Fluviicoccus keumensis
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3271 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V2G3V4|A0A4V2G3V4_9GAMM Basal-body rod modification protein FlgD OS=Fluviicoccus keumensis OX=1435465 GN=EV700_2430 PE=3 SV=1
MM1 pKa = 7.29 SHH3 pKa = 6.97 LPSAQEE9 pKa = 4.61 LIALDD14 pKa = 4.22 EE15 pKa = 4.37 FLQSDD20 pKa = 4.71 RR21 pKa = 11.84 CPPDD25 pKa = 3.42 TFNLAALRR33 pKa = 11.84 GFIWAVLASPVPLAPDD49 pKa = 2.77 EE50 pKa = 4.08 WMPLIWEE57 pKa = 4.45 GEE59 pKa = 4.07 EE60 pKa = 4.78 GEE62 pKa = 4.36 EE63 pKa = 4.34 NPRR66 pKa = 11.84 FHH68 pKa = 7.99 NEE70 pKa = 3.42 EE71 pKa = 3.72 EE72 pKa = 4.2 AGMVMGTIIALYY84 pKa = 10.15 DD85 pKa = 3.27 HH86 pKa = 7.46 AAQLVEE92 pKa = 5.6 DD93 pKa = 4.16 NASPIPADD101 pKa = 3.45 YY102 pKa = 11.14 AFNADD107 pKa = 3.79 PEE109 pKa = 4.57 LMVDD113 pKa = 5.7 LIDD116 pKa = 3.55 WCQGFLAGHH125 pKa = 7.16 DD126 pKa = 3.54 WLQQVWDD133 pKa = 4.3 QVCEE137 pKa = 4.2 AYY139 pKa = 10.52 AKK141 pKa = 10.73 VADD144 pKa = 4.01 AEE146 pKa = 4.6 INLSDD151 pKa = 5.02 EE152 pKa = 4.23 INNVINVVGLFAGYY166 pKa = 9.78 PEE168 pKa = 4.5 VLDD171 pKa = 4.53 HH172 pKa = 7.4 LEE174 pKa = 4.57 DD175 pKa = 4.23 PQAVAADD182 pKa = 4.02 LPMVAKK188 pKa = 10.51 DD189 pKa = 3.88 VLPQALLAYY198 pKa = 9.94 ARR200 pKa = 11.84 TGLEE204 pKa = 3.87 FCDD207 pKa = 3.68 EE208 pKa = 4.04 QMEE211 pKa = 4.27 MEE213 pKa = 4.16 AAEE216 pKa = 4.21 NEE218 pKa = 4.53 GG219 pKa = 3.49
Molecular weight: 24.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.694
IPC_protein 3.668
Toseland 3.478
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.554
Rodwell 3.503
Grimsley 3.389
Solomon 3.643
Lehninger 3.592
Nozaki 3.77
DTASelect 3.948
Thurlkill 3.516
EMBOSS 3.567
Sillero 3.795
Patrickios 0.769
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.72
Protein with the highest isoelectric point:
>tr|A0A4Q7ZAD6|A0A4Q7ZAD6_9GAMM Iron-binding protein IscA OS=Fluviicoccus keumensis OX=1435465 GN=EV700_0515 PE=3 SV=1
MM1 pKa = 7.67 PKK3 pKa = 10.09 KK4 pKa = 10.53 SPQRR8 pKa = 11.84 KK9 pKa = 7.88 HH10 pKa = 5.99 RR11 pKa = 11.84 LRR13 pKa = 11.84 RR14 pKa = 11.84 QLLQKK19 pKa = 10.25 HH20 pKa = 5.34 QRR22 pKa = 11.84 QQQQLRR28 pKa = 11.84 RR29 pKa = 11.84 QQWFLLLQPQPLLPLAALQPGQLQPWPALTT59 pKa = 3.82
Molecular weight: 7.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.038
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.501
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.237
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.083
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3271
0
3271
1113237
31
6807
340.3
37.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.946 ± 0.048
0.977 ± 0.016
5.489 ± 0.03
5.668 ± 0.043
3.662 ± 0.027
7.877 ± 0.048
2.289 ± 0.026
4.828 ± 0.033
3.699 ± 0.037
11.125 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.535 ± 0.024
3.164 ± 0.035
5.082 ± 0.035
4.007 ± 0.028
6.599 ± 0.047
5.537 ± 0.043
5.251 ± 0.063
7.248 ± 0.04
1.435 ± 0.016
2.582 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here