Corynebacterium bovis
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2067 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3R8PJ20|A0A3R8PJ20_9CORY PE family protein (Fragment) OS=Corynebacterium bovis OX=36808 GN=CXF42_09965 PE=4 SV=1
MM1 pKa = 7.08 STRR4 pKa = 11.84 TYY6 pKa = 9.87 TVTGMSCGHH15 pKa = 6.78 CEE17 pKa = 3.61 NAVRR21 pKa = 11.84 EE22 pKa = 4.33 EE23 pKa = 4.08 VSALDD28 pKa = 3.72 GVSDD32 pKa = 3.77 VTVSAEE38 pKa = 4.14 TGTLTLDD45 pKa = 3.76 APDD48 pKa = 4.63 AVTDD52 pKa = 4.12 DD53 pKa = 4.02 AVVAAVDD60 pKa = 3.61 EE61 pKa = 4.72 AGYY64 pKa = 10.53 AATRR68 pKa = 11.84 AEE70 pKa = 4.1
Molecular weight: 7.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.859
IPC_protein 3.757
Toseland 3.567
ProMoST 3.884
Dawson 3.745
Bjellqvist 3.999
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.478
Solomon 3.719
Lehninger 3.681
Nozaki 3.884
DTASelect 4.062
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.884
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.823
Protein with the highest isoelectric point:
>tr|A0A3R8R3U4|A0A3R8R3U4_9CORY DUF2786 domain-containing protein OS=Corynebacterium bovis OX=36808 GN=CXF42_10735 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 5.99 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 SGRR31 pKa = 11.84 AIVSARR37 pKa = 11.84 RR38 pKa = 11.84 SKK40 pKa = 10.67 GRR42 pKa = 11.84 KK43 pKa = 9.06 SLTAA47 pKa = 4.07
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2067
0
2067
719965
33
4922
348.3
36.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.1 ± 0.091
0.715 ± 0.011
7.033 ± 0.047
5.214 ± 0.067
2.607 ± 0.033
10.14 ± 0.067
2.091 ± 0.023
3.444 ± 0.04
1.742 ± 0.045
8.927 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.875 ± 0.021
1.81 ± 0.027
5.995 ± 0.053
2.366 ± 0.029
7.998 ± 0.051
5.192 ± 0.041
6.946 ± 0.051
9.652 ± 0.065
1.356 ± 0.019
1.795 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here