Ureaplasma parvum serovar 3 (strain ATCC 700970)
Average proteome isoelectric point is 7.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 611 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9PR48|Q9PR48_UREPA Type I restriction enzyme S protein OS=Ureaplasma parvum serovar 3 (strain ATCC 700970) OX=273119 GN=hsdS-1 PE=4 SV=1
MM1 pKa = 7.64 AKK3 pKa = 10.15 YY4 pKa = 10.02 IKK6 pKa = 9.99 TGVSYY11 pKa = 11.14 INLDD15 pKa = 3.29 NARR18 pKa = 11.84 TINILPEE25 pKa = 5.56 DD26 pKa = 3.69 IDD28 pKa = 4.29 SYY30 pKa = 11.84 LEE32 pKa = 4.02 LGGDD36 pKa = 3.76 EE37 pKa = 4.69 AYY39 pKa = 8.02 KK40 pKa = 10.68 TSDD43 pKa = 4.0 LGSEE47 pKa = 4.66 LYY49 pKa = 10.29 INYY52 pKa = 10.15 ADD54 pKa = 4.33 FEE56 pKa = 4.76 STNILFDD63 pKa = 4.01 LKK65 pKa = 10.97 KK66 pKa = 10.85 EE67 pKa = 3.92 EE68 pKa = 4.13 LQAKK72 pKa = 9.33 IDD74 pKa = 3.8 AFLISNDD81 pKa = 3.48 TVLDD85 pKa = 3.98 LSEE88 pKa = 4.41 VFLDD92 pKa = 3.25 IHH94 pKa = 6.73 FSEE97 pKa = 5.91 DD98 pKa = 3.15 DD99 pKa = 4.49 DD100 pKa = 5.13 YY101 pKa = 12.11 EE102 pKa = 4.19 EE103 pKa = 5.32 DD104 pKa = 3.86 CCCEE108 pKa = 4.49 DD109 pKa = 3.62 EE110 pKa = 4.7 CCSDD114 pKa = 4.15 EE115 pKa = 5.16 EE116 pKa = 5.79 NEE118 pKa = 4.17 ACCNSEE124 pKa = 3.92 VKK126 pKa = 10.66 VEE128 pKa = 4.11 EE129 pKa = 4.47 EE130 pKa = 4.46 CCGGAKK136 pKa = 9.97 DD137 pKa = 4.12 DD138 pKa = 3.95 CCGGHH143 pKa = 4.63 EE144 pKa = 4.73 HH145 pKa = 5.82 EE146 pKa = 6.08 HH147 pKa = 5.86 EE148 pKa = 4.58 VCCDD152 pKa = 3.44 SEE154 pKa = 4.77 TKK156 pKa = 10.23 TSEE159 pKa = 4.18 TQEE162 pKa = 4.2 EE163 pKa = 5.47 CCGGTKK169 pKa = 10.11 DD170 pKa = 4.01 DD171 pKa = 3.91 CCGGHH176 pKa = 5.26 EE177 pKa = 4.56 HH178 pKa = 6.34 EE179 pKa = 4.85 HH180 pKa = 5.95 HH181 pKa = 6.68 HH182 pKa = 6.04 HH183 pKa = 7.0 HH184 pKa = 6.08 SHH186 pKa = 5.1 QHH188 pKa = 4.48
Molecular weight: 21.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.03
IPC2_protein 4.139
IPC_protein 4.113
Toseland 3.935
ProMoST 4.228
Dawson 4.075
Bjellqvist 4.228
Wikipedia 3.948
Rodwell 3.948
Grimsley 3.846
Solomon 4.075
Lehninger 4.024
Nozaki 4.177
DTASelect 4.342
Thurlkill 3.961
EMBOSS 3.973
Sillero 4.228
Patrickios 0.324
IPC_peptide 4.075
IPC2_peptide 4.215
IPC2.peptide.svr19 4.12
Protein with the highest isoelectric point:
>sp|Q9PPN8|RSMA_UREPA Ribosomal RNA small subunit methyltransferase A OS=Ureaplasma parvum serovar 3 (strain ATCC 700970) OX=273119 GN=rsmA PE=3 SV=2
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 8.78 RR12 pKa = 11.84 AKK14 pKa = 9.29 VHH16 pKa = 5.53 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MKK23 pKa = 9.24 TKK25 pKa = 10.3 NGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.53 GRR39 pKa = 11.84 HH40 pKa = 5.44 SLTVSSEE47 pKa = 4.0 KK48 pKa = 10.94
Molecular weight: 5.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.406
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.369
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.055
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
611
0
611
227764
34
5005
372.8
42.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.902 ± 0.087
0.664 ± 0.03
5.681 ± 0.078
5.756 ± 0.118
5.16 ± 0.076
4.133 ± 0.099
1.733 ± 0.034
10.323 ± 0.112
9.949 ± 0.089
10.003 ± 0.097
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.686 ± 0.058
9.069 ± 0.217
2.655 ± 0.055
3.835 ± 0.056
2.768 ± 0.074
5.989 ± 0.065
5.018 ± 0.085
5.36 ± 0.071
0.872 ± 0.027
4.443 ± 0.062
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here