Streptococcus phage Javan645
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6AQ19|A0A4D6AQ19_9CAUD Uncharacterized protein OS=Streptococcus phage Javan645 OX=2548298 GN=Javan645_0035 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.1 LWEE5 pKa = 4.73 YY6 pKa = 10.67 INKK9 pKa = 8.17 TVRR12 pKa = 11.84 LVLVDD17 pKa = 3.63 GTSVIGKK24 pKa = 9.22 VLDD27 pKa = 3.68 WYY29 pKa = 10.7 DD30 pKa = 3.97 GVDD33 pKa = 4.03 LDD35 pKa = 4.87 GDD37 pKa = 4.1 DD38 pKa = 4.55 EE39 pKa = 4.84 IVIEE43 pKa = 4.07 DD44 pKa = 3.49 HH45 pKa = 7.11 SYY47 pKa = 10.43 PEE49 pKa = 4.06 SSIKK53 pKa = 10.08 QIEE56 pKa = 4.63 VISTT60 pKa = 3.46
Molecular weight: 6.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.26
IPC2_protein 4.101
IPC_protein 4.012
Toseland 3.808
ProMoST 4.177
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.948
Rodwell 3.846
Grimsley 3.732
Solomon 3.986
Lehninger 3.935
Nozaki 4.126
DTASelect 4.342
Thurlkill 3.872
EMBOSS 3.948
Sillero 4.126
Patrickios 3.63
IPC_peptide 3.986
IPC2_peptide 4.113
IPC2.peptide.svr19 4.048
Protein with the highest isoelectric point:
>tr|A0A4D6AMU9|A0A4D6AMU9_9CAUD Uncharacterized protein OS=Streptococcus phage Javan645 OX=2548298 GN=Javan645_0042 PE=4 SV=1
MM1 pKa = 7.41 GATVSVKK8 pKa = 10.16 VDD10 pKa = 3.35 LKK12 pKa = 11.18 GIEE15 pKa = 4.4 KK16 pKa = 10.24 KK17 pKa = 10.92 VSSQALARR25 pKa = 11.84 GKK27 pKa = 10.21 LAIANQMILDD37 pKa = 4.19 MAPFTPAKK45 pKa = 9.71 EE46 pKa = 4.14 GNLRR50 pKa = 11.84 GSAQASGDD58 pKa = 3.72 SVRR61 pKa = 11.84 YY62 pKa = 7.34 PGPYY66 pKa = 10.06 ARR68 pKa = 11.84 AQFYY72 pKa = 10.01 GSSYY76 pKa = 10.76 NKK78 pKa = 9.95 HH79 pKa = 6.09 KK80 pKa = 11.06 SFTFKK85 pKa = 10.76 RR86 pKa = 11.84 YY87 pKa = 5.0 TTPGTGKK94 pKa = 10.26 RR95 pKa = 11.84 WDD97 pKa = 3.66 KK98 pKa = 10.93 KK99 pKa = 9.8 ATALHH104 pKa = 5.63 VKK106 pKa = 9.96 DD107 pKa = 3.3 WGKK110 pKa = 10.84 VGLRR114 pKa = 11.84 AMGVKK119 pKa = 10.0 AA120 pKa = 4.47
Molecular weight: 13.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.218
IPC2_protein 9.823
IPC_protein 9.984
Toseland 10.643
ProMoST 10.175
Dawson 10.745
Bjellqvist 10.35
Wikipedia 10.877
Rodwell 11.359
Grimsley 10.789
Solomon 10.774
Lehninger 10.76
Nozaki 10.599
DTASelect 10.35
Thurlkill 10.628
EMBOSS 11.008
Sillero 10.657
Patrickios 11.082
IPC_peptide 10.789
IPC2_peptide 8.858
IPC2.peptide.svr19 8.718
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
11163
44
1504
242.7
27.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.346 ± 1.323
0.421 ± 0.068
6.522 ± 0.524
6.539 ± 0.557
4.112 ± 0.231
6.28 ± 0.548
1.191 ± 0.17
7.122 ± 0.24
8.636 ± 0.557
8.116 ± 0.318
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.562 ± 0.307
6.154 ± 0.507
2.446 ± 0.239
4.049 ± 0.227
3.547 ± 0.298
6.835 ± 0.603
6.548 ± 0.44
6.566 ± 0.337
1.021 ± 0.139
3.986 ± 0.442
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here