Peptoniphilus indolicus ATCC 29427

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Peptoniphilaceae; Peptoniphilus; Peptoniphilus indolicus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4D754|G4D754_9FIRM Uncharacterized protein OS=Peptoniphilus indolicus ATCC 29427 OX=997350 GN=HMPREF9129_2234 PE=4 SV=1
MM1 pKa = 7.72KK2 pKa = 10.28LQLFDD7 pKa = 5.34YY8 pKa = 9.87IDD10 pKa = 3.73KK11 pKa = 10.97SAEE14 pKa = 3.64LLNYY18 pKa = 8.88YY19 pKa = 10.48RR20 pKa = 11.84NILEE24 pKa = 4.82EE25 pKa = 3.82ISEE28 pKa = 4.42NIVDD32 pKa = 4.2YY33 pKa = 11.15LWDD36 pKa = 3.77SLDD39 pKa = 3.42NSVITNITT47 pKa = 3.14

Molecular weight: 5.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4D5X4|G4D5X4_9FIRM DNA topoisomerase 1 OS=Peptoniphilus indolicus ATCC 29427 OX=997350 GN=topA PE=3 SV=1
MM1 pKa = 7.96DD2 pKa = 6.06IIRR5 pKa = 11.84AIEE8 pKa = 4.05AEE10 pKa = 4.15QLRR13 pKa = 11.84DD14 pKa = 3.85DD15 pKa = 4.67LPSFNVGDD23 pKa = 4.18TVQVHH28 pKa = 4.7YY29 pKa = 10.64RR30 pKa = 11.84IIEE33 pKa = 3.99GSRR36 pKa = 11.84EE37 pKa = 3.47RR38 pKa = 11.84VQAFEE43 pKa = 4.08GTVIKK48 pKa = 10.58IQGKK52 pKa = 7.29SSRR55 pKa = 11.84KK56 pKa = 6.93TFTVRR61 pKa = 11.84RR62 pKa = 11.84ISYY65 pKa = 9.67GIGVEE70 pKa = 4.33RR71 pKa = 11.84TFLMNTPRR79 pKa = 11.84IAKK82 pKa = 9.27LVVVRR87 pKa = 11.84RR88 pKa = 11.84GKK90 pKa = 9.43VRR92 pKa = 11.84RR93 pKa = 11.84SKK95 pKa = 10.8LYY97 pKa = 10.11YY98 pKa = 10.44LRR100 pKa = 11.84DD101 pKa = 3.83RR102 pKa = 11.84QGKK105 pKa = 7.63AAKK108 pKa = 10.18VKK110 pKa = 10.33EE111 pKa = 4.18KK112 pKa = 11.21NNYY115 pKa = 8.35

Molecular weight: 13.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2269

0

2269

625768

37

2918

275.8

31.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.813 ± 0.055

0.888 ± 0.02

5.584 ± 0.046

8.014 ± 0.071

4.557 ± 0.044

6.489 ± 0.042

1.35 ± 0.021

9.293 ± 0.066

8.783 ± 0.054

9.213 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.728 ± 0.025

5.8 ± 0.04

2.768 ± 0.026

2.348 ± 0.022

3.677 ± 0.042

6.421 ± 0.042

4.872 ± 0.035

6.758 ± 0.039

0.594 ± 0.013

4.05 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski