Rhodobacter sp. ETT8

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodobacter; unclassified Rhodobacter

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4261 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B3RZ10|A0A6B3RZ10_9RHOB Uncharacterized protein OS=Rhodobacter sp. ETT8 OX=2709381 GN=G3572_19455 PE=4 SV=1
MM1 pKa = 7.69LGGLVALPVAVSAHH15 pKa = 4.73PHH17 pKa = 4.76VFIDD21 pKa = 3.44ATIEE25 pKa = 4.19VIFDD29 pKa = 3.36AEE31 pKa = 4.11GRR33 pKa = 11.84AEE35 pKa = 3.97ALRR38 pKa = 11.84IGWRR42 pKa = 11.84YY43 pKa = 10.84DD44 pKa = 3.09SLFSMTYY51 pKa = 9.9VAEE54 pKa = 4.03QGYY57 pKa = 10.44DD58 pKa = 3.41PDD60 pKa = 4.28FDD62 pKa = 3.98GVLTAEE68 pKa = 4.57EE69 pKa = 4.06EE70 pKa = 3.97AALAGFDD77 pKa = 4.0MGWHH81 pKa = 6.65EE82 pKa = 5.6DD83 pKa = 3.89FAGDD87 pKa = 3.93TYY89 pKa = 11.92ALLGEE94 pKa = 4.75APLALSGPLEE104 pKa = 4.04PTAAYY109 pKa = 10.65VDD111 pKa = 3.68GSLRR115 pKa = 11.84STHH118 pKa = 5.9LRR120 pKa = 11.84RR121 pKa = 11.84LAAPVALGAEE131 pKa = 4.18EE132 pKa = 5.57LVVQVYY138 pKa = 10.43DD139 pKa = 3.6PSFYY143 pKa = 10.34TSYY146 pKa = 11.11LIAEE150 pKa = 4.58TPVLTGGEE158 pKa = 4.29GCRR161 pKa = 11.84VQVYY165 pKa = 9.94EE166 pKa = 4.58PDD168 pKa = 3.62RR169 pKa = 11.84EE170 pKa = 4.17AADD173 pKa = 3.7ARR175 pKa = 11.84LQAALDD181 pKa = 4.03EE182 pKa = 4.76LAGSADD188 pKa = 3.73AEE190 pKa = 4.71SEE192 pKa = 4.4FPAIGAAYY200 pKa = 9.98AEE202 pKa = 4.6EE203 pKa = 4.26ARR205 pKa = 11.84ISCGGEE211 pKa = 3.53

Molecular weight: 22.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B3RLJ2|A0A6B3RLJ2_9RHOB DNA helicase OS=Rhodobacter sp. ETT8 OX=2709381 GN=recQ PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34GGRR28 pKa = 11.84LVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.21GRR39 pKa = 11.84HH40 pKa = 5.51KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4261

0

4261

1351082

31

9718

317.1

34.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.517 ± 0.061

0.847 ± 0.013

5.667 ± 0.034

5.471 ± 0.035

3.596 ± 0.022

8.874 ± 0.048

2.022 ± 0.019

4.837 ± 0.027

2.717 ± 0.035

10.379 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.678 ± 0.024

2.358 ± 0.03

5.361 ± 0.033

3.225 ± 0.02

7.193 ± 0.045

4.953 ± 0.032

5.417 ± 0.042

7.41 ± 0.037

1.467 ± 0.018

2.011 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski