Stenotrophomonas phage SMA7

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Subteminivirus; Stenotrophomonas virus SMA7

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0F3B2|S0F3B2_9VIRU DNA-Binding protein G5P OS=Stenotrophomonas phage SMA7 OX=1343494 PE=3 SV=1
MM1 pKa = 7.84DD2 pKa = 4.39FSGVFLGLSVAQVVAAIVGAGTLMALPWFGRR33 pKa = 11.84WCVDD37 pKa = 3.77KK38 pKa = 11.02IAGFFEE44 pKa = 4.74DD45 pKa = 6.23RR46 pKa = 11.84EE47 pKa = 4.18DD48 pKa = 3.84QDD50 pKa = 4.22ADD52 pKa = 3.34EE53 pKa = 5.16HH54 pKa = 7.98ADD56 pKa = 3.69DD57 pKa = 4.03EE58 pKa = 4.86AGEE61 pKa = 4.2VEE63 pKa = 4.62EE64 pKa = 5.09VVCADD69 pKa = 3.67TGHH72 pKa = 7.3DD73 pKa = 3.91YY74 pKa = 11.55DD75 pKa = 4.54GGEE78 pKa = 5.27CIFCGQPEE86 pKa = 4.28KK87 pKa = 11.04EE88 pKa = 4.33DD89 pKa = 3.43

Molecular weight: 9.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0F2J4|S0F2J4_9VIRU Uncharacterized protein OS=Stenotrophomonas phage SMA7 OX=1343494 PE=4 SV=1
MM1 pKa = 6.91TAVNEE6 pKa = 4.11LLDD9 pKa = 3.77NVKK12 pKa = 9.84IRR14 pKa = 11.84LNLASDD20 pKa = 3.35MALAEE25 pKa = 4.27RR26 pKa = 11.84LTVTRR31 pKa = 11.84SLVSRR36 pKa = 11.84WRR38 pKa = 11.84KK39 pKa = 9.88GDD41 pKa = 3.38TPLADD46 pKa = 3.3EE47 pKa = 5.94RR48 pKa = 11.84IAQICALAKK57 pKa = 10.38LDD59 pKa = 3.97GPTWIAMIHH68 pKa = 6.07AEE70 pKa = 4.18RR71 pKa = 11.84ATSATEE77 pKa = 3.63RR78 pKa = 11.84ALWRR82 pKa = 11.84LMLDD86 pKa = 3.38RR87 pKa = 11.84MSAAAAVVALVALSMPGLANAKK109 pKa = 7.35TAQIQAVSAADD120 pKa = 3.47NGGMYY125 pKa = 10.51IMFKK129 pKa = 10.6AIRR132 pKa = 11.84RR133 pKa = 11.84WLARR137 pKa = 11.84ILPRR141 pKa = 11.84SPALLWHH148 pKa = 6.71GAA150 pKa = 3.36

Molecular weight: 16.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

1816

45

430

181.6

19.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.106 ± 1.056

1.652 ± 0.473

5.176 ± 1.109

5.396 ± 0.857

3.304 ± 0.703

7.985 ± 0.61

1.762 ± 0.515

4.46 ± 0.495

3.91 ± 0.811

8.48 ± 0.894

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.533 ± 0.615

1.872 ± 0.389

5.066 ± 0.55

3.414 ± 0.296

7.93 ± 1.309

5.672 ± 0.606

5.947 ± 0.809

8.15 ± 0.801

2.203 ± 0.376

1.982 ± 0.46
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski