Flavobacterium phage 6H

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Unahavirus; Flavobacterium virus 6H

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9VW81|R9VW81_9CAUD S49 family serine protease OS=Flavobacterium phage 6H OX=1325731 PE=4 SV=1
MM1 pKa = 8.06RR2 pKa = 11.84NRR4 pKa = 11.84IYY6 pKa = 10.25IQYY9 pKa = 9.89SGKK12 pKa = 8.17ITKK15 pKa = 8.21NTIALMEE22 pKa = 4.56TYY24 pKa = 10.11LICDD28 pKa = 4.78FIGRR32 pKa = 11.84TVIDD36 pKa = 3.57GRR38 pKa = 11.84RR39 pKa = 11.84AYY41 pKa = 9.61IDD43 pKa = 3.9LKK45 pKa = 10.97QSEE48 pKa = 4.73NPNLDD53 pKa = 3.37EE54 pKa = 4.11VSQFILALDD63 pKa = 3.84GTDD66 pKa = 6.66FITFWGYY73 pKa = 9.86PLEE76 pKa = 4.99DD77 pKa = 3.88GSNITTNEE85 pKa = 3.77DD86 pKa = 3.29FKK88 pKa = 11.48DD89 pKa = 4.53FSISEE94 pKa = 3.79IDD96 pKa = 3.12AA97 pKa = 4.2

Molecular weight: 11.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9VX02|R9VX02_9CAUD Uncharacterized protein OS=Flavobacterium phage 6H OX=1325731 PE=4 SV=1
MM1 pKa = 6.79QQQPTIKK8 pKa = 10.4LRR10 pKa = 11.84DD11 pKa = 4.03AIKK14 pKa = 10.7KK15 pKa = 8.45MRR17 pKa = 11.84ALTGINVPFSFSYY30 pKa = 10.2ISYY33 pKa = 10.62SEE35 pKa = 4.16VLNKK39 pKa = 10.35SEE41 pKa = 4.35GYY43 pKa = 10.05KK44 pKa = 10.08RR45 pKa = 11.84VNKK48 pKa = 10.1GLLRR52 pKa = 11.84AGYY55 pKa = 9.35RR56 pKa = 11.84ANQSEE61 pKa = 4.24RR62 pKa = 11.84SNILIAYY69 pKa = 8.87IDD71 pKa = 4.03YY72 pKa = 10.62DD73 pKa = 3.54KK74 pKa = 11.66KK75 pKa = 10.39EE76 pKa = 4.11ADD78 pKa = 3.69RR79 pKa = 11.84QFYY82 pKa = 10.85LPLLMTFNNIQVIPP96 pKa = 3.92

Molecular weight: 11.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13827

45

1198

219.5

25.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.576 ± 0.452

0.767 ± 0.097

5.583 ± 0.183

7.297 ± 0.223

5.214 ± 0.264

5.034 ± 0.227

1.62 ± 0.136

8.679 ± 0.276

9.438 ± 0.309

8.794 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.285 ± 0.16

7.008 ± 0.311

2.951 ± 0.162

3.638 ± 0.138

3.717 ± 0.175

6.495 ± 0.223

5.229 ± 0.25

5.308 ± 0.152

1.215 ± 0.117

4.151 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski