Candidatus Magnetobacterium bavaricum
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6447 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F3GVL4|A0A0F3GVL4_9BACT Uncharacterized protein OS=Candidatus Magnetobacterium bavaricum OX=29290 GN=MBAV_001914 PE=4 SV=1
MM1 pKa = 7.74 HH2 pKa = 7.92 DD3 pKa = 4.4 IISLSLQDD11 pKa = 3.95 YY12 pKa = 9.4 PDD14 pKa = 3.86 VVGDD18 pKa = 4.83 DD19 pKa = 3.82 YY20 pKa = 10.7 WTSTDD25 pKa = 2.99 SDD27 pKa = 3.59 IYY29 pKa = 9.8 DD30 pKa = 3.54 TFVIGMSSGFVSSSSRR46 pKa = 11.84 TYY48 pKa = 10.59 PNSVWPVRR56 pKa = 11.84 SGQQ59 pKa = 3.25
Molecular weight: 6.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.757
IPC_protein 3.681
Toseland 3.452
ProMoST 3.923
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.77
Rodwell 3.516
Grimsley 3.376
Solomon 3.694
Lehninger 3.643
Nozaki 3.884
DTASelect 4.19
Thurlkill 3.579
EMBOSS 3.77
Sillero 3.821
Patrickios 0.604
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.749
Protein with the highest isoelectric point:
>tr|A0A0F3GU45|A0A0F3GU45_9BACT Uncharacterized protein OS=Candidatus Magnetobacterium bavaricum OX=29290 GN=MBAV_003569 PE=4 SV=1
MM1 pKa = 7.73 EE2 pKa = 5.5 PFSYY6 pKa = 10.3 QPRR9 pKa = 11.84 HH10 pKa = 6.21 CSHH13 pKa = 6.46 CRR15 pKa = 11.84 CRR17 pKa = 11.84 IGGDD21 pKa = 3.13 KK22 pKa = 10.77 RR23 pKa = 11.84 QGSQSARR30 pKa = 11.84 TQCAARR36 pKa = 11.84 VKK38 pKa = 9.8 PKK40 pKa = 9.98 PSKK43 pKa = 8.72 PQQRR47 pKa = 11.84 PTQDD51 pKa = 2.54 RR52 pKa = 11.84 QGQVVRR58 pKa = 11.84 GHH60 pKa = 5.88 VFPSKK65 pKa = 10.85 APAFAHH71 pKa = 7.14 DD72 pKa = 4.24 NEE74 pKa = 5.14 CGHH77 pKa = 6.71 GGHH80 pKa = 6.59 CRR82 pKa = 11.84 VYY84 pKa = 9.41 MHH86 pKa = 7.39 DD87 pKa = 3.48 RR88 pKa = 11.84 TSGKK92 pKa = 9.51 VQGTEE97 pKa = 3.75 LCHH100 pKa = 5.99 YY101 pKa = 8.15 PAAAPHH107 pKa = 6.59 PMAQGRR113 pKa = 11.84 IHH115 pKa = 6.91 KK116 pKa = 8.66 RR117 pKa = 11.84 QPQRR121 pKa = 11.84 RR122 pKa = 11.84 KK123 pKa = 7.71 QQQGRR128 pKa = 11.84 EE129 pKa = 4.01 LHH131 pKa = 6.3 PLGKK135 pKa = 8.57 RR136 pKa = 11.84 TRR138 pKa = 11.84 YY139 pKa = 7.97 QRR141 pKa = 11.84 RR142 pKa = 11.84 RR143 pKa = 11.84 YY144 pKa = 9.19 DD145 pKa = 3.29 RR146 pKa = 11.84 KK147 pKa = 10.1 HH148 pKa = 5.92 PLIHH152 pKa = 7.63 HH153 pKa = 6.42 EE154 pKa = 4.05 EE155 pKa = 4.32 QVWNRR160 pKa = 11.84 LCVIRR165 pKa = 11.84 RR166 pKa = 11.84 LLSDD170 pKa = 3.9 RR171 pKa = 11.84 VQPKK175 pKa = 8.77 PRR177 pKa = 11.84 KK178 pKa = 9.51 RR179 pKa = 11.84 PNHH182 pKa = 5.91 PANVRR187 pKa = 11.84 PKK189 pKa = 9.94 GQTITPQHH197 pKa = 6.97 PYY199 pKa = 8.43 HH200 pKa = 6.84 TDD202 pKa = 2.91 HH203 pKa = 6.51 AQHH206 pKa = 6.16 YY207 pKa = 8.48 KK208 pKa = 10.42 AVHH211 pKa = 5.87 NSAKK215 pKa = 10.43 DD216 pKa = 3.51 VLFSDD221 pKa = 4.28 HH222 pKa = 6.92 PAVEE226 pKa = 4.62 KK227 pKa = 10.68 SQSGGHH233 pKa = 5.16 QHH235 pKa = 5.98 HH236 pKa = 6.3 QCGTYY241 pKa = 9.65 QEE243 pKa = 4.69 PGGIAGVNLEE253 pKa = 4.13 HH254 pKa = 7.31 LLWCLRR260 pKa = 11.84 GQGRR264 pKa = 11.84 RR265 pKa = 11.84 AA266 pKa = 3.14
Molecular weight: 30.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.692
IPC_protein 10.467
Toseland 10.687
ProMoST 10.379
Dawson 10.789
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 11.008
Grimsley 10.833
Solomon 10.891
Lehninger 10.862
Nozaki 10.701
DTASelect 10.496
Thurlkill 10.687
EMBOSS 11.096
Sillero 10.73
Patrickios 10.716
IPC_peptide 10.906
IPC2_peptide 9.736
IPC2.peptide.svr19 8.519
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6447
0
6447
1584456
22
2585
245.8
27.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.179 ± 0.036
1.302 ± 0.015
5.931 ± 0.031
5.714 ± 0.043
4.106 ± 0.028
7.058 ± 0.041
1.924 ± 0.017
7.537 ± 0.034
6.315 ± 0.036
9.37 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.725 ± 0.015
4.446 ± 0.029
3.829 ± 0.024
3.084 ± 0.017
5.009 ± 0.036
6.508 ± 0.037
6.038 ± 0.05
7.206 ± 0.03
1.101 ± 0.021
3.616 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here