Teladorsagia circumcincta (Brown stomach worm) (Ostertagia circumcincta)
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 25373 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2G9UR19|A0A2G9UR19_TELCI SEP domain protein OS=Teladorsagia circumcincta OX=45464 GN=TELCIR_05363 PE=3 SV=1
MM1 pKa = 7.91 DD2 pKa = 5.37 PVGSDD7 pKa = 3.27 ATGLTPMDD15 pKa = 4.36 PVGSDD20 pKa = 3.26 ATGLTPMDD28 pKa = 4.24 PVASGDD34 pKa = 3.82 TVLTPMLPTTSDD46 pKa = 3.0 ATGLTFTDD54 pKa = 3.96 PTGSDD59 pKa = 3.04 ATGLTFTDD67 pKa = 4.23 PAGSDD72 pKa = 3.22 ATGLTFTDD80 pKa = 4.43 PADD83 pKa = 3.59 SDD85 pKa = 4.35 AAGLTPMDD93 pKa = 4.09 PTGSDD98 pKa = 3.26 NTGLPPVDD106 pKa = 3.54 PTGSDD111 pKa = 3.39 VTGLIPIDD119 pKa = 3.64 PTGSDD124 pKa = 3.62 VTGLTPMDD132 pKa = 3.75 PTGSDD137 pKa = 3.52 VTGLTPMDD145 pKa = 3.97 PTGSDD150 pKa = 3.27 ATGLTPMDD158 pKa = 4.63 SGCSDD163 pKa = 3.22 AAGFGVYY170 pKa = 9.43 EE171 pKa = 3.7 NHH173 pKa = 5.71 FRR175 pKa = 11.84 EE176 pKa = 4.69 VEE178 pKa = 4.22 HH179 pKa = 6.69 CAHH182 pKa = 7.14 GYY184 pKa = 10.65 GDD186 pKa = 4.06 DD187 pKa = 3.65 RR188 pKa = 11.84 HH189 pKa = 7.27 NEE191 pKa = 4.14 DD192 pKa = 3.75 VLHH195 pKa = 6.49 DD196 pKa = 3.94 HH197 pKa = 7.12 SRR199 pKa = 11.84 DD200 pKa = 3.16 CDD202 pKa = 3.79 YY203 pKa = 11.34 VHH205 pKa = 7.33 GYY207 pKa = 9.38 LRR209 pKa = 11.84 YY210 pKa = 9.6 VPLLVKK216 pKa = 9.15 EE217 pKa = 4.39 TTPVRR222 pKa = 11.84 EE223 pKa = 4.23 DD224 pKa = 3.55 SVPQHH229 pKa = 6.37 IDD231 pKa = 3.23 EE232 pKa = 5.3 PGHH235 pKa = 5.22 YY236 pKa = 10.19 SRR238 pKa = 11.84 VPDD241 pKa = 4.2 ANQLEE246 pKa = 4.46
Molecular weight: 25.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.667
IPC2_protein 3.681
IPC_protein 3.732
Toseland 3.49
ProMoST 3.897
Dawson 3.745
Bjellqvist 3.923
Wikipedia 3.719
Rodwell 3.554
Grimsley 3.389
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.177
Thurlkill 3.554
EMBOSS 3.732
Sillero 3.859
Patrickios 1.214
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.768
Protein with the highest isoelectric point:
>tr|A0A2G9UV75|A0A2G9UV75_TELCI PQ loop repeat protein OS=Teladorsagia circumcincta OX=45464 GN=TELCIR_03938 PE=4 SV=1
MM1 pKa = 7.32 KK2 pKa = 10.15 RR3 pKa = 11.84 GAAGKK8 pKa = 9.74 SSKK11 pKa = 10.26 SSKK14 pKa = 9.36 SSKK17 pKa = 9.25 SRR19 pKa = 11.84 RR20 pKa = 11.84 AGSGKK25 pKa = 9.78 SGKK28 pKa = 8.75 SSRR31 pKa = 11.84 SRR33 pKa = 11.84 GSKK36 pKa = 9.42 SGRR39 pKa = 11.84 SRR41 pKa = 11.84 RR42 pKa = 11.84 KK43 pKa = 8.77 GKK45 pKa = 9.65 SSRR48 pKa = 11.84 SSRR51 pKa = 11.84 SKK53 pKa = 10.01 SRR55 pKa = 11.84 RR56 pKa = 11.84 SKK58 pKa = 10.67 GSRR61 pKa = 11.84 SSKK64 pKa = 9.07 SRR66 pKa = 11.84 RR67 pKa = 11.84 GRR69 pKa = 11.84 SKK71 pKa = 10.49 SSKK74 pKa = 9.3 SKK76 pKa = 10.1 SGRR79 pKa = 11.84 SRR81 pKa = 11.84 KK82 pKa = 8.35 SRR84 pKa = 11.84 RR85 pKa = 11.84 SRR87 pKa = 11.84 KK88 pKa = 6.72 SQRR91 pKa = 11.84 GSRR94 pKa = 11.84 SRR96 pKa = 11.84 RR97 pKa = 11.84 GKK99 pKa = 9.29 SRR101 pKa = 11.84 RR102 pKa = 11.84 SKK104 pKa = 10.52 SSRR107 pKa = 11.84 SSKK110 pKa = 10.09 SKK112 pKa = 10.2 SRR114 pKa = 11.84 RR115 pKa = 11.84 SKK117 pKa = 10.45 SKK119 pKa = 10.26 RR120 pKa = 11.84 EE121 pKa = 3.84 SGSRR125 pKa = 11.84 RR126 pKa = 11.84 SKK128 pKa = 9.94 SSRR131 pKa = 11.84 SARR134 pKa = 11.84 TTPGSCC140 pKa = 4.06
Molecular weight: 15.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.286
IPC_protein 12.866
Toseland 13.042
ProMoST 13.525
Dawson 13.042
Bjellqvist 13.027
Wikipedia 13.51
Rodwell 12.778
Grimsley 13.086
Solomon 13.525
Lehninger 13.437
Nozaki 13.042
DTASelect 13.027
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.486
IPC_peptide 13.539
IPC2_peptide 12.515
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
25373
0
25373
5444164
20
4415
214.6
24.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.425 ± 0.017
2.056 ± 0.012
5.617 ± 0.015
6.798 ± 0.019
3.937 ± 0.012
6.149 ± 0.025
2.393 ± 0.008
5.227 ± 0.012
5.888 ± 0.018
8.622 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.727 ± 0.01
4.021 ± 0.012
5.284 ± 0.024
3.868 ± 0.014
6.13 ± 0.019
7.321 ± 0.018
5.591 ± 0.02
6.7 ± 0.015
1.162 ± 0.007
3.084 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here