.23 YY287 pKa = 10.55 KK288 pKa = 10.2 NRR290 pKa = 11.84 YY291 pKa = 4.97 STKK294 pKa = 10.52 PMFSHH299 pKa = 7.01 NLGLYY304 pKa = 10.29 ALDD307 pKa = 4.1 FVTPLGKK314 pKa = 10.15 VKK316 pKa = 10.65 YY317 pKa = 9.78 YY318 pKa = 10.83 SNNNICYY325 pKa = 7.98 VTLPLYY331 pKa = 10.56 LYY333 pKa = 10.52 RR334 pKa = 11.84 KK335 pKa = 8.07 FFCTVEE341 pKa = 3.8 KK342 pKa = 10.48 SQYY345 pKa = 11.07 NFTINKK351 pKa = 8.54 NGKK354 pKa = 9.33 KK355 pKa = 9.46 IYY357 pKa = 10.25 NNIYY361 pKa = 9.0 VHH363 pKa = 6.11 TKK365 pKa = 9.82 QGIEE369 pKa = 3.56 LLYY372 pKa = 10.76 RR373 pKa = 11.84 QFLYY377 pKa = 10.98 KK378 pKa = 10.25 LDD380 pKa = 3.95 LSQNVLSALIPTLQNSKK397 pKa = 10.76 LVDD400 pKa = 3.43 EE401 pKa = 5.96 FINFQKK407 pKa = 10.8 EE408 pKa = 3.45 KK409 pKa = 10.27 GIYY412 pKa = 9.5 YY413 pKa = 7.38 EE414 pKa = 4.49 TSTSTVIKK422 pKa = 10.26 KK423 pKa = 8.97 LQSYY427 pKa = 6.96 EE428 pKa = 3.81 FRR430 pKa = 11.84 HH431 pKa = 6.79 SYY433 pKa = 10.31 FLYY436 pKa = 9.36 NYY438 pKa = 9.21 LYY440 pKa = 10.34 KK441 pKa = 10.71 GRR443 pKa = 11.84 YY444 pKa = 7.63 FPKK447 pKa = 10.22 TNTNKK452 pKa = 8.9 LTYY455 pKa = 10.54 NEE457 pKa = 3.96 INNYY461 pKa = 9.75 YY462 pKa = 10.62 SVGNLDD468 pKa = 3.46 IYY470 pKa = 10.54 TDD472 pKa = 3.99 FVRR475 pKa = 11.84 FHH477 pKa = 7.01 TYY479 pKa = 10.41 NEE481 pKa = 4.38 CVTTKK486 pKa = 10.07 QRR488 pKa = 11.84 QSDD491 pKa = 3.38 IFEE494 pKa = 4.76 SYY496 pKa = 11.37 DD497 pKa = 3.3 VFPPFAQNLHH507 pKa = 6.11 IYY509 pKa = 9.92 CWIDD513 pKa = 3.47 DD514 pKa = 4.04 FQHH517 pKa = 6.99 FILSQSDD524 pKa = 3.64 EE525 pKa = 3.9 RR526 pKa = 11.84 SKK528 pKa = 11.43 AIFYY532 pKa = 9.46 EE533 pKa = 4.3 KK534 pKa = 10.52 ARR536 pKa = 11.84 CRR538 pKa = 11.84 RR539 pKa = 11.84 NLKK542 pKa = 9.35 PLKK545 pKa = 10.35 KK546 pKa = 9.64 YY547 pKa = 10.94 LCVV550 pKa = 3.39
Molecular weight: 66.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.607
IPC2_protein 8.653
IPC_protein 8.492
Toseland 8.873
ProMoST 8.902
Dawson 9.297
Bjellqvist 9.165
Wikipedia 9.502
Rodwell 9.399
Grimsley 9.37
Solomon 9.311
Lehninger 9.253
Nozaki 9.136
DTASelect 9.092
Thurlkill 9.136
EMBOSS 9.385
Sillero 9.297
Patrickios 3.478
IPC_peptide 9.297
IPC2_peptide 7.892
IPC2.peptide.svr19 7.757
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1646
101
586
411.5
47.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.739 ± 1.781
1.094 ± 0.375
5.893 ± 0.693
5.772 ± 1.042
6.075 ± 0.793
5.832 ± 0.99
1.944 ± 0.344
5.225 ± 0.329
7.23 ± 1.151
8.688 ± 0.164
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.187 ± 0.366
6.865 ± 0.53
3.949 ± 0.584
4.496 ± 0.286
4.374 ± 0.233
7.412 ± 0.497
5.711 ± 0.345
5.164 ± 0.79
0.972 ± 0.207
6.379 ± 1.785
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here