Eidolon helvum papillomavirus 3
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8YVV7|A0A1P8YVV7_9PAPI Regulatory protein E2 OS=Eidolon helvum papillomavirus 3 OX=1335477 GN=E2 PE=3 SV=1
MM1 pKa = 7.41 HH2 pKa = 6.97 PAKK5 pKa = 10.15 RR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 VPRR11 pKa = 11.84 DD12 pKa = 3.01 SAEE15 pKa = 3.93 NIYY18 pKa = 10.51 RR19 pKa = 11.84 NCIHH23 pKa = 7.18 FGTCPPDD30 pKa = 3.41 VVNKK34 pKa = 10.4 IEE36 pKa = 4.33 GKK38 pKa = 8.15 TVADD42 pKa = 6.06 KK43 pKa = 10.81 ILQWGSAGVFLGNLGISTGKK63 pKa = 10.75 GSANPLGYY71 pKa = 10.54 VPLGRR76 pKa = 11.84 GGGVGVGAWPRR87 pKa = 11.84 PGLATRR93 pKa = 11.84 PVVPTAPIEE102 pKa = 4.09 AVGPIDD108 pKa = 3.64 VGPAVVDD115 pKa = 3.68 PAVVDD120 pKa = 3.71 PVLIEE125 pKa = 4.23 MEE127 pKa = 5.37 PILPRR132 pKa = 11.84 HH133 pKa = 6.12 PEE135 pKa = 3.94 VVDD138 pKa = 4.48 EE139 pKa = 4.74 IVPIAPSDD147 pKa = 3.58 PSVISDD153 pKa = 3.23 NGVYY157 pKa = 9.31 PAHH160 pKa = 7.05 PAVIDD165 pKa = 3.84 EE166 pKa = 4.43 SLPTGGGRR174 pKa = 11.84 TVEE177 pKa = 4.44 VVAEE181 pKa = 4.07 VHH183 pKa = 6.67 HH184 pKa = 6.49 PADD187 pKa = 4.14 YY188 pKa = 10.79 FPVHH192 pKa = 6.93 PIDD195 pKa = 3.96 TGGSDD200 pKa = 3.61 NPAIIDD206 pKa = 3.63 VGEE209 pKa = 5.33 DD210 pKa = 3.02 IPLLPRR216 pKa = 11.84 TSTPHH221 pKa = 5.65 HH222 pKa = 6.79 TDD224 pKa = 2.94 PAILHH229 pKa = 5.99 ATTSFGTSAEE239 pKa = 4.27 VGGARR244 pKa = 11.84 YY245 pKa = 7.61 TVVDD249 pKa = 5.46 FEE251 pKa = 4.4 ATDD254 pKa = 3.67 PFVVEE259 pKa = 4.82 EE260 pKa = 4.51 GDD262 pKa = 4.1 IPLQVYY268 pKa = 10.06 DD269 pKa = 4.78 RR270 pKa = 11.84 EE271 pKa = 4.2 ADD273 pKa = 3.63 LEE275 pKa = 4.34 FRR277 pKa = 11.84 TSTPKK282 pKa = 10.52 EE283 pKa = 3.67 RR284 pKa = 11.84 TNPIRR289 pKa = 11.84 KK290 pKa = 8.87 VKK292 pKa = 10.35 ALYY295 pKa = 10.35 NRR297 pKa = 11.84 FVKK300 pKa = 9.93 QVPVEE305 pKa = 4.39 DD306 pKa = 4.54 PLFLTAPGEE315 pKa = 3.93 LVEE318 pKa = 5.86 YY319 pKa = 10.45 GFQNPAYY326 pKa = 9.99 DD327 pKa = 3.86 PDD329 pKa = 3.69 EE330 pKa = 4.56 TIEE333 pKa = 4.12 FSIDD337 pKa = 3.11 TDD339 pKa = 3.76 QPTAAPDD346 pKa = 3.27 SRR348 pKa = 11.84 FRR350 pKa = 11.84 DD351 pKa = 3.35 VHH353 pKa = 4.88 TLHH356 pKa = 6.74 RR357 pKa = 11.84 QILGEE362 pKa = 4.2 APTGHH367 pKa = 5.62 VRR369 pKa = 11.84 VSRR372 pKa = 11.84 VGSVAAMRR380 pKa = 11.84 TRR382 pKa = 11.84 SGATVGPQLHH392 pKa = 7.09 LYY394 pKa = 10.26 KK395 pKa = 10.41 DD396 pKa = 3.25 ISSIGEE402 pKa = 4.26 AIEE405 pKa = 4.17 LQPLSYY411 pKa = 10.26 PHH413 pKa = 7.07 ASDD416 pKa = 3.19 SSTYY420 pKa = 11.43 AVMPADD426 pKa = 3.56 TSFGGDD432 pKa = 3.44 VAVGAPPDD440 pKa = 3.43 VSTEE444 pKa = 3.96 VSSLEE449 pKa = 3.98 EE450 pKa = 4.18 GFEE453 pKa = 4.2 EE454 pKa = 5.21 VPLLQDD460 pKa = 4.47 HH461 pKa = 7.04 SGQQQTVGVSRR472 pKa = 11.84 GGSRR476 pKa = 11.84 DD477 pKa = 3.67 RR478 pKa = 11.84 QWITFEE484 pKa = 3.89 IPTPRR489 pKa = 11.84 TNIPASASSDD499 pKa = 2.92 RR500 pKa = 11.84 DD501 pKa = 3.19 IFVYY505 pKa = 10.78 YY506 pKa = 9.78 PDD508 pKa = 4.3 EE509 pKa = 4.64 SQDD512 pKa = 3.37 HH513 pKa = 6.89 DD514 pKa = 4.38 SSGEE518 pKa = 3.78 AAIDD522 pKa = 3.61 TDD524 pKa = 3.64 ITPARR529 pKa = 11.84 PIEE532 pKa = 4.64 PISPVGPSYY541 pKa = 11.4 VEE543 pKa = 4.15 STDD546 pKa = 3.86 SFPYY550 pKa = 9.38 WLAPSLQRR558 pKa = 11.84 KK559 pKa = 7.11 KK560 pKa = 10.59 RR561 pKa = 11.84 KK562 pKa = 9.12 RR563 pKa = 11.84 KK564 pKa = 7.12 TVSFCSLADD573 pKa = 3.4 GCMDD577 pKa = 3.54 SS578 pKa = 4.28
Molecular weight: 62.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.72
IPC2_protein 4.698
IPC_protein 4.685
Toseland 4.546
ProMoST 4.813
Dawson 4.66
Bjellqvist 4.8
Wikipedia 4.546
Rodwell 4.546
Grimsley 4.469
Solomon 4.66
Lehninger 4.609
Nozaki 4.762
DTASelect 4.965
Thurlkill 4.558
EMBOSS 4.571
Sillero 4.825
Patrickios 3.91
IPC_peptide 4.66
IPC2_peptide 4.813
IPC2.peptide.svr19 4.763
Protein with the highest isoelectric point:
>tr|A0A1P8YVV7|A0A1P8YVV7_9PAPI Regulatory protein E2 OS=Eidolon helvum papillomavirus 3 OX=1335477 GN=E2 PE=3 SV=1
MM1 pKa = 7.11 EE2 pKa = 5.66 ALRR5 pKa = 11.84 EE6 pKa = 4.07 RR7 pKa = 11.84 FDD9 pKa = 3.54 VLQEE13 pKa = 4.52 RR14 pKa = 11.84 ILKK17 pKa = 10.18 HH18 pKa = 6.19 YY19 pKa = 10.29 EE20 pKa = 3.67 LDD22 pKa = 3.77 SQKK25 pKa = 10.93 LADD28 pKa = 3.58 HH29 pKa = 6.57 CEE31 pKa = 3.49 YY32 pKa = 9.96 WRR34 pKa = 11.84 AVRR37 pKa = 11.84 KK38 pKa = 9.83 EE39 pKa = 3.68 NALLHH44 pKa = 5.0 YY45 pKa = 10.75 ARR47 pKa = 11.84 DD48 pKa = 3.8 KK49 pKa = 11.01 GVKK52 pKa = 8.43 TLGHH56 pKa = 5.98 MPVPAAQVSQARR68 pKa = 11.84 ARR70 pKa = 11.84 DD71 pKa = 4.06 AIAMQLAAEE80 pKa = 4.17 SLEE83 pKa = 3.96 RR84 pKa = 11.84 SGFGAEE90 pKa = 3.77 PWSLQEE96 pKa = 4.51 LSRR99 pKa = 11.84 EE100 pKa = 4.41 AYY102 pKa = 7.61 TAAPRR107 pKa = 11.84 NCFKK111 pKa = 10.97 KK112 pKa = 10.08 RR113 pKa = 11.84 GYY115 pKa = 8.42 TVLVMYY121 pKa = 10.52 DD122 pKa = 3.46 NSEE125 pKa = 4.32 EE126 pKa = 3.98 NCMEE130 pKa = 3.84 YY131 pKa = 10.2 TGWEE135 pKa = 4.19 DD136 pKa = 4.24 IYY138 pKa = 11.45 VQEE141 pKa = 6.12 DD142 pKa = 3.49 DD143 pKa = 5.61 DD144 