Hymenobacter swuensis DY53
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4612 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8EY20|W8EY20_9BACT Uncharacterized protein OS=Hymenobacter swuensis DY53 OX=1227739 GN=Hsw_1077 PE=4 SV=1
MM1 pKa = 6.89 STTYY5 pKa = 10.34 PFLVADD11 pKa = 5.27 LEE13 pKa = 4.46 DD14 pKa = 3.42 QATPGRR20 pKa = 11.84 QQRR23 pKa = 11.84 LLVNLSNMDD32 pKa = 3.23 DD33 pKa = 3.96 YY34 pKa = 12.11 YY35 pKa = 11.91 NVFEE39 pKa = 4.99 KK40 pKa = 11.2 YY41 pKa = 10.41 GFSGSGASWAEE52 pKa = 3.91 HH53 pKa = 6.34 IEE55 pKa = 4.51 TILEE59 pKa = 3.87 EE60 pKa = 4.22 HH61 pKa = 6.77 APALLDD67 pKa = 3.51 HH68 pKa = 6.97 VEE70 pKa = 4.35 LLGQGEE76 pKa = 4.41 IFGVYY81 pKa = 9.23 TDD83 pKa = 4.6 GPGATEE89 pKa = 3.85 QFLALIQPIFGDD101 pKa = 3.81 LGSLSKK107 pKa = 10.88 YY108 pKa = 10.32 LSQADD113 pKa = 3.41 PGDD116 pKa = 3.6 FFEE119 pKa = 5.17
Molecular weight: 13.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.776
IPC2_protein 3.973
IPC_protein 3.91
Toseland 3.719
ProMoST 4.012
Dawson 3.884
Bjellqvist 4.088
Wikipedia 3.795
Rodwell 3.745
Grimsley 3.63
Solomon 3.872
Lehninger 3.821
Nozaki 3.999
DTASelect 4.177
Thurlkill 3.757
EMBOSS 3.808
Sillero 4.024
Patrickios 0.896
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.945
Protein with the highest isoelectric point:
>tr|W8EUR5|W8EUR5_9BACT Peptidase_M48 domain-containing protein OS=Hymenobacter swuensis DY53 OX=1227739 GN=Hsw_1339 PE=4 SV=1
MM1 pKa = 7.81 KK2 pKa = 9.73 IRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 PIGPVRR14 pKa = 11.84 GINPKK19 pKa = 9.79 KK20 pKa = 10.45 APPAGARR27 pKa = 11.84 GAFRR31 pKa = 11.84 KK32 pKa = 10.14 CNSITT37 pKa = 3.34
Molecular weight: 4.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 10.73
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.193
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.945
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.113
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4612
0
4612
1509703
37
2493
327.3
36.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.394 ± 0.043
0.724 ± 0.011
4.781 ± 0.026
5.337 ± 0.04
4.087 ± 0.022
7.648 ± 0.04
2.066 ± 0.021
4.23 ± 0.031
3.769 ± 0.038
11.153 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.86 ± 0.016
3.694 ± 0.036
5.276 ± 0.025
4.894 ± 0.03
6.272 ± 0.033
5.408 ± 0.031
6.486 ± 0.041
7.169 ± 0.034
1.283 ± 0.016
3.468 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here