Bovine adenovirus B serotype 3 (BAdV-3) (Mastadenovirus bos3)
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q64844|Q64844_ADEB3 E1B 55 kDa protein OS=Bovine adenovirus B serotype 3 OX=10510 GN=E1B PE=3 SV=1
MM1 pKa = 7.74 KK2 pKa = 10.71 YY3 pKa = 10.36 LVLVLNDD10 pKa = 3.04 GMSRR14 pKa = 11.84 IEE16 pKa = 4.26 KK17 pKa = 10.54 ALLCSDD23 pKa = 4.1 GEE25 pKa = 4.28 VDD27 pKa = 4.85 LEE29 pKa = 4.28 CHH31 pKa = 5.66 EE32 pKa = 4.63 VLPPSPAPVPASVSPVRR49 pKa = 11.84 SPPPLSPVFPPSPPAPLVNPEE70 pKa = 4.23 ASSLLQQYY78 pKa = 9.58 RR79 pKa = 11.84 RR80 pKa = 11.84 EE81 pKa = 3.96 LLEE84 pKa = 4.36 RR85 pKa = 11.84 SLLRR89 pKa = 11.84 TAEE92 pKa = 4.09 GQQRR96 pKa = 11.84 AVCPCEE102 pKa = 3.68 RR103 pKa = 11.84 LPVEE107 pKa = 4.71 EE108 pKa = 5.3 DD109 pKa = 3.01 EE110 pKa = 5.32 CLNAVNLLFPDD121 pKa = 3.61 PWLNAAEE128 pKa = 4.14 NGGDD132 pKa = 3.43 IFKK135 pKa = 10.73 SPAMSPEE142 pKa = 3.52 PWIDD146 pKa = 3.35 LSSYY150 pKa = 11.05 DD151 pKa = 3.61 SDD153 pKa = 4.09 VEE155 pKa = 4.25 EE156 pKa = 4.49 VTSHH160 pKa = 7.13 FFLDD164 pKa = 4.22 CPEE167 pKa = 4.49 DD168 pKa = 3.75 PSRR171 pKa = 11.84 EE172 pKa = 3.94 CSSCGFHH179 pKa = 5.98 QAQSGIPGIMCSLCYY194 pKa = 9.77 MRR196 pKa = 11.84 QTYY199 pKa = 9.1 HH200 pKa = 7.14 CIYY203 pKa = 10.35 SPVSEE208 pKa = 4.51 EE209 pKa = 3.93 EE210 pKa = 3.93 MM211 pKa = 4.33
Molecular weight: 23.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.135
IPC2_protein 4.368
IPC_protein 4.279
Toseland 4.126
ProMoST 4.38
Dawson 4.228
Bjellqvist 4.368
Wikipedia 4.075
Rodwell 4.126
Grimsley 4.037
Solomon 4.215
Lehninger 4.177
Nozaki 4.329
DTASelect 4.457
Thurlkill 4.126
EMBOSS 4.101
Sillero 4.393
Patrickios 0.782
IPC_peptide 4.228
IPC2_peptide 4.38
IPC2.peptide.svr19 4.299
Protein with the highest isoelectric point:
>tr|O71100|O71100_ADEB3 PVI OS=Bovine adenovirus B serotype 3 OX=10510 PE=4 SV=1
MM1 pKa = 7.59 SPRR4 pKa = 11.84 GNLTYY9 pKa = 10.85 RR10 pKa = 11.84 LRR12 pKa = 11.84 IPVALSGRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 TGLRR28 pKa = 11.84 GGSAYY33 pKa = 11.06 LLGRR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 AGGGRR46 pKa = 11.84 LRR48 pKa = 11.84 GGFLPLLAPIIAAAIGAIPGIASVAIQAAHH78 pKa = 6.14 NKK80 pKa = 9.21
Molecular weight: 8.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.412
IPC2_protein 11.169
IPC_protein 12.486
Toseland 12.632
ProMoST 13.144
Dawson 12.632
Bjellqvist 12.632
Wikipedia 13.115
Rodwell 12.179
Grimsley 12.676
Solomon 13.144
Lehninger 13.042
Nozaki 12.632
DTASelect 12.632
Thurlkill 12.632
EMBOSS 13.144
Sillero 12.632
Patrickios 11.901
IPC_peptide 13.144
IPC2_peptide 12.135
IPC2.peptide.svr19 9.122
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24
4
28
10367
69
1023
370.3
41.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.228 ± 0.497
1.939 ± 0.424
4.688 ± 0.207
5.46 ± 0.469
4.138 ± 0.331
6.357 ± 0.641
2.45 ± 0.166
3.887 ± 0.244
3.482 ± 0.358
9.916 ± 0.497
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.122 ± 0.23
4.466 ± 0.499
6.945 ± 0.411
4.514 ± 0.23
7.254 ± 0.674
7.263 ± 0.35
5.865 ± 0.35
6.472 ± 0.408
1.254 ± 0.135
3.299 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here