pKa = 3.91 VWLKK148 pKa = 11.26 VKK150 pKa = 10.59 GLVDD154 pKa = 3.76 IYY156 pKa = 11.12 GLYY159 pKa = 10.32 YY160 pKa = 10.33 EE161 pKa = 4.81 SEE163 pKa = 4.28 GLKK166 pKa = 10.13 EE167 pKa = 4.1 YY168 pKa = 10.97 YY169 pKa = 8.95 VTFAQDD175 pKa = 2.86 AEE177 pKa = 4.84 TYY179 pKa = 10.49 SKK181 pKa = 9.92 TGLYY185 pKa = 7.43 TVKK188 pKa = 10.57 YY189 pKa = 9.1 KK190 pKa = 11.08 SEE192 pKa = 4.05 VLTNFHH198 pKa = 6.88 PVSSSTPRR206 pKa = 11.84 SPRR209 pKa = 11.84 ARR211 pKa = 11.84 RR212 pKa = 11.84 QRR214 pKa = 11.84 EE215 pKa = 3.97 EE216 pKa = 4.33 SPGSLPAGQGEE227 pKa = 4.84 GEE229 pKa = 4.5 GEE231 pKa = 4.47 GAQTPPSKK239 pKa = 10.2 RR240 pKa = 11.84 RR241 pKa = 11.84 RR242 pKa = 11.84 GLPDD246 pKa = 2.95 QGGSPTRR253 pKa = 11.84 SSNPEE258 pKa = 3.75 RR259 pKa = 11.84 RR260 pKa = 11.84 WPGQGQRR267 pKa = 11.84 QGEE270 pKa = 4.65 GEE272 pKa = 4.27 GGRR275 pKa = 11.84 PSQSPTRR282 pKa = 11.84 GSAGRR287 pKa = 11.84 APRR290 pKa = 11.84 RR291 pKa = 11.84 AARR294 pKa = 11.84 SAGPGAQGRR303 pKa = 11.84 GRR305 pKa = 11.84 RR306 pKa = 11.84 CRR308 pKa = 11.84 PDD310 pKa = 3.02 QAASTLAPILIVRR323 pKa = 11.84 GNGNGLKK330 pKa = 9.88 CLRR333 pKa = 11.84 YY334 pKa = 8.9 RR335 pKa = 11.84 WHH337 pKa = 7.54 RR338 pKa = 11.84 DD339 pKa = 2.7 HH340 pKa = 7.09 SALFTSVSTTFRR352 pKa = 11.84 WLRR355 pKa = 11.84 AGLTDD360 pKa = 3.47 RR361 pKa = 11.84 EE362 pKa = 4.47 GNAEE366 pKa = 4.07 VIVRR370 pKa = 11.84 FNTDD374 pKa = 2.45 KK375 pKa = 10.91 QRR377 pKa = 11.84 EE378 pKa = 4.12 DD379 pKa = 3.56 VLDD382 pKa = 4.42 CGAVPEE388 pKa = 4.51 TVQYY392 pKa = 11.36 LCGTMPFFSS401 pKa = 4.06
Molecular weight: 45.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.005
IPC2_protein 8.141
IPC_protein 8.083
Toseland 7.995
ProMoST 8.521
Dawson 8.756
Bjellqvist 8.946
Wikipedia 8.77
Rodwell 8.785
Grimsley 7.951
Solomon 8.858
Lehninger 8.843
Nozaki 9.004
DTASelect 8.741
Thurlkill 8.77
EMBOSS 8.916
Sillero 9.033
Patrickios 4.495
IPC_peptide 8.858
IPC2_peptide 7.892
IPC2.peptide.svr19 7.953
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2415
87
660
402.5
44.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.578 ± 0.431
2.195 ± 0.62
6.418 ± 0.472
6.625 ± 0.619
3.106 ± 0.278
7.246 ± 0.561
2.153 ± 0.241
4.265 ± 0.634
4.224 ± 0.596
7.495 ± 0.855
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.029 ± 0.416
3.271 ± 0.463
6.791 ± 1.204
3.644 ± 0.449
7.081 ± 0.876
7.039 ± 0.299
6.791 ± 0.641
7.288 ± 0.887
1.491 ± 0.24
3.271 ± 0.334
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here