Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2028 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7MQZ0|Q7MQZ0_WOLSU Uncharacterized protein OS=Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) OX=273121 GN=WS1887 PE=4 SV=1
MM1 pKa = 8.05 SLMITEE7 pKa = 4.24 EE8 pKa = 4.63 CIACDD13 pKa = 3.6 ACRR16 pKa = 11.84 EE17 pKa = 4.11 EE18 pKa = 5.06 CPNEE22 pKa = 4.57 AIDD25 pKa = 4.79 EE26 pKa = 4.46 GDD28 pKa = 3.67 PTYY31 pKa = 10.55 MIDD34 pKa = 3.57 PDD36 pKa = 3.73 RR37 pKa = 11.84 CTEE40 pKa = 3.94 CVGYY44 pKa = 10.39 YY45 pKa = 10.14 DD46 pKa = 4.76 EE47 pKa = 5.24 PSCVGACPVDD57 pKa = 5.22 AIIPDD62 pKa = 4.07 PDD64 pKa = 3.39 NVEE67 pKa = 4.2 SVEE70 pKa = 3.9 EE71 pKa = 3.85 LKK73 pKa = 11.31 YY74 pKa = 10.84 KK75 pKa = 10.69 FEE77 pKa = 3.99 QLQNEE82 pKa = 4.54 EE83 pKa = 4.12
Molecular weight: 9.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.962
IPC2_protein 3.567
IPC_protein 3.503
Toseland 3.312
ProMoST 3.643
Dawson 3.478
Bjellqvist 3.706
Wikipedia 3.401
Rodwell 3.338
Grimsley 3.236
Solomon 3.452
Lehninger 3.401
Nozaki 3.617
DTASelect 3.745
Thurlkill 3.376
EMBOSS 3.414
Sillero 3.617
Patrickios 0.006
IPC_peptide 3.452
IPC2_peptide 3.592
IPC2.peptide.svr19 3.664
Protein with the highest isoelectric point:
>tr|Q7MA09|Q7MA09_WOLSU PUTATIVE TYPE II PROTEIN SECRETION E OS=Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) OX=273121 GN=WS0543 PE=3 SV=1
MM1 pKa = 7.81 RR2 pKa = 11.84 NRR4 pKa = 11.84 YY5 pKa = 8.98 IEE7 pKa = 4.4 SFEE10 pKa = 4.03 KK11 pKa = 10.92 AQIADD16 pKa = 3.53 KK17 pKa = 10.91 NIPQFKK23 pKa = 10.64 AGDD26 pKa = 3.75 TLRR29 pKa = 11.84 VGVKK33 pKa = 9.52 IQEE36 pKa = 4.05 GDD38 pKa = 3.07 KK39 pKa = 10.85 TRR41 pKa = 11.84 VQNFEE46 pKa = 4.43 GICISVRR53 pKa = 11.84 GTGTGKK59 pKa = 8.35 TFTIRR64 pKa = 11.84 KK65 pKa = 7.8 MGANNVGVEE74 pKa = 4.52 RR75 pKa = 11.84 IFPLYY80 pKa = 10.33 SDD82 pKa = 3.77 SLEE85 pKa = 4.28 SVTLLRR91 pKa = 11.84 VGRR94 pKa = 11.84 VRR96 pKa = 11.84 RR97 pKa = 11.84 AKK99 pKa = 10.34 LYY101 pKa = 9.03 YY102 pKa = 10.49 LRR104 pKa = 11.84 DD105 pKa = 3.64 RR106 pKa = 11.84 SGKK109 pKa = 7.29 SARR112 pKa = 11.84 IKK114 pKa = 9.98 EE115 pKa = 4.03 LRR117 pKa = 11.84 KK118 pKa = 10.02
Molecular weight: 13.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 9.736
IPC_protein 10.394
Toseland 10.774
ProMoST 10.438
Dawson 10.847
Bjellqvist 10.526
Wikipedia 11.038
Rodwell 11.169
Grimsley 10.891
Solomon 10.95
Lehninger 10.921
Nozaki 10.745
DTASelect 10.526
Thurlkill 10.76
EMBOSS 11.155
Sillero 10.774
Patrickios 10.906
IPC_peptide 10.965
IPC2_peptide 9.297
IPC2.peptide.svr19 8.708
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2028
0
2028
654246
33
2883
322.6
36.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.902 ± 0.051
0.943 ± 0.023
4.334 ± 0.038
8.274 ± 0.064
4.807 ± 0.041
7.111 ± 0.054
2.074 ± 0.025
7.103 ± 0.044
6.736 ± 0.052
11.548 ± 0.08
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.47 ± 0.024
3.427 ± 0.039
3.734 ± 0.038
3.187 ± 0.032
5.008 ± 0.042
6.944 ± 0.045
4.238 ± 0.041
5.877 ± 0.046
0.977 ± 0.02
3.305 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